A sandwich enzyme-linked immunosorbent assay for the quantitation of human plasma ferritin

Bleicher, Andie; Unger, Holger W.; Rogerson, Stephen J.; Aitken, Elizabeth H.

doi:10.1016/j.mex.2018.06.010

Cited by 11 publications

(7 citation statements)

References 5 publications

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“…Before concluding this section, it should be noted that numerous commercial optical products exist to qualitatively and quantitatively measure the concentrations of target analytes. These methods include colorimetric assays, fluorometric assays, chemiluminescence assays, and various forms of ELISA kits. − Some commonly encountered challenges among commercial assays are the need for expensive kits and tedious sample preparation for labeling . The exceptional optical properties of 2D materials (such as tunable band structure as discussed previously) offer opportunities for their utilization in ultrasensitive, tunable optical biosensors with wide dynamic range, as detailed elsewhere …”

Section: Biosensing and Biomedical Applicationsmentioning

confidence: 99%

Two-Dimensional Materials in Biosensing and Healthcare: FromIn VitroDiagnostics to Optogenetics and Beyond

et al. 2019

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Since the isolation of graphene in 2004, there has been an exponentially growing number of reports on layered two-dimensional (2D) materials for applications ranging from protective coatings to biochemical sensing. Due to the exceptional, and often tunable, electrical, optical, electrochemical, and physical properties of these materials, they can serve as the active sensing element or a supporting substrate for diverse healthcare applications. In this review, we provide a survey of the recent reports on the applications of 2D materials in biosensing and other emerging healthcare areas, ranging from wearable technologies to optogenetics to neural interfacing. Specifically, this review provides (i) a holistic evaluation of relevant material properties across a wide range of 2D systems, (ii) a comparison of 2D material-based biosensors to the state-of-the-art, (iii) relevant material synthesis approaches specifically reported for healthcare applications, and (iv) the technological considerations to facilitate mass production and commercialization.

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Section: Biosensing and Biomedical Applicationsmentioning

confidence: 99%

Two-Dimensional Materials in Biosensing and Healthcare: FromIn VitroDiagnostics to Optogenetics and Beyond

et al. 2019

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“…It has to be noted that the concentrations that were chosen for each protein were modified based on the literature, and correspond to the amounts that can be found both in the cell culture media and in the cerebrospinal fluid. [19]…”

Section: Resultsmentioning

confidence: 99%

Cell Membrane‐Coated Magnetic Nanocubes with a Homotypic Targeting Ability Increase Intracellular Temperature due to ROS Scavenging and Act as a Versatile Theranostic System for Glioblastoma Multiforme

Tapeinos

Tomatis

Battaglini

et al. 2019

Adv Healthcare Materials

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In this study, hybrid nanocubes composed of magnetite (Fe 3 O 4 ) and manganese dioxide (MnO 2 ), coated with U-251 MG cell-derived membranes (CM-NCubes) are synthesized. The CM-NCubes demonstrate a concentration-dependent oxygen generation (up to 15%), and, for the first time in the literature, an intracellular increase of temperature (6 °C) due to the exothermic scavenging reaction of hydrogen peroxide (H 2 O 2 ) is showed. Internalization studies demonstrate that the CM-NCubes are internalized much faster and at a higher extent by the homotypic U-251 MG cell line compared to other cerebral cell lines. The ability of the CM-NCubes to cross an in vitro model of the blood-brain barrier is also assessed. The CM-NCubes show the ability to respond to a static magnet and to accumulate in cells even under flowing conditions. Moreover, it is demonstrated that 500 µg mL −1 of sorafenib-loaded or unloaded CM-NCubes are able to induce cell death by apoptosis in U-251 MG spheroids that are used as a tumor model, after their exposure to an alternating magnetic field (AMF). Finally, it is shown that the combination of sorafenib and AMF induces a higher enzymatic activity of caspase 3 and caspase 9, probably due to an increment in reactive oxygen species by means of hyperthermia. Cell Membrane NanocubesThe ORCID identification number(s) for the author(s) of this article can be found under https://doi.

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“…This competitive detection method resulted in a detection range between 2.5–350 ng/mL [ 45 ]. Bleicher et al reported a sandwich ELISA using polyclonal antibodies for the quantification of ferritin with a detection range between 3.2–232 ng/mL [ 46 ]. Garg et al reported microfluidics-based nanoparticle-enhanced electrochemical detection by using an integrated electrochemically active screen-printed electrode (SPE) to monitor SF with a detection range between 7.81–500 ng/mL [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Serum Ferritin-Specific Nanobodies and Development towards a Diagnostic Immunoassay

Lin

Wang

et al. 2022

Biomolecules

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Serum ferritin (SF) is an iron-rich protein tightly connected with iron homeostasis, and the variations are frequently observed in diseased states, including iron-deficiency anemia, inflammation, liver disease, and tumors, which renders SF level an indicator of potential malignancies in clinical practice. Nanobodies (Nbs) have been widely explored and developed into theranostic reagents. Surprisingly, no reports stated the identification of anti-SF Nbs, nor the potential of such Nbs as a diagnostic tool. In this study, we generated SF-specific Nbs and provided novel clinical diagnostic approaches to develop an immunoassay. An immune library was constructed after immunizing an alpaca with SF, and five Nbs specifically targeting human SF were retrieved. The obtained Nbs exhibited robust properties including high stability, affinity, and specificity. Then, an ELISA-based test using a heterologous Nb-pair was developed. The calibration curve demonstrated a linear range of SF between 9.0 to 1100 ng/mL, and a limit of detection (LOD) of 1.01 ng/mL. The detecting recovery and coefficient variation (CV) were determined by spiking different concentrations of SF into the serum sample, to verify the successful application of our selected Nbs for SF monitoring. In general, this study generated SF-specific Nbs and demonstrated their potential as diagnostic immunoassay tools.

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A sandwich enzyme-linked immunosorbent assay for the quantitation of human plasma ferritin

Abstract: Graphical abstract

Cited by 11 publications

References 5 publications

Two-Dimensional Materials in Biosensing and Healthcare: FromIn VitroDiagnostics to Optogenetics and Beyond

Two-Dimensional Materials in Biosensing and Healthcare: FromIn VitroDiagnostics to Optogenetics and Beyond

Cell Membrane‐Coated Magnetic Nanocubes with a Homotypic Targeting Ability Increase Intracellular Temperature due to ROS Scavenging and Act as a Versatile Theranostic System for Glioblastoma Multiforme

Identification of Serum Ferritin-Specific Nanobodies and Development towards a Diagnostic Immunoassay

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