Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment

Al-Hindi, Rashad R.; Alharbi, Mona G.; Alotibi, Ibrahim; Azhari, Sheren; Qadri, Ishtiaq; Alamri, Turki; Harakeh, Steve; Applegate, Bruce; Bhunia, Arun K.

doi:10.3390/bios12100905

Cited by 26 publications

(10 citation statements)

References 141 publications

(141 reference statements)

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“…Tail proteins of bacteriophage viruses help in specific and efficient recognition of bacteria, making it apt as a biosensor. The major advantages, such as robustness, highly sensitive and selective binding and low-cost preparation, make it a popular bio-recognition element (Al-Hindi et al , 2022). The various biosensors have been developed with time.…”

Section: Classification Of Biosensormentioning

confidence: 99%

Application of biosensors against food-borne pathogens

Singh,

Sharanagat

2023

NFS

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Purpose Nature and occurrence of food-borne pathogens in raw and processed food products evolved greatly in the past few years due to new modes of transmission and resistance build-up against sundry micro-/macro-environmental conditions. Assurance of food health and safety thus gained immense importance, for which bio-sensing technology proved very promising in the detection and quantification of food-borne pathogens. Considering the importance, different studies have been performed, and different biosensors have been developed. This study aims to summarize the different biosensors used for the deduction of food-borne pathogens. Design/methodology/approach The present review highlights different biosensors developed apropos to food matrices, factors governing their selection, their potential and applicability. The paper discusses some related key challenges and constraints and also focuses on the needs and future research prospects in this field. Findings The shift in consumers’ and industries’ perceptions directed the further approach to achieve portable, user and environmental friendly biosensing techniques. Despite of these developments, it was still observed that the comparison among the different biosensors and their categories proved tedious on a single platform; since the food matrices tested, pathogen detected or diagnosed, time of detection, etc., varied greatly and very few products have been commercially launched. Conclusively, a challenge lies in front of food scientists and researchers to maintain pace and develop techniques for efficiently catering to the needs of the food industry. Research limitations/implications Biosensors deduction limit varied with the food matrix, type of organism, material of biosensors’ surface, etc. The food matrix itself consists of complex substances, and various types of food are available in nature. Considering the diversity of food there is a need to develop a universal biosensor that can be used for all the food matrices for a pathogen. Further research is needed to develop a pathogen-specific biosensor that can be used for all the food products that may have accuracy to eliminate the traditional method of deduction. Originality/value The present paper summarized and categorized the different types of biosensors developed for food-borne pathogens. Graphical abstract

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Section: Classification Of Biosensormentioning

confidence: 99%

Application of biosensors against food-borne pathogens

Singh,

Sharanagat

2023

NFS

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“…To expedite the diagnostic workflow for faster results (less than 24 h), enrichment broths are routinely tested by a pathogen-specific PCR assay or antibody-based lateral flow immunochromatographic assays ( Urusov et al., 2019 ; Huang et al., 2020b ; Fogaça et al., 2021 ). Bacteriophage-based pathogen detection is also gaining significant interest among microbiologists ( Al-Hindi et al., 2022 ). However; most clinical laboratory diagnostic approaches rely on isolated individual colonies, which are achieved by direct plating of clinical samples on Petri plates.…”

Section: Bardot-based Pathogen Detection Approaches For Individual Pa...mentioning

confidence: 99%

“…Modern-day nano-bio sensors have begun to revolutionize the detection of a single molecule or a single cell with high precision, but most depend on probes (labels) or signature tags for the identification of targets ( Bhunia, 2014 ; Zhang et al., 2016 ; Ali et al., 2020 ; Nayl et al., 2020 ; Xu et al., 2021 ; Al-Hindi et al., 2022 ). Very few biosensors operate independently of molecular probes, but are reliant on a database, and are categorized as label-free.…”

Section: Introductionmentioning

confidence: 99%

Petri-plate, bacteria, and laser optical scattering sensor

Bhunia

Singh

Parker

et al. 2022

Front. Cell. Infect. Microbiol.

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Classical microbiology has paved the path forward for the development of modern biotechnology and microbial biosensing platforms. Microbial culturing and isolation using the Petri plate revolutionized the field of microbiology. In 1887, Julius Richard Petri invented possibly the most important tool in microbiology, the Petri plate, which continues to have a profound impact not only on reliably isolating, identifying, and studying microorganisms but also manipulating a microbe to study gene expression, virulence properties, antibiotic resistance, and production of drugs, enzymes, and foods. Before the recent advances in gene sequencing, microbial identification for diagnosis relied upon the hierarchal testing of a pure culture isolate. Direct detection and identification of isolated bacterial colonies on a Petri plate with a sensing device has the potential for revolutionizing further development in microbiology including gene sequencing, pathogenicity study, antibiotic susceptibility testing , and for characterizing industrially beneficial traits. An optical scattering sensor designated BARDOT (bacterial rapid detection using optical scattering technology) that uses a red-diode laser, developed at the beginning of the 21st century at Purdue University, some 220 years after the Petri-plate discovery can identify and study bacteria directly on the plate as a diagnostic tool akin to Raman scattering and hyperspectral imaging systems for application in clinical and food microbiology laboratories.

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“…In particular, in developing countries, two-thirds of human foodborne diseases are caused by bacterial pathogens. 1 The annual cost of foodborne illnesses in the USA exceeds $50 billion, and more than 23 million cases and more than 4700 deaths related to foodborne diseases occur in Europe every year, having a major impact on society. 2 The main foodborne pathogenic bacteria include Escherichia coli , Listeria monocytogenes , Staphylococcus aureus , and Salmonella sp.…”

Section: Introductionmentioning

confidence: 99%