A role played by the vitelline diverticulum in the yolk sac resorption in young post-hatched chickens

Esteban, Susana; Rayó, J. M.; Moreno, María Alejandra; Sastre, Magdalena; Rial, Rubén V.; Tur, Juan

doi:10.1007/bf00571262

Cited by 45 publications

(24 citation statements)

References 10 publications

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“…Although previous studies recorded an accelerated consumption of the yolk sac toward hatch (Yadgary et al, 2010) and posthatch (Noy and Sklan, 1999), the observed decrease in YSM mRNA levels may be explained by the transfer of yolk nutrients directly from the yolk sac into the intestine (Esteban et al, 1991;Sulaiman et al, 1996) and not just thorough the YSM (Lambson, 1970;Noble and Cocchi, 1990); Noy and Sklan (2001) indicated that from the day of hatch to d 4 posthatch, the major route of yolk utilization is via the yolk stalk into the small intestine. It is possible that the shift from the route through the YSM to the route through the intestine occurs as early as 20E to 21E; thus, nutrient transport and digestive mechanisms are downregulated in the YSM during this period.…”

Section: Resultsmentioning

confidence: 80%

The chick embryo yolk sac membrane expresses nutrient transporter and digestive enzyme genes

2011

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The yolk sac membrane plays a major role in the transport of nutrients from the yolk contents to the chick embryo. We examined whether the yolk sac membrane expresses genes for nutrient digestion, enzymes, and nutrient transporters. We evaluated relative mRNA abundance of the digestive enzymes aminopeptidase N (APN) and sucrase-isomaltase (SI); the nutrient transporters oligopeptide transporter Pept1, cationic amino acid transporter CAT1, and sodium glucose transporter SGLT1; and the micronutrient transporters type IIb sodium phosphate cotransporter NPT2b, calcium transporter TRPV6, and zinc transporter ZnT-1 from embryonic d 11 (11E) to 21E (day of hatch) by real-time reverse-transcription PCR. The yolk sac membrane expressed all the examined genes, which exhibited several patterns of expression. Relative abundance of APN mRNA increased in the yolk sac membrane from 11E to 17E and decreased from 17E to 20E. Expression of PepT1 increased from 11E to 15E and decreased from 15E to 20E. In contrast, CAT1 expression decreased from 11E to 13E and increased from 15E to 17E. Expression of SGLT1 increased between 15E and 20E and decreased substantially between 20E and 21E. Expression of NPT2b increased during incubation and exhibited the highest relative expression of all the examined genes, particularly on 20E to 21E. Expression of TRPV6 decreased from 11E to 13E and increased substantially from 15E to 19E. No significant difference was found between the sampled days for ZnT-1 or SI expression, with the latter exhibiting the lowest relative expression of all the genes studied. These results present the first documentation of nutrient transporter and digestive enzyme gene-expression patterns in the yolk sac membrane, and provide a basis for future research on the capacity of the yolk sac membrane for nutrient digestion and transport.

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Section: Resultsmentioning

confidence: 80%

The chick embryo yolk sac membrane expresses nutrient transporter and digestive enzyme genes

2011

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“…Therefore, it is not very surprising to find studies commonly using inoculum doses in the range of 10 6 cfu or more to infect chicks a few days old (Bjerrum et al, 2003;Santos et al, 2008;Vandeplas et al, 2009). Nevertheless, the results of this study show that this maternal protection lasts for only a few days because it is already drastically reduced at 9 d of age, probably because of the rapid resorption of vitelline contents (Esteban et al, 1991). From the third week of age, the birds are able to develop their own mucosal immune defenses in the gut, which interfere with the colonization and persistence of the pathogen organism inoculated.…”

Section: Discussionmentioning

confidence: 94%

Salmonella Typhimurium oral challenge model in mature broilers: Bacteriological, immunological, and growth performance aspects

et al. 2011

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In this study, Salmonella enterica serovar Typhimurium challenge models were tested to identify the best conditions under which to perform the experimental infection of 3-wk-old broilers. Such a model would be useful to study the efficiency of therapeutic treatments that could take place at the end of the grow-out period. Salmonella-free chicks were obtained from a breeder flock vaccinated with Salmonella. Intestinal maternal immunity was monitored by ELISA analyses at 2, 9, and 16 d of age. Data indicated that protection of maternal origin was not maintained over time and was drastically reduced at 9 d of age (P < 0.01). At 21 d of age, chickens were orally inoculated with Salmonella Typhimurium. The effects of the oral challenge dose (0, 3 × 10(3), 3 × 10(6), and 3 × 10(9) cfu/bird) and vancomycin pretreatment (no administration or 25 mg/bird) on intestinal immune responses, growth performance, and Salmonella colonization of chickens were investigated. After infection, the mucosal immune response was rapid, with increased (P < 0.01) anti-Salmonella Typhimurium IgA titers measured at 8 d postinfection in intestinal contents. A linear relationship (P < 0.05) existed between specific IgA levels in intestinal and cecal contents and the challenge dose inoculated. None of the challenge protocols caused mortality or clinical symptoms after infection. Nevertheless, the experimental infection induced a significant deterioration of growth performance. The pretreatment with 25 mg of vancomycin at 3 h before Salmonella inoculation was able to establish stable infection rates among the population of 3-wk-old infected chickens. Nevertheless, Salmonella shedding was not stable over the rearing period, and the bacteria seemed to be naturally eliminated from most birds at 22 d postinfection. This natural clearance of the gut, which was related, at least in part, to the intestinal immune response, should limit the usability of the created mature challenge model within 1 to 2 wk after inoculation.

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“…Different lowercase letters within one organ indicate significant differences between treatment groups (one-way ANOVA with subsequent Tukey's comparison of means, P Ͻ 0.05). tinal enzymes (27,28). Thereby the volume of the yolk sac decreases with time.…”

Section: Discussionmentioning

confidence: 99%

Antiviral Activity of Lambda Interferon in Chickens

Reuter

Soubies

Härtle

et al. 2014

J Virol

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A role played by the vitelline diverticulum in the yolk sac resorption in young post-hatched chickens

Cited by 45 publications

References 10 publications

The chick embryo yolk sac membrane expresses nutrient transporter and digestive enzyme genes

The chick embryo yolk sac membrane expresses nutrient transporter and digestive enzyme genes

Salmonella Typhimurium oral challenge model in mature broilers: Bacteriological, immunological, and growth performance aspects

Antiviral Activity of Lambda Interferon in Chickens

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