Organization of the Rabbit Vitreous Body: Lamellae, Cloquet's Channel and a Novel Structure, the ‘Alae Canalis Cloqueti’

Los, Leonoor I.; Luyn, Marja J. A. van; Nieuwenhuis, Paul

doi:10.1006/exer.1999.0708

Cited by 29 publications

(24 citation statements)

References 9 publications

(7 reference statements)

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“…In observation by LM, it is surrounded by a regular matrix. 25 These aspects are most consistent with a tissue specialization. Our data indicate that Cloquet's channel develops between 2 weeks and 5 months of age in rabbits.…”

Section: Discussionsupporting

confidence: 66%

“…Lamellae seem to consist of parallel-running fibres when viewed by LM, but appear to consist of fibre networks after TEM. 25 Therefore, lamellae and the surrounding matrix have an essentially similar network structure, the main differences being mesh diameter and predominant fibre orientation.…”

Section: Discussionmentioning

confidence: 99%

“…This structure is generally known as Cloquet's channel. 27 Wing-like lamellar structures, the 'alae canalis Cloqueti', 25 extend from this channel and insert themselves at the medullary rays. In humans, Cloquet's channel and its alae were not seen.…”

Section: Specific Structuresmentioning

confidence: 99%

“…Dyes (Magic Colour, Royal Sovereign, London, UK) were injected to show areas with a very loosemeshed matrix structure or consisting of fluid-filled spaces. 25,26 Dyes are suspensions of particles with diameters of approximately 0.1-0.5 mm. 26 For both preparation methods, specimens were kept submerged in a buffer solution and were studied using a stereo microscope (Wild, type M-650).…”

Section: Anatomymentioning

confidence: 99%

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The rabbit as an animal model for post-natal vitreous matrix differentiation and degeneration

Los

2008

Eye

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Purpose This study evaluates whether rabbits are a suitable animal model to study post-natal vitreous differentiation and degeneration. Methods Human and rabbit eyes of various ages were studied by complementary anatomical techniques, light microscopy, and transmission electron microscopy. Results The global vitreous matrix organization is similar in human and rabbit eyes, lamellae are an important aspect thereof and show striking morphological changes with increasing age. In humans, liquefaction is more conspicuous than in rabbits but changes in matrix histology consistent with liquefaction can also be observed in the latter. Conclusions Lamellar development is consistent with vitreous differentiation, while increasing liquefaction is consistent with matrix degeneration. At the anatomical and histological levels, human and rabbit vitreous matrices are sufficiently similar to make the rabbit a promising animal model for the study of the pathogenesis of vitreous matrix differentiation and degeneration in more detail.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Specific Structuresmentioning

confidence: 99%

Section: Anatomymentioning

confidence: 99%

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The rabbit as an animal model for post-natal vitreous matrix differentiation and degeneration

Los

2008

Eye

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“…On the other hand, the outflow route of smaller-diameter nanospheres may be the retina as well as the anterior chamber angle. Los et al [14] evaluated the structure of the rabbit vitreous histologically in detail and indicated that a lamellar structure, named the hyaloid lamina, could interfere with movements of substances introduced into the vitreous cavity. From the histological results in this study, we could demonstrate that nanoparticles could go into the retina through the structure.…”

Section: Discussionmentioning

confidence: 99%

Effect of Particle Size of Polymeric Nanospheres on Intravitreal Kinetics

Sakurai¹,

Ozeki²,

Kunou³

et al. 2000

Ophthalmic Res

136

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In this study, we injected nanospheres containing a fluorescein derivative into the vitreous cavity of pigmented rabbit eyes and evaluated their intraocular kinetics as drug carriers in vivo. Polystyrene nanospheres (2 µm, 200 nm and 50 nm in diameter) containing a fluorescein derivative were used in this study. A suspension of each particle was prepared by diluting with distilled water at a concentration of 10 µg/ml equivalent to sodium fluorescein. The suspension of nanospheres was injected once into the vitreous cavity of unilateral eyes of pigmented rabbits. A sodium fluorescein solution of the same concentration was injected once into the vitreous cavity of the other eye as the control. The intraocular kinetics of nanospheres was evaluated by measuring vitreous fluorescence using a scanning fluorophotometer. To investigate elimination pathways of nanospheres in detail, serial cross-sections of the eyes were examined with a fluorescence microscope. The fluorescence derived from nanospheres was observed in the vitreous cavity for over 1 month (2 µm: t_1/2 = 5.4 ± 0.8 days, 200 nm: t_1/2 = 8.6 ± 0.7 days, 50 nm: t_1/2 = 10.1 ± 1.8 days), whereas that in the control eyes completely disappeared within 3 days (t_1/2 = 7.8 ± 0.7 h). The elimination half-life from the vitreous cavity correlated well with the particle diameter (r = –0.997, p = 0.007). Histological studies using a fluorescence microscope revealed that nanospheres with a diameter of 2 µm were seen in the vitreous cavity and trabecular meshwork, while nanospheres with a diameter of smaller than 200 nm were also observed in the retina as well as these tissues. Our findings indicated that nanospheres may be beneficial as a drug carrier to the retina, vitreous and trabecular meshwork.

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Ocular System

2012

Comparative Pathophysiology and Toxicology of Cyclooxygenases

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Organization of the Rabbit Vitreous Body: Lamellae, Cloquet's Channel and a Novel Structure, the ‘Alae Canalis Cloqueti’

Cited by 29 publications

References 9 publications

The rabbit as an animal model for post-natal vitreous matrix differentiation and degeneration

The rabbit as an animal model for post-natal vitreous matrix differentiation and degeneration

Effect of Particle Size of Polymeric Nanospheres on Intravitreal Kinetics

Ocular System

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