A role for alternative pathway catecholamines in the regulation of steroidogenesis in cow luteal cells

Abstract

Summary. Incubation of bovine luteal cells with the alternative pathway catecholamines octopamine, synephrine and deoxyadrenaline at concentrations of 10 \ m=-\ 6 to 10\m=-\3 m enhanced the production of progesterone (P < 0\m=.\05).Tryamine did not alter basal progesterone production (P > 0\m=.\05). Addition of noradrenaline and adrenaline at concentrations of 10 \ m=-\ 4 to 10\m=-\7 m significantly elevated the production of progesterone (P < 0\m=.\05). The steroidogenic response to noradrenaline and adrenaline was greater than that for octopamine, synephrine and deoxyadrenaline (P < 0\m=.\05).Response to both primary (10\m=-\6 m) and alternative (10\m=-\4 m) pathway catecholamines was inhibited by propranolol (10\m=-\5m, P < 0\m=.\05) but not phentolamine (10\m=-\5m, P > 0\m=.\05). These results demonstrate that octopamine, synephrine and deoxyadrenaline can affect steroidogenesis by bovine luteal cells, and their action is mediated by \g=b\-adrenergicreceptors.

Luteotropic Mechanisms in the Bovine Corpus Luteum: Role of Oxytocin, Prostaglandin F₂ _&alpha;, Progesterone and Noradrenaline

“…Condon and Black [85] demonstrated that catecholamines stimulate P4 secretion in bovine CL in vitro. This observation was confirmed by many experiments both in vitro [20,29,56,82,86,87] and in vivo [31,32,88]. Catecholamines also stimulate the synthesis and release of OT [20,29,31].…”

“…Noradrenaline regulates the secretory function of bovine CL by activation of β-adrenergic receptors [31,[84][85][86][87][88][89][90][91]. On the other hand, NA can also increase the pressure of the blood supply to the bovine CL by stimulation of β-adrenergic receptors [78][79][80].…”

Biogenic amine regulation of bovine luteal progesterone production in vivo

“…In addition to the absence of direct adrenergic innervation, we have been unable to detect noradrenaline or adrenaline in bovine luteal tissue using HPLC analysis (Battista & Condon, 1986b), but serotonin was detected (Battista & Condon, 1986a). Possible sources of luteal serotonin include mast cells, blood platelets, storage of peripherally circulating serotonin or de-novo syn¬ thesis.…”

Regional differences in catecholamine concentrations in bovine ovaries analysed by high-performance liquid chromatography

“…Recently Battista et al (1989) reported similar levels in bovine corpora lutea on days 10-12 of the oestrous cycle (noradrenaline 10-2 + 2-5 ng/g; dopamine 41-9 + 5-7 ng/g). These data provide a physiological basis for the catecholamine stimulation of progesterone and oxytocin synthesis by bovine luteal and granulosa cells (Condon & Black, 1976;Battista & Condon, 1986;Luck & Jungclas, 1987) and for the presence of ß-receptors in luteal tis¬ sue (Abramovitz, Iyengar & Birnbaumer, 1982;Norjavaara, Rosberg, Gäfvels & Selstam, 1984;Perkins, Cronin & Veldhuis, 1986). Further support for the role of catecholamines in luteal function has been demonstrated in vivo by Weiss, Dail & Ratner (1982) who found that electrical stimulation of the superior ovarian nerve enhanced progesterone production in the rat, and also by Burden & Lawrence (1977) who reported that denervation or chemical sympathectomy decreased 3ß-hydroxysteroid dehydrogenase activity in corpora lutea of pregnant rats.…”