A SAGE-based comparison between glomerular and aortic endothelial cells

Abstract

. A SAGEbased comparison between glomerular and aortic endothelial cells.

VEGF regulation of endothelial nitric oxide synthase in glomerular endothelial cells

“…These observations and reports in the literature suggest that data on eNOS regulation cannot be extrapolated from one endothelial cell type to another. Recent microarray analysis supports this notion; GENCs from bovine kidney possess a distinct gene expression profile compared to nonrenal endothelial cells [31]. Since phosphorylation of eNOS on ser1177 is critical for activation of the enzyme, convergence of two signaling pathways (MAPK and PI3K) on this residue may have dual significance.…”

Phenotypic Heterogeneity of the Endothelium

“…65,131 In some cases, ECs have been rapidly sorted from different blood vessel types (in health and disease) and assayed for gene expression by serial analysis of gene expression (SAGE), subtractive hybridization, or DNA microarray analysis. [132][133][134][135][136][137][138] However, even with rapid harvest times, ECs may undergo phenotypic drift (examples are found elsewhere 139,140 ). A technique that holds promise for the future is laser capture microdissection of ECs from intact blood vessels, followed by RT-PCR and/or DNA microarray analysis.…”

Endothelial molecules decipher the mechanisms and functional pathways in antibody-mediated rejection

“…We identified a literature based ''endothelial cell-associated transcript'' (ENDAT) set (323 probe sets; 119 unique genes), in which genes were identified based on their selective expression in cultured human endothelial cells when compared with non-endothelial cells or by serial analysis gene expression (SAGE) libraries comparing tag frequencies in endothelial versus non-endothelial libraries [49,50]. In a large number of kidney transplant biopsies for clinical indications, the biopsies with high expression of ENDATs and HLA antibodies showed histopathological lesions of ABMR (transplant glomerulopathy, capillaritis, glomerulitis, peritubular capillary basement membrane multilayering, and fibrosis/atrophy) and poor outcomes in comparison to biopsies with HLA antibodies and no ENDAT upregulation [51].…”