A RP-HPLC method for the determination of tea catechins

Khokhar, S.; Venema, D.P.; Hollman, P.C.H.; Dekker, Matthijs; Jongen, W.M.F.

doi:10.1016/s0304-3835(97)04653-3

Cited by 84 publications

(58 citation statements)

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“…In particular, method performance parameters, evaluated on the basis of a rigorous validation protocol, revealed lower LODs and LOQs when compared with previous methods [3,34], a better resolution between analytes [16,17,19,[20][21][22] shorter analysis times [16,18], thus minimizing the analysis cost. Furthermore, it also demonstrated good selectivity and accuracy, making it possible to quantify flavan-3-ols without any sample pretreatment and in spite of the complexity of the polyphenolic fraction and the possible presence of other interfering ingredients.…”

Section: Discussionmentioning

confidence: 94%

Identification, quantitation, and method validation for flavan‐3‐ols in fermented ready‐to‐drink teas from the Italian market using HPLC‐UV/DAD and LC‐MS/MS

Cordero

Canale

Rio

et al. 2009

J of Separation Science

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The present study is focused on flavan-3-ols characterizing the antioxidant properties of fermented tea (Camellia sinensis). These bioactive compounds, object of nutritional claims in commercial products, should be quantified with rigorous analytical procedures whose accuracy and precision have been stated with a certain level of confidence. An HPLC-UV/ DAD method, able to detect and quantify flavan-3-ols in infusions and ready-to-drink teas, has been developed for routine analysis and validated by characterizing several performance parameters. The accuracy assessment has been run through a series of LC-MS/MS analyses. Epigallocatechin, (1)-catechin, (À)-epigallocatechingallate, (À)-epicatechin, (À)-gallocatechingallate, (À)-epicatechingallate, and (À)-catechingallate were chosen as markers of the polyphenolic fraction. Quantitative results showed that samples obtained from tea leaves infusion were richer in polyphenolic antioxidants than those obtained through other industrial processes. The influence of shelf-life and packaging material on the flavan-3-ols content was also considered; markers decreased, with an exponential trend, as a function of time within the shelf life while packaging materials demonstrated to influence differently the flavan-3-ol fraction composition over time. The method presented here provides quantitative results with a certain level of confidence and is suitable for a routine quality control of iced teas whose antioxidant properties are object of nutritional claim.

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Section: Discussionmentioning

confidence: 94%

Identification, quantitation, and method validation for flavan‐3‐ols in fermented ready‐to‐drink teas from the Italian market using HPLC‐UV/DAD and LC‐MS/MS

Cordero

Canale

Rio

et al. 2009

J of Separation Science

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“…More and more bioactive ingredients are being extracted from natural materials. However, a few traditional techniques can be employed for selectively recovering the ingredients with similar molecular structure, for example (À)-epigallocatechin, (1)-catechin, (À)-epicatechin, (À)-epigallocatechingallate, and (À)-epicatechingallate in green tea (Khokhar et al 1997). Molecular imprinting media are prepared in the presence of a template; this results in the formation of specific recognition cavities complementary to the template in shape and chemical functionality (Vallano and Remcho 2000).…”

Section: Introductionmentioning

confidence: 99%

Characterization and selectivity studies of molecular imprinted membranes of Puerarin using scanning electron microscopy

Qin¹,

Yang²,

Karim³

2011

Scanning

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Molecular-imprinted membranes of Puerarin were prepared by phase inversion technique with acrylonitrile-acrylic acid copolymer (P (AN-co-AA)). To characterize P (AN-co-AA), ubbelohde viscometer was used to measure its viscosity-molecular weight. P (AN-co-AA) with different molecular weight was used to prepare membranes. The copolymer-dimethyl sulfoxide solution with Puerarin (PU) template was coagulated in water at various temperatures. The increase in P (AN-co-AA) molecular weight and the decrease in coagulation temperature caused an increase in PU recognition property of the resultant membrane. The PU imprinted membrane prepared with P (AN-co-AA) showed good selective ability to PU. The purity of PU increased from 56.51 to 98.41 wt%. Surface and cross-section morphology of the membranes were analyzed by using scanning electron micrograph. High-performance liquid chromatography was used for the quantification of Puerarin in isolated fraction.

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“…[1][2][3][4][5][6][7][8][9] High-performance liquid chromatography and capillary electrophoresis with ultraviolet detection are the most cited techniques for separation, identification and quantitation. However, mass spectrometry, and electrochemical, fluorescence and chemiluminescence detection are also used in cases where more sensitive or selective detection is needed.…”

Section: Introductionmentioning

confidence: 99%

“…Analytical techniques, such as gas chromatography, spectrophotometry, nuclear magnetic resonance spectroscopy and biosensors have also been used for the determination of catechin. [1][2][3][4][5][6][7][8][9] The development of functional models of metalloenzymes for catalyst oxidation reactions is a subject of great interest. [10][11][12][13][14][15][16] Neves et al [17][18][19][20][21][22][23][24][25] have extensively synthesized and characterized model complexes to mimic the active site of the different enzymes (e.g., catechol oxidase, peroxidase, galactose oxidase, catalase, purple acid phosphatase).…”

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Determination of catechin in green tea using a catechol oxidase biomimetic sensor

Fernandes

Osório

Anjos

et al. 2008

J. Braz. Chem. Soc.

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Um sensor biomimético catecol oxidase, baseado em um novo complexo cobre(II) foi desenvolvido para determinação de catequina em chá verde e os resultados comparados com os obtidos por eletroforese capilar. O complexo dinuclear de cobre(II) [Cu 2 (HL)(µ-CH 3 COO)] (ClO 4 ), contendo o ligante N,N-[bis-(2-piridilmetil)]-N',N'-[(2-hidroxibenzil)(2-hidroxi-3,5-di-tert-butilbenzil)]-1,3-propanodiamino-2-ol (H 3 L), foi sintetizado e caracterizado por IV, 1 H RMN e análise elementar. As melhores condições para otimização do sensor biomimético foram estabelecidas por voltametria de onda quadrada. O melhor desempenho desse sensor foi obtido em 75:15:10% (m/m/m) de pó de grafite:nujol:complexo de cobre(II), tampão fosfato 0,05 mol L -1 (pH 7,5) e freqüência, amplitude de potencial, incremento em 30 Hz, 80 mV, 3,3 mV, respectivamente. A curva analítica foi linear na faixa de concentração 4,95 × 10 -6 a 3,27 × 10 -5 mol L -1 (r=0,9993) com limite de detecção de 2,8 × 10 -7 mol L -1 . Esse sensor biomimético demonstrou longo tempo de estabilidade (9 meses; 800 determinações) e reprodutibilidade com um desvio padrão relativo de 3,5%. A recuperação da catequina em amostras de chá verde variou de 93,8 a 106,9% e a determinação, comparada com a obtida usando eletroforese capilar, mostrou-se aceitável a um nível de confiança de 95%.A catechol oxidase biomimetic sensor, based on a novel copper(II) complex, was developed for the determination of catechin in green tea and the results were compared with those obtained by capillary electrophoresis. The dinuclear copper(II) complex, [Cu 2 (HL)(µ-CH 3 COO)](ClO 4 ), containing the ligand N,N-[bis-(2-pyridylmethyl)]-N',N'-[(2-hydroxybenzyl)(2-hydroxy-3,5-ditert-butylbenzyl)]-1,3-propanediamine-2-ol (H 3 L), was synthesized and characterized by IR, 1 H NMR and elemental analysis. The best conditions for the optimization of the biomimetic sensor were established by square wave voltammetry. The best performance for this sensor was obtained in 75:15:10% (m/m/m) of the graphite powder:nujol:copper(II) complex, 0.05 mol L -1 phosphate buffer solution (pH 7.5) and frequency, pulse amplitude, scan increment at 30 Hz, 80 mV, 3.3 mV, respectively. The analytical curve was linear in the concentration range 4.95 × 10 -6 to 3.27 × 10 -5 mol L -1 (r = 0.9993) with a detection limit of 2.8 × 10 -7 mol L -1 . This biomimetic sensor demonstrated long-term stability (9 months; 800 determinations) and reproducibility with a relative standard deviation of 3.5%. The recovery of catechin from green tea samples ranged from 93.8 to 106.9% and the determination, compared with that obtained using capillary electrophoresis, was found to be acceptable at the 95% confidence level.Keywords: catechin, green tea, biomimetic sensor, square wave voltammetry IntroductionTea is one of the most consumed beverages in the world and was firstly used in China for its medicinal properties 5000 years ago. Recent epidemiological studies have shown that the consumption of tea may help to prevent cancers in humans because the tea l...

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A RP-HPLC method for the determination of tea catechins

Cited by 84 publications

References 1 publication

Identification, quantitation, and method validation for flavan‐3‐ols in fermented ready‐to‐drink teas from the Italian market using HPLC‐UV/DAD and LC‐MS/MS

Identification, quantitation, and method validation for flavan‐3‐ols in fermented ready‐to‐drink teas from the Italian market using HPLC‐UV/DAD and LC‐MS/MS

Characterization and selectivity studies of molecular imprinted membranes of Puerarin using scanning electron microscopy

Determination of catechin in green tea using a catechol oxidase biomimetic sensor

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