A second proteinase encoded by a plant potyvirus genome.

Carrington, James C.; Cary, Susan M.; Parks, T. Dawn; Dougherty, William G.

doi:10.1002/j.1460-2075.1989.tb03386.x

Cited by 272 publications

(179 citation statements)

References 26 publications

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“…1), similar to those characterized for tobacco etch virus (TEV [9,10,14,31]), that would result in production of mature proteins (P1, HC-Pro, P3, 6K1, CI, 6K2, VPg-NIa, NIb, and CP) that also are encoded by other species of the genera Rymovirus, Potyvirus, and "Ipomovirus". Two motifs that are conserved among all potyviruses, the polymerase motif of the NIb protein and the nucleotide binding motif of the CI protein, occur in the WSMV polyprotein sequence at the expected map positions (Fig.…”

Section: Resultsmentioning

confidence: 80%

Phylogenetic Relationships Within the Family Potyviridae: Wheat Streak Mosaic Virus and Brome Streak Mosaic Virus Are Not Members of the Genus Rymovirus

Stenger¹,

Hall²,

Choi³

et al. 1998

Phytopathology®

123

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The complete nucleotide sequence of wheat streak mosaic virus (WSMV) has been determined based on complementary DNA clones derived from the 9,384-nucleotide (nt) RNA of the virus. The genome of WSMV has a 130-nt 5′ leader and 149-nt 3′-untranslated region and is polyadenylated at the 3′ end. WSMV RNA encodes a single polyprotein of 3,035 amino acid residues and has a deduced genome organization typical for a member of the family Potyviridae (5′-P1/HC-Pro/P3/6K1/CI/6K2/VPg-NIa/NIb/CP-3′). Because WSMV shares with ryegrass mosaic virus (RGMV) the biological property of transmission by eriophyid mites, WSMV has been assigned to the genus Rymovirus, of which RGMV is the type species. Phylogenetic analyses were conducted with complete polyprotein or NIb protein sequences of 11 members of the family Potyviridae, including viruses of monocots or dicots and viruses transmitted by aphids, whiteflies, and mites. WSMV and the monocot-infecting, mite-transmitted brome streak mosaic virus (BrSMV) are sister taxa and share a most recent common ancestor with the whitefly-transmitted sweet potato mild mottle virus, the type species of the proposed genus “Ipomovirus.” In contrast, RGMV shares a most recent common ancestor with aphid-transmitted species of the genus Potyvirus. These results indicate that WSMV and BrSMV should be classified within a new genus of the family Potyviridae and should not be considered species of the genus Rymovirus.

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Section: Resultsmentioning

confidence: 80%

Phylogenetic Relationships Within the Family Potyviridae: Wheat Streak Mosaic Virus and Brome Streak Mosaic Virus Are Not Members of the Genus Rymovirus

Stenger¹,

Hall²,

Choi³

et al. 1998

Phytopathology®

123

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“…This initial translation product is proteolytically cleaved into eight mature proteins (Dougherty and Carrington, 1988). At least two of the mature TEV products (Nla and HC-Pro) function as proteinases that catalyze most of the polyprotein processing (Carrington and Dougherty, 1987;Carrington et al, 1989). At least three potyviral proteins (CI, Nla, Nlb) replicase complex (Domier et al, 1987;Dougherty and Carrington, 1988).…”

Section: Introductionmentioning

confidence: 99%

Nuclear Transport of Plant Potyviral Proteins

Restrepo¹,

Freed²,

Carrington³

1990

The Plant Cell

138

180

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We have used immunoblotting, immunocytochemical, and gene fusion methods to examine the differential subcellular partitioning of tobacco etch potyvirus proteins that are potentially associated with RNA replication. From the earliest timepoints at which viral proteins could be detected, proteins Nla (49-kilodalton proteinase) and Nlb (58-kilodalton polymerase) were localized primarily in the nucleus, whereas the 71-kilodalton cylindrical inclusion protein was identified in the cytoplasm. The Nla and Nlb coding regions were fused to the 8-glucuronidase (GUS) sequence in a plant expression vector, resulting in synthesis of chimeric proteins in transfected protoplasts and in transgenic plants. In situ localization of GUS activity revealed nuclear localization of the GUS-Nla and GUS-NIb fusion proteins and cytoplasmic localization of nonfused GUS. These results indicate that both Nla and Nlb contain nuclear targeting signals, and that they may serve as useful models for studies of plant cell nuclear transport. A discussion of the general utility of the nuclear transport system described here, as well as the role of nuclear translocation of potyviral proteins, is presented.

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“…Secondly, contrary to the assumption we made during our search, the cleavage sites of GCMV might not be colinear with those of other picorna-like viruses. Thirdly, another viral protein could act as a protease with a different specificity, as reported in picomaviruses and recently also in potyviruses (51).…”

Section: Nucleic Acids Researchmentioning

confidence: 88%

Nucleotide sequence of Hungarian grapevine chrome mosaic nepovirus RNA1

Gall¹,

Candresse²,

Brault³

et al. 1989

Nucl Acids Res

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The nucleotide sequence of the RNAl of hungarian grapevine chrome mosaic virus, a nepovirus very closely related to tomato black ring virus, has been determined from cDNA clones. It is 7212 nucleotides in length excluding the 3' terminal poly(A) tail and contains a large open reading frame extending from nucleotides 216 to 6971. The presumably encoded polyprotein is 2252 amino acids in length with a molecular weight of 250 kDa. The primary structure of the polyprotein was compared with that of other viral polyproteins, revealing the same general genetic organization as that of other picorna-like viruses (comoviruses, potyviruses and picornaviruses), except that an additional protein is suspected to occupy the N-terminus of the polyprotein.

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A second proteinase encoded by a plant potyvirus genome.

Cited by 272 publications

References 26 publications

Phylogenetic Relationships Within the Family Potyviridae: Wheat Streak Mosaic Virus and Brome Streak Mosaic Virus Are Not Members of the Genus Rymovirus

Phylogenetic Relationships Within the Family Potyviridae: Wheat Streak Mosaic Virus and Brome Streak Mosaic Virus Are Not Members of the Genus Rymovirus

Nuclear Transport of Plant Potyviral Proteins

Nucleotide sequence of Hungarian grapevine chrome mosaic nepovirus RNA1

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