A satellite cell mitogen from crushed adult muscle

Bischoff, Richard

doi:10.1016/0012-1606(86)90235-6

Cited by 251 publications

(173 citation statements)

References 31 publications

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“…The collagen-extract matrix was added to the minced muscle by placing 3 strips of matrix about 2 mm x 10 mm each on top of the mince in the muscle bed before suturing the skin. This gives a dose of muscle extract of about 0.5 mg/ml minced tissue volume, which is approximately the minimum dose found to give maximum satellite cell proliferation with single myofibers in culture (Bischoff, 1986b).…”

Section: Muscle Extractmentioning

confidence: 94%

“…Previous studies have demonstrated the presence in crushed muscle extract of a growth factor that acts to commit quiescent satellite cells to enter the cell cycle (Bischoff, 1986b(Bischoff, , 1990b. The mitogenic activity is source-and target-cell specific and stimulates satellite cell proliferation in culture and in vivo when injected into muscle of newborn or adult rats (Bischoff, 198613).…”

Section: Augmented Mincementioning

confidence: 99%

“…Muscle extract was prepared from crushed leg and thigh muscles of 2-3-month-old rats as described (Bischoff, 1986b). As a control for tissue specificity, extract was also prepared from heart muscle.…”

Section: Muscle Extractmentioning

confidence: 99%

“…A number of environmental factors are thought to act to facilitate myogenesis during regeneration. There is evidence that quiescent satellite cells become committed to enter the cell cycle by growth factors released from injured myofibers (Kardami et al, 1985;Bischoff, 1986bBischoff, , 1990aChen and Quinn, 1992;Jimena et al, 1993;Mezzogiorno et al, 1993). Continued proliferation of the cells may be supported by mitogens such as fibroblast growth factor (FGF) and insulin-like growth factor (IGF), which are up-regulated in muscle after injury (Jennische and Olivecrona, 1987;Jennische, 1989;Guthridge et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of skeletal muscle regeneration

Bischoff

Heintz

1994

Developmental Dynamics

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161

103

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We have studied the effect of adding extra satellite cells or soluble factors from crushed muscle on regeneration of minced fragments from rat tibialis muscle. The muscle mince was wrapped in an artificial epimysium to prevent adhesions and cell immigration from adjacent muscles. Regeneration was quantitatively assessed by electrophoretic determination of the muscle-specific form of creatine kinase. Control minces exhibited three periods of change in creatine kinase activity during a 7-week regeneration period. Activity fell rapidly during the first week, then rose gradually from 1 3 weeks and increased more rapidly from 3-7 weeks. To augment the original complement of myogenic cells, satellite cells were isolated from the contralateral muscle, purified by density gradient centrifugation, and expanded in culture for 3 days before adding to the muscle mince. The added cells resulted in a 3-fold enhancement of creatine kinase activity throughout the regeneration period. Soluble muscle extract incorporated into a collagen matrix also stimulated regeneration when added to muscle mince. The extract accelerated the rate of creatine kinase increase during the 1 3 week period beyond that observed in the control or cell augmented mince, suggesting that factors in the extract may facilitate revascularization or reinnervation. The specific activity of creatine kinase was increased in regenerates augmented with both cells and extract, indicating that the effects enhance primarily myogenic processes.

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Section: Muscle Extractmentioning

confidence: 94%

Section: Augmented Mincementioning

confidence: 99%

Section: Muscle Extractmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhancement of skeletal muscle regeneration

Bischoff

Heintz

1994

Developmental Dynamics

Self Cite

161

103

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“…Second, using an elaborate biochemical fractionation protocol, purified myofiber extracts (PMEs) and interstitial extracts were concentrated and added to cultured satellite cells, or to a model for reversibly quiescent Pax7+ (RSCs) that arise after generation of differentiated myotubes following myoblast fusion. Skeletal muscle extracts were used in the past to identify FGF as a mitogen for satellite cell proliferation [11,12].…”

mentioning

confidence: 99%