A Selective Liquid Chromatography Assay for the Determination of d1-α-Tocopherol Acetate in Plasma Samples

Development of a validated liquid chromatography method for the simultaneous determination of eight fat-soluble vitamins in biological fluids after solid-phase extraction

“…Deproteinization of the plasma is mainly achieved by ACN, EtOH, MeOH, and liquid-liquid extraction for sample clean-up is performed by n-hexane or heptane [9,17,[21][22][23][24][25][26][27][28][29]. Sometimes, sample preparation requires perchloric acid or trichloroacetic acid deproteinization of the plasma followed by centrifugation and filtration through a disposable filter [12].…”