A SEM-correlation of the anatomy of the vitreous body: Making visible the invisible

Jongebloed, W.L.; Humalda, D.; Worst, J.F.G.

doi:10.1007/bf00166693

Cited by 9 publications

(3 citation statements)

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“…The specimens obtained in this way were processed for SEM in the usual way (Jongebloed et al, 1986). While keeping the preparation constantly submerged, ink of different colours (and of different particle size) is injected from various points into the vitreous body in order to visualize the individual internal spaces (cisterns).…”

Section: Methodsmentioning

confidence: 99%

The cisternal anatomy of the vitreous body

Jongebloed

Worst²

1987

Doc Ophthalmol

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The vitreous body of a fresh human eye removed post-mortem was suspended in water after stripping off the corneo-scleral coat, the choroid and the retina. Coloured ink was injected into the vitreous specimen from different points. Individual spaces (cisterns), transitions between cisterns and specialized structures like the bursa premacularis and the canal of Cloquet became visible when partial filling had taken place. SEM-examination of the individual cisternal walls of the bursa premacularis area showed networks of different density and texture and a considerable variation in the length of the individual fibres, some of which extended from the ciliary body (Retzius bundles) to the rim of the Bursa premacularis.

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Section: Methodsmentioning

confidence: 99%

The cisternal anatomy of the vitreous body

Jongebloed

Worst²

1987

Doc Ophthalmol

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“…Lamellae originate from the anterior hyaloid lamina and vitreous base, insert themselves around the optic disc, and consist of a locally increased packing of the matrix. The presence of lamellae was confirmed in a SEM-study on the rabbit vitreous (Jongebloed, Humalda and Worst 1986).…”

Section: Introductionmentioning

confidence: 92%

Organization of the Rabbit Vitreous Body: Lamellae, Cloquet's Channel and a Novel Structure, the ‘Alae Canalis Cloqueti’

Los¹,

Luyn

Nieuwenhuis

1999

Experimental Eye Research

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“…Sebag and Balazs reported observing a large bundle of prominent fibers coursing anteroposteriorly and entering the retrohyaloid space via the premacular hole in a vitreous specimen [20]. Using electron microscopy, Jongebloed et al reportedly observed the BPM displayed as a collagenous structure that was denser than other regions [21]. The fact that fluorescein staining of the posterior wall of the PPVP, which we firmly believe is the BMP adhered on the PVC, appears to also support our assumption that the BPM is a gel-like parenchymal tissue.…”

Section: Discussionmentioning

confidence: 99%

Involvement of premacular mast cells in the pathogenesis of macular diseases

et al. 2019

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We previously reported on the elevated intravitreal activities of tryptase and chymase in association with idiopathic epiretinal membrane (ERM) and idiopathic macular hole (MH). In this present study, we investigated the potential intraocular production of these serine proteases, and measured and compared tryptase and chymase activities in the vitreous body and serum in ERM, MH, proliferative diabetic retinopathy (PDR), and rhegmatogenous retinal detachment (RRD) patients. In addition, nuclear staining with hematoxylin and eosin (H&E) and mast-cell staining with toluidine blue were performed on samples of the vitreous core and bursa premacularis (BPM) of MH. We also performed immunostaining on the above two regions of vitreous samples for MH with anti-tryptase antibody, anti-chymase antibody, anti-podoplanin antibody, anti-lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) antibody, and anti-fibroblast antibody. Moreover, we performed immunostaining with anti-tryptase antibody and anti-chymase antibody on ERMs collected intraoperatively. Tryptase activity in the vitreous body was significantly higher in ERM and MH than in PDR. However, no significant differences were observed in the tryptase activity in the serum among these four diseases. Chymase activity in the vitreous body was significantly higher in MH than in the other three diseases, yet chymase activity in the serum was below detection limit in any of the diseases. Nuclear staining with H&E revealed an abundance of nuclei in the BPM region, but few in the surrounding area. Mast-cell staining with toluidine blue revealed that the BPM showed metachromatic staining. In immunostaining with anti-fibroblasts antibody, anti-tryptase antibody, anti-chymase antibody, anti-podoplanin antibody, and anti-LYVE-1 antibody, the BPM stained more strongly than the vitreous core. Tryptase and chymase-positive cells were also observed in ERM. These findings revealed that the presence of mast cells in the BPM potentially represent the source of these serine proteases. Moreover, the BPM, as a lymphatic tissue, may play an important role in the pathogenesis of macular disease.

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A SEM-correlation of the anatomy of the vitreous body: Making visible the invisible

Cited by 9 publications

References 0 publications

The cisternal anatomy of the vitreous body

The cisternal anatomy of the vitreous body

Organization of the Rabbit Vitreous Body: Lamellae, Cloquet's Channel and a Novel Structure, the ‘Alae Canalis Cloqueti’

Involvement of premacular mast cells in the pathogenesis of macular diseases

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