A sandwich-type bacteriophage-based amperometric biosensor for the detection of Shiga toxin-producing <i>Escherichia coli</i> serogroups in complex matrices

Quintela, Irwin A.; Wu, Vivian C.H.

doi:10.1039/d0ra06223e

Cited by 14 publications

(8 citation statements)

References 55 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sensor was highly specific to various Shiga toxin-producing E. coli (STEC) serogroups. The amperometric biosensor showed a detection limit of 10–10 2 CFU/mL for the STEC O26, O157, and O179 strains within 1 h [ 15 ]. In another study, Nikkhoo et al introduced a quick and inexpensive bacterial detection platform using T6 bacteriophages in combination with ion-selective field-effect transistors (ISFETs) and potassium-sensitive membranes (potassium ion detection).…”

Section: Types Of Phage-based Biosensorsmentioning

confidence: 99%

“…The development of phage-based biosensors as a tool for the direct detection of live pathogens in food is an important and attractive approach [ 14 ]. Presently, several phage-based biosensors have been developed that incorporate various transducers, including electrochemical [ 15 ], quartz crystal microbalance (QCM) [ 16 ], surface plasmon resonance (SPR) [ 17 ], magnetoelastic (ME) [ 18 ], and others. Most of these biosensors have been designed using the whole/intact phage or the phage proteins as well as the cytoplasmic markers that are released following the phage infection.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment

et al. 2022

View full text Add to dashboard Cite

Foodborne microorganisms are an important cause of human illness worldwide. Two-thirds of human foodborne diseases are caused by bacterial pathogens throughout the globe, especially in developing nations. Despite enormous developments in conventional foodborne pathogen detection methods, progress is limited by the assay complexity and a prolonged time-to-result. The specificity and sensitivity of assays for live pathogen detection may also depend on the nature of the samples being analyzed and the immunological or molecular reagents used. Bacteriophage-based biosensors offer several benefits, including specificity to their host organism, the detection of only live pathogens, and resistance to extreme environmental factors such as organic solvents, high temperatures, and a wide pH range. Phage-based biosensors are receiving increasing attention owing to their high degree of accuracy, specificity, and reduced assay times. These characteristics, coupled with their abundant supply, make phages a novel bio-recognition molecule in assay development, including biosensors for the detection of foodborne bacterial pathogens to ensure food safety. This review provides comprehensive information about the different types of phage-based biosensor platforms, such as magnetoelastic sensors, quartz crystal microbalance, and electrochemical and surface plasmon resonance for the detection of several foodborne bacterial pathogens from various representative food matrices and environmental samples.

show abstract

Section: Types Of Phage-based Biosensorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The Nanoluc reporter was fused with a crystalline-specific carbohydrate binding module. This novel work was able to concentrate the fusion reporter and achieve a LOD of <10 CFU/ml in 3 h. Quintela and Wu (2020) employed bacteriophages in a sandwich-type amperometric biosensor for the detection of STEC O26, O157, and O179 strains in complex matrices. Environmentally isolated bacteriophages were modified and directly immobilized onto a streptavidin-coated SPCE.…”

Section: Bacteriophage-based Biosensorsmentioning

confidence: 99%

Advances, applications, and limitations of portable and rapid detection technologies for routinely encountered foodborne pathogens

et al. 2022

Self Cite

View full text Add to dashboard Cite

Traditional foodborne pathogen detection methods are highly dependent on pre-treatment of samples and selective microbiological plating to reliably screen target microorganisms. Inherent limitations of conventional methods include longer turnaround time and high costs, use of bulky equipment, and the need for trained staff in centralized laboratory settings. Researchers have developed stable, reliable, sensitive, and selective, rapid foodborne pathogens detection assays to work around these limitations. Recent advances in rapid diagnostic technologies have shifted to on-site testing, which offers flexibility and ease-of-use, a significant improvement from traditional methods’ rigid and cumbersome steps. This comprehensive review aims to thoroughly discuss the recent advances, applications, and limitations of portable and rapid biosensors for routinely encountered foodborne pathogens. It discusses the major differences between biosensing systems based on the molecular interactions of target analytes and biorecognition agents. Though detection limits and costs still need further improvement, reviewed technologies have high potential to assist the food industry in the on-site detection of biological hazards such as foodborne pathogens and toxins to maintain safe and healthy foods. Finally, this review offers targeted recommendations for future development and commercialization of diagnostic technologies specifically for emerging and re-emerging foodborne pathogens.

show abstract

“…However, the complexity of environmental samples requires parallel detection methods for multiple targets to increase the breadth, sensitivity, and specificity of the detection of significant pathogens. Novel capture elements, such as bacteriophages integrated into an electrochemical-based detection system, can successfully capture STEC strains in surface water (Quintela and Wu, 2020).…”

Section: Improved Sampling and Pathogen Detectionmentioning

confidence: 99%

Shiga toxin-producing Escherichia coli outbreaks in California’s leafy greens production continuum

Lacombe

Quintela

Liao

et al. 2022

Front. Food. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

Despite efforts to control pathogenic hazards in agriculture, leafy greens grown in California were the source of several high-profile outbreaks of Shiga toxin-producing E. coli (STEC). The U.S. Food and Drug Administration (FDA) analysis of the outbreaks found three reoccurring patterns with leafy greens contaminated with STEC, specifically E. coli O 157:H7, in 2018–2020: the presence of pathogenic E. coli, common geographical regions, and issues with activities on adjacent lands, such as cattle production and migratory birds. The FDA’s response to the recurring outbreaks associated with leafy greens is the Leafy Greens STEC Action Plan (LGAP). In partnership with the U.S. Environmental Protection Agency (EPA), a regulatory pathway was created for the approval of commercial sanitizers that can be applied to agricultural irrigation water to combat STEC, specifically E. coli O 157:H7. However, the protocol has several real-world limitations and economic consequences, such as the potential to overuse sanitizing products, thus adding disinfection by-products classified as pollutants. In addition, there have been several initiatives due to systems research on the local, state, and federal levels to provide technical assistance for the further improvement of Good Agricultural Practices (GAPs). This review considers the factors involved in leafy green production, such as agricultural water, climate change, and adjacent land use, contributing to increased susceptibility to pathogens contamination and how the implementation of sanitizers impacts food safety. The review discusses potential future improvements to agricultural water safety and quality in the context of improving food safety.

show abstract

A sandwich-type bacteriophage-based amperometric biosensor for the detection of Shiga toxin-producing Escherichia coli serogroups in complex matrices

Abstract: Sandwich-type bacteriophage-based amperometric biosensor for the detection of Shiga toxin-producing Escherichia coli serogroups in complex matrices.

Cited by 14 publications

References 55 publications

Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment

Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment

Advances, applications, and limitations of portable and rapid detection technologies for routinely encountered foodborne pathogens

Shiga toxin-producing Escherichia coli outbreaks in California’s leafy greens production continuum

Contact Info

Product

Resources

About