A sandwich-type electrochemical immunosensor for carcinoembryonic antigen based on signal amplification strategy of optimized ferrocene functionalized Fe3O4@SiO2 as labels

Feng, Taotao; Qiao, Xiuwen; Wang, Haining; Sun, Zhao; Hong, Chenglin

doi:10.1016/j.bios.2015.11.001

Cited by 100 publications

(26 citation statements)

References 34 publications

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“…With this architecture, the authors obtained a linear range between 0.5 and 25 ng mL −1 and a LoD of 0.17 pg mL −1 . Still with a sandwich-type architecture, Feng et al [67] described a CEA immunosensor based on signal amplification using Fc-functionalized Fe 3 O/SiO 2 as labels and AuNP/GO as substrate. Anti-CEA were immobilized on both the substrate and the signaling NPs, the latter having catalytic properties towards H 2 O 2 oxidation (Figure 12).…”

Section: Discussionmentioning

confidence: 99%

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Recent Advances in Electrochemical Immunosensors

Piro

Reisberg

2017

Sensors

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Immunosensors have experienced a very significant growth in recent years, driven by the need for fast, sensitive, portable and easy-to-use devices to detect biomarkers for clinical diagnosis or to monitor organic pollutants in natural or industrial environments. Advances in the field of signal amplification using enzymatic reactions, nanomaterials such as carbon nanotubes, graphene and graphene derivatives, metallic nanoparticles (gold, silver, various oxides or metal complexes), or magnetic beads show how it is possible to improve collection, binding or transduction performances and reach the requirements for realistic clinical diagnostic or environmental control. This review presents these most recent advances; it focuses first on classical electrode substrates, then moves to carbon-based nanostructured ones including carbon nanotubes, graphene and other carbon materials, metal or metal-oxide nanoparticles, magnetic nanoparticles, dendrimers and, to finish, explore the use of ionic liquids. Analytical performances are systematically covered and compared, depending on the detection principle, but also from a chronological perspective, from 2012 to 2016 and early 2017.

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Section: Discussionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Recent Advances in Electrochemical Immunosensors

Piro

Reisberg

2017

Sensors

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“…In recent years, carbon-based matrices involving graphene oxide (GO) [ 28 ], reduced graphene oxide (rGO) [ 29 ], graphene sheets [ 30 , 31 , 32 ], carbon nanotubes [ 24 , 33 ] and their composites with nanoparticles [ 33 , 34 , 35 ], polymers [ 36 ], etc., have recently attracted much attention owing to their high conductivity, large surface area, and stability. In most of the cases, blocking of free surface areas on the sensor chip after capture molecule binding was achieved using BSA solution incubation.…”

Section: Matrix Selection Modification and Development Of Immunosmentioning

confidence: 99%

Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection

Arya

Estrela

2018

Sensors

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Electrochemical enzyme-linked immunosorbent assay (ELISA)-based immunoassays for cancer biomarker detection have recently attracted much interest owing to their higher sensitivity, amplification of signal, ease of handling, potential for automation and combination with miniaturized analytical systems, low cost and comparative simplicity for mass production. Their developments have considerably improved the sensitivity required for detection of low concentrations of cancer biomarkers present in bodily fluids in the early stages of the disease. Recently, various attempts have been made in their development and several methods and processes have been described for their development, amplification strategies and testing. The present review mainly focuses on the development of ELISA-based electrochemical immunosensors that may be utilized for cancer diagnosis, prognosis and therapy monitoring. Various fabrication methods and signal enhancement strategies utilized during the last few years for the development of ELISA-based electrochemical immunosensors are described.

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“…They are widely used in electromagnetics, the chemical industry, catalysis, sensors, acoustics, medicine, environmental protection and other related fields. In particular, Fe 3 O 4 NPs are widely used in the field of electrochemical immunosensors due to the excellent catalytic performance, impeccable magnetism, good biocompatibility and large specific surface area [31][32][33]. Prussian blue has great potential in many fields and has been widely used in electrocatalysis, biosensors, chemical sensors, rechargeable batteries and electroanalytical chemistry [34].…”

Section: Introductionmentioning

confidence: 99%

A Label-Free Immunosensor Based on Graphene Oxide/Fe3O4/Prussian Blue Nanocomposites for the Electrochemical Determination of HBsAg

et al. 2020

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In this article, a highly sensitive label-free immunosensor based on a graphene oxide (GO)/Fe3O4/Prussian blue (PB) nanocomposite modified electrode was developed for the determination of human hepatitis B surface antigen (HBsAg). In this electrochemical immunoassay system, PB was used as a redox probe, while GO/Fe3O4/PB nanocomposites and AuNPs were prepared and coated on screen-printed electrodes to enhance the detection sensitivity and to immobilize the hepatitis B surface antibody (HBsAb). The immunosensor was fabricated based on the principle that the decrease in peak currents of PB is proportional to the concentration of HBsAg captured on the modified immunosensor. The experimental results revealed that the immunosensor exhibited a sensitive response to HBsAg in the range of 0.5 pg·mL−1 to 200 ng·mL−1, and with a low detection limit of 0.166 pg·mL−1 (S/N = 3). Furthermore, the proposed immunosensor was used to detect several clinical serum samples with acceptable results, and it also showed good reproducibility, selectivity and stability, which may have a promising potential application in clinical immunoassays.

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A sandwich-type electrochemical immunosensor for carcinoembryonic antigen based on signal amplification strategy of optimized ferrocene functionalized Fe3O4@SiO2 as labels

Cited by 100 publications

References 34 publications

Recent Advances in Electrochemical Immunosensors

Recent Advances in Electrochemical Immunosensors

Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection

A Label-Free Immunosensor Based on Graphene Oxide/Fe3O4/Prussian Blue Nanocomposites for the Electrochemical Determination of HBsAg

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