A roundabout strategy for high-purity glycolic acid biopreparation via a resting cell bio-oxidation catalysis of ethylene glycol

Abstract

High-purified glycolic acid (GA) is necessary for the blocking unit of medical materials that provides a promising value-added utilization for ethylene glycol (EG). Currently, almost all commercial process for GA...

Improvement in Ethylene Glycol Bioconversion to Glycolic Acid by Gluconobacter oxydans Using Central Composite Experimental Design and Bioreactor Fed-Batch Strategies

“…This method is known for its high efficiency and continuous operation capability but requires regeneration of adsorbents, potentially increasing operating costs. Electrodialysis [35,36] uses a selective membrane and an electric field to separate ions, remove impurities and obtain a purer GA. This method is efficient and sustainable, but can be expensive due to the cost of membranes and the energy required.…”

An Effective Method for the Direct Crystallization of Xylonic Acid from Fermentation Broth of Agricultural Residue Hydrolysate

“…And when it comes to purification of products, the XA crystallization in aqueous phase system is still an unattainable goal, mainly because hydrocolloids unique stability. Additionally, Gluconobacter oxydans possesses promiscuous oxidizing enzymes, which can convert xylose and many sugars and even sugar alcohols present in their specific acids, various chemical components in the fermentation broth making difficult the downstream processes for purification [18][19][20][21] . Xylonates purified by activated carbon adsorption or membrane separation are still contaminated by the culture medium and cell metabolites in the fermentation broth, making it difficult to achieve high purity 6 goal.…”

“…The cell density was equivalent to OD 600 = 4. A refrigerated centrifuge (AvantiJ-26XP, Beckman Coulter, USA) was used to separate fermentation broth and harvest cells at 5683 g for 5 min 18 . The fermentation pH value was adjusted to around 5.5 by the neutralizer addition with 50 % KOH and This preprint research paper has not been peer reviewed.…”

A hybrid deep learning framework driven by data and reaction mechanism for predicting sustainable glycolic acid production performance

“…This complex is difficult to remove. In addition, GA is easy to self‐polymerize into low molecular weight glycolic acid dimer, glycolic acid tetramer, and other by‐products 28 . These characteristics of glycolic acid are difficult to purify, which will directly lead to the low purity of GA crystal 29 .…”

“…In addition, GA is easy to self-polymerize into low molecular weight glycolic acid dimer, glycolic acid tetramer, and other byproducts. 28 These characteristics of glycolic acid are difficult to purify, which will directly lead to the low purity of GA crystal. 29 If the purpose is to synthesize high-purity GA crystals, this will put forward high requirements for the synthesis and separation process of GA.…”