“…Deproteinization of the plasma is mainly achieved by ACN, EtOH, MeOH, and liquid-liquid extraction for sample clean-up is performed by n-hexane or heptane [9,17,[21][22][23][24][25][26][27][28][29]. Sometimes, sample preparation requires perchloric acid or trichloroacetic acid deproteinization of the plasma followed by centrifugation and filtration through a disposable filter [12].…”