Nucleotide sequence of Hungarian grapevine chrome mosaic nepovirus RNA1

Abstract

The nucleotide sequence of the RNAl of hungarian grapevine chrome mosaic virus, a nepovirus very closely related to tomato black ring virus, has been determined from cDNA clones. It is 7212 nucleotides in length excluding the 3' terminal poly(A) tail and contains a large open reading frame extending from nucleotides 216 to 6971. The presumably encoded polyprotein is 2252 amino acids in length with a molecular weight of 250 kDa. The primary structure of the polyprotein was compared with that of other viral polyproteins, revealing the same general genetic organization as that of other picorna-like viruses (comoviruses, potyviruses and picornaviruses), except that an additional protein is suspected to occupy the N-terminus of the polyprotein.

Nucleotide sequence of tomato ringspot virus RNA1

“…Fig. 2 aligns the motifs identified in the TomRSV polyprotein with those present in the polyproteins encoded by RNA1 of the nepoviruses tomato black ring virus (TBRV; Greif et al, 1988), grapevine chrome mosaic virus (GCMV; Le Gall et al, 1989), and grapevine fanleaf virus (GFLV; Ritzenthaler et al, 1991), as well as B RNA of the comovirus cowpea mosaic virus (CPMV; Lomonossoff & Shanks, 1983), 0001-2582 © 1995 Crown Copyright, Government of Canada…”

“…Consequently, assignment of cleavage sites for delineating protein-coding regions was based on the known cleavage sites (Q/S, Q/M, Q/G, E/G and E/S) commonly used for maturation of como-, poty-and picornavirus polyproteins (Hellen et al, 1989;Palmenberg, 1990;Wellink et al, 1986). The locations of these sites were then compared with the locations of known cleavage sites for CPMV, as well as the putative sites for the RNAl-encoded polyproteins of the nepoviruses TBRV, GCMV and GFLV Ritzenthaler et al, 1991 ;Le Gall et al, 1989;Greif et al, 1988;Margis et al, 1994). Fig.…”

Nucleotide sequence of tomato ringspot virus RNA-2

“…The 5' non-coding region of TomRSV RNA-2 was analysed and compared to the corresponding regions of the two RNA components of TBRV (Meyer et al, 1986;Greif et al, 1988) and GCMV Le Gall et al, 1989), RNA-2 of GFLV (Serghini et al, 1990) and the B and M components of the comovirus CPMV (Lomonossoff & Shanks, 1983;van Wezenbeek et al, 1983). It has been reported previously that TBRV, GCMV, GFLV and CPMV share a conserved UGAAAAAU sequence downstream from the 5' terminus (Serghini et al, 1990).…”

Immunodetection of the proteins encoded by grapevine chrome mosaic nepovirus RNA2

“…The genome of Hungarian grapevine chrome mosaic nepovirus (GCMV) (Martelli & Quacquarelli, 1972) consists of two positive-sense ssRNAs of 7212 nucleotides (RNA1 ; Le Gall et al, 1989) and 4441 nucleotides (RNA2; Brault et al, 1989). These RNAs are polyadenylated at their 3' ends and, similarly to those of the closely related tomato black ring nepovirus (TBRV) (Mayo et al, 1982), are probably covalently linked to a small protein (VPg) at their 5' end.…”

“…Indeed, the polyproteins encoded by RNA 1 of different nepoviruses have the characteristic signature sequences of proteins involved in viral RNA replication (Greif et al, 1988;Le Gall et al, 1989;Ritzenthaler et al, 1991). RNA 1 also encodes a proteolytic activity responsible for the maturation of both RNAI-and RNA2-encoded polyproteins (Demangeat et al, , 1991.…”