A semi-automated method for the determination of glycosylated haemoglobin

Ross, I.S.; Gibson, P.F.

doi:10.1016/0009-8981(79)90165-7

Cited by 26 publications

(5 citation statements)

References 12 publications

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“…All patients had a peripheral blood profile performed (Coulter Counter model S calibrated with Coulter 4C) on a venous sample (K2EDTA, 1 mg/ml as anticoagulant) within 4 h of venepuncture and a random plasma glucose determined by a glucose-oxidase method. In a further 15 diabetic patients (seven males; eight females), in whom the MCV ranged from 76 to 94 r, haemoglobin A1 c (HbAlc) was also measured by a semi-automated technique based on the furfuraldehyde/thiobarbituric acid reaction [2].…”

Section: Methodsmentioning

confidence: 99%

The mean red cell volume in diabetes mellitus

1981

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Section: Methodsmentioning

confidence: 99%

The mean red cell volume in diabetes mellitus

1981

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“…Samples were taken in hospital in the mid-morning, after the diabetic patients had their normal insulin and breakfast at home. All were rested for at least 30 rain in a room whose ambient temperature was kept between 20.5 ~ and 24.4 ~ At the start of the test, a blood sample was taken with minimal venous compression for basal fibrinolysis and fibronectin, and for glycosylated haemoglobin level, assayed by the thiobarbituric method [20] and plasma glucose level measured by a glucose oxidase method (GOD-PERID, Boehringer, Mannheim, FRG).…”

Section: Blood Samplingmentioning

confidence: 99%

Abnormal endothelial release of fibrinolytic activity and fibronectin in diabetic microangiopathy

et al. 1984

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Endothelial cell function in Type 1 (insulin-dependent) diabetic patients, both with and without retinopathy, was assessed by measuring the plasma fibrinolytic activity and fibronectin after 10 min venous stasis induced by a sphygmomanometer cuff. After venous stasis, diabetic subjects with proliferative retinopathy had fibrinolytic responses (median 0.13 increasing to 0.26 U/ml) in the low normal range, which were significantly less (p less than 0.005) than control subjects (0.17-0.68 U/ml) and diabetic patients with minimal retinopathy (0.16-0.68 U/ml; p less than 0.01). Plasma fibronectin levels were similar in the different groups, but after venous stasis, rose significantly in the diabetic patients, both in those with proliferative retinopathy (mean 317-399 micrograms/ml; p less than 0.002) and without retinopathy (312-371 micrograms/ml; p less than 0.05) but not in normal subjects (304-333 micrograms/ml). These changes in fibrinolytic activity and fibronectin were independent of blood glucose, glycosylated haemoglobin, or indices of sensory or autonomic nerve function. These disturbances of endothelial function, together with known abnormalities of haemostatic variables and microvascular reflexes, might convert a usually temporary obstruction of capillary blood flow into a pathological capillary closure, and might contribute to the inexorable progression of advanced diabetic microangiopathy in spite of good diabetic control.

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“…These methods were used at room temperature and include the Schiff bases (i. e. temporary adducts) in the estimations. Colorimetric methods, based on that of Fliickiger and Winterhalter [5], were used by Laboratories 2, 6 and 7 [6,7]. The colour developed by reaction of the hydroxymethylfurfural (5-HMF) (produced by boiling haemoglobin in dilute oxalic acid) with thio-barbituric acid is mainly due to the presence of HbA1~.…”

mentioning

confidence: 99%

“…The colour developed by reaction of the hydroxymethylfurfural (5-HMF) (produced by boiling haemoglobin in dilute oxalic acid) with thio-barbituric acid is mainly due to the presence of HbA1~. Laboratories 2, 6 and 7 each compared the values for their fructose or 5-HMF standards with column separation values (either of bloods used as standards, or of an 'in-house' series of bloods) in order to express the measured 5-HMF as percentage total glycosylated haemoglobin (ketoamine form), having previously demonstrated significant correlation between the column separation HbA1 results and 5-HMF levels in an 'in-house' series of blood samples [7]. Laboratory 3 used electroendosmosis [8] on gel plates, in which the HbA2 moves through the charged hydroxyl groups present in the gel during electrophoresis.…”

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confidence: 99%

A collaborative study of the measurement of glycosylated haemoglobin by several methods in seven laboratories in the United Kingdom

Boucher

Burrin²,

Gould³

et al. 1983

Diabetologia

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A collaborative study compared methods for measuring glycosylated haemoglobin in seven laboratories in the United Kingdom. No satisfactory standard for general use was found. Satisfactory internal quality control systems were in use for each assay which allowed the maintenance of a normal range in each participating laboratory. No satisfactory quality control system suitable for general use could be identified. Costs and convenience of the assays are reported. The technical problems with each type of assay and precautions for their use were identified, such as the need for standardization in incubation times, the practicability of automation of colorimetric assays, and the precision of pH needed for buffers in column separation methods. The relevance of the technical problems to interpretation of measurements is also considered. It is concluded that laboratories measuring glycosylated haemoglobin should maintain a normal range, use 'in-house' quality controls to monitor assay performance and keep clinical colleagues informed of the findings and of any changes in methodology that might affect the interpretation of results.

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A semi-automated method for the determination of glycosylated haemoglobin

Cited by 26 publications

References 12 publications

The mean red cell volume in diabetes mellitus

The mean red cell volume in diabetes mellitus

Abnormal endothelial release of fibrinolytic activity and fibronectin in diabetic microangiopathy

A collaborative study of the measurement of glycosylated haemoglobin by several methods in seven laboratories in the United Kingdom

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