Biogenic amine regulation of bovine luteal progesterone production in vivo

Abstract

Biogenic amines were administered using osmotic pumps placed subcutaneously in the neck region of regularly cycling, non-lactating dairy cows on Days 9-11 (oestrus = Day 0) of the oestrous cycle. Blood samples were collected using indwelling jugular catheters and the plasma progesterone concentrations were measured. Samples were collected at 4-h intervals for the first 12 h of treatment and thereafter at 12-h intervals for the remainder of the 72-h treatment period. After administration of various doses of noradrenaline, adrenaline and serotonin (0.5-2.0 micrograms/kg/h) significant elevation of plasma progesterone was achieved at a dosage of 2.0 micrograms/kg/h (P less than 0.01). The response to adrenaline was greater than that observed for noradrenaline and serotonin (P less than 0.05). Within-treatment comparison to pretreatment samples showed plasma progesterone concentrations to increase within 4 h after the administration of noradrenaline, adrenaline and serotonin (P less than 0.05) and this enhancement was maintained throughout the treatment period (P less than 0.05). The elevation in plasma progesterone concentrations induced by noradrenaline, adrenaline and serotonin was independent of changes in circulating concentrations of luteinizing hormone. These results support a physiological role for endogenous biogenic amines in the control of bovine luteal progesterone production.

Beta-Adrenergic Receptors in Corpora Lutea from Different Stages of the Estrous Cycle in Conscious and Slaughtered Cattle1

“…This suggests that catecholamines can stimulate not only progesterone release but also its synthesis. The significant decrease in progesterone secretion after treatment of bovine CL with P-antagonists both in vitro [5] and in vivo [6,7] confirms this supposition.…”

“…L-[Propyl-2,3,-3 H]Dihydroalprenolol ( 3 H-DHA; specific activity 53.1 Ci/mmol) and [1,2,6, H]progesterone (specific activity 85.9 Ci/mmol) were purchased from Amersham (Bucks, UK); phentolamine from Ciba-Geigy (Basel, Switzerland); polocaine and aspirin from Polfa (Kutno, Poland); BSA (fraction V), HCl, sucrose, and ethanol from POCh (Gliwice, Poland); ()norepinephrine, ()epinephrine, TRISbase, (+)isoproterenol, alprenolol, L-ascorbic acid, progesterone, sodium octanesulfonate, adrenaline, dihydroxybenzylamine (DHBA), and noradrenaline from Sigma Chemical Co. (St. Louis, MO); Alumina A from Wohlem Pharma (Eschwege, Germany); Whatman filters from Whatman International Ltd. (Maidstone, UK); Na 2 HPO 4 , KH 2 PO 4 , citric acid, (EDTA)Na 2 , Na 2 S 2 0 5 , HCl, H 3 PO 4 , acetic acid, trichloroacetic acid (TCA), and methanol from Merck (Darmstadt, Germany); and xylazine and Oestrophan from Spofa (Prague, Czechoslovakia). Water for HPLC was prepared by deionization and sequential distillation.…”

“…Group (Gr) I ovaries were obtained in the autumn at a commercial slaughterhouse from sexually mature heifers or cows (n = 20) of the Black and White Lowland breed within 20-30 min after slaughter. CL were dissected free of the fibrous capsule, weighed, and classified into four (n = 5) subgroups corresponding to times during the estrous cycle according to the morphological criteria of color, consistency, size, and associated follicle maturation, i.e., [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. CL were then placed in liquid nitrogen.…”

“…Much evidence suggests that the sympathetic innervation of the ovary plays an important role in CL function of cattle [5,6,8]. The stimulatory influence of catecholamines on secretion of progesterone and ovarian oxytocin from CL of cattle has been found to be exerted through -receptors in both in vitro [5] and in vivo studies [7,8].…”

Local Release of Progesterone and Oxytocin from Microdialyzed Corpus Luteum in Superovulated Ewes: Characterization during the Non-breeding Season.

“…Such assumption is substantiated by the findings showing innervation of the normal bovine CL with adrenergic [25][26][27][28][29][30][31] as well as peptidergic nerves [32]. It would be possible that an episodic influence of innervation exist at any period of the experiment, but the high secretory activity of the CL up to Days 12-13 may mask such a basic function, and a direct influence may appear only after a decrease in the CL capacity to secrete P.…”