A robust method for testing urinary iodine using a microtitre robotic system

Mina, Ashraf; Favaloro, Emmanuel J.; Koutts, Jerry

doi:10.1016/j.jtemb.2011.09.001

Cited by 8 publications

(9 citation statements)

References 22 publications

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“…The Sandell-Kolthoff reaction was used for the determination of iodine using conventional batch [4,150,151] or flow injection [152] devices. Some automated [153,154] and robotic [155] systems are also used and microtitration devices have been used to reduce the amount of waste and to improve sample throughput [150,155,156]. The Sandell-Kolthoff reaction has been used for iodine determination in serum [157] and food [151] samples, but its main application is for urine analysis [150,153,154,156].…”

Section: Spectrophotometric Methodsmentioning

confidence: 99%

Analytical methods for the determination of halogens in bioanalytical sciences: a review

et al. 2013

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Fluorine, chlorine, bromine, and iodine have been studied in biological samples and other related matrices owing to the need to understand the biochemical effects in living organisms. In this review, the works published in last 20 years are covered, and the main topics related to sample preparation methods and analytical techniques commonly used for fluorine, chlorine, bromine, and iodine determination in biological samples, food, drugs, and plants used as food or with medical applications are discussed. The commonest sample preparation methods, as extraction and decomposition using combustion and pyrohydrolysis, are reviewed, as well as spectrometric and electroanalytical techniques, spectrophotometry, total reflection X-ray fluorescence, neutron activation analysis, and separation systems using chromatography and electrophoresis. On this aspect, the main analytical challenges and drawbacks are highlighted. A discussion related to the availability of certified reference materials for evaluation of accuracy is also included, as well as a discussion of the official methods used as references for the determination of halogens in the samples covered in this review.

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Section: Spectrophotometric Methodsmentioning

confidence: 99%

Analytical methods for the determination of halogens in bioanalytical sciences: a review

et al. 2013

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“…Hipertiroid merupakan suatu keadaan klinis yang ditimbulkan oleh sekresi berlebihan dari hormon tiroid yaitu tiroksin (T4) dan triiodotironin (T3). Akibatnya aktivitas tiroid menjadi tidak terkontrol, hal ini disebabkan oleh jumlah iodium yang berlebihan sehingga memblok fungsi tiroid dalam pembentukan hormon [4].…”

Section: Abner Tonu Lema Dkk: Pengembangan Metode Spektrofotometri Uunclassified

“…Untuk menggambarkan jumlah asupan iodium seseorang dapat didasarkan pada pengukuran kadar iodium di dalam urin (UEI/Urinary Excretion Iodine), karena sebagian besar iodium yang diabsorpsi dalam tubuh akhirnya akan diekskresi melalui urin [5]. Kandungan iodida dalam urin untuk penderita GAKI berat adalah <0,02 mg L -1 , untuk GAKI sedang 0,02-0,049 mg L -1 untuk GAKI ringan yaitu 0,05-0,099 mg L -1 [4]. Kadar iodida dalam urin normal adalah 0,1-0,199 mg L -1 [4], sedangkan kadar iodida dalam urin penderita hipertiroid (kelebihan iodida) adalah > 2,99 mg L -1 [6].…”

Section: Abner Tonu Lema Dkk: Pengembangan Metode Spektrofotometri Uunclassified

“…Kandungan iodida dalam urin untuk penderita GAKI berat adalah <0,02 mg L -1 , untuk GAKI sedang 0,02-0,049 mg L -1 untuk GAKI ringan yaitu 0,05-0,099 mg L -1 [4]. Kadar iodida dalam urin normal adalah 0,1-0,199 mg L -1 [4], sedangkan kadar iodida dalam urin penderita hipertiroid (kelebihan iodida) adalah > 2,99 mg L -1 [6]. Metode penentuan iodida yang sering digunakan adalah kromatografi dan spektrofotometri.…”

Section: Abner Tonu Lema Dkk: Pengembangan Metode Spektrofotometri Uunclassified

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Development of Spectrophotometric Methods for Iodide Determination Using Hydrogen Peroxide (H2O2) as Oxidizing

Lema

Sulistyarti

Atikah

2014

Nat-B

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ABSTRAKGangguan serius akibat kekurangan iodium dapat menyebabkan kretinisme, komplikasi kehamilan, gondok, terganggunya pembentukan hormon tiroid, keterbelakangan mental, penurunan fungsi kognitif. Untuk mendeteksi dini adanya Gangguan Akibat Kekurangan Iodium (GAKI), maka dikembangkan suatu metode spektrofotometri sederhana untuk penentuan iodida dengan menggunakan hidrogen peroksida (H2O2) sebagai oksidator. Penentuan iodida didasarkan pada reaksi oksidasi iodida (I -) menjadi iodium (I2) yang kemudian membentuk senyawa kompleks berwarna biru dengan amilum. Intensitas warna biru kompleks I2-amilum yang terbentuk diukur pada panjang gelombang maksimum yaitu 611 nm. Beberapa parameter yang telah dioptimasi yaitu: pembentukan kompleks dan waktu oksidasi iodida, konsentrasi optimum oksidator (H2O2), H2SO4, amilum dan pengaruh adanya ion pengganggu (klorida, bromida, tiosianat), dan diperoleh kondisi optimum: waktu pembentukan kompleks dan waktu oksidasi iodida selama 10 menit, H2O2 10 -1 M, H2SO4 5.10 -2 M, amilum 0,2 %. Pada kondisi optimum, metode menunjukkan linearitas 5-60 ppm, dengan limit deteksi 0,62 mg/L dan limit kuantisasi 2,09 mg/L. Metode ini telah berhasil diaplikasikan pada sampel sintetis dan sampel urin serta dapat direkomendasikan untuk mendeteksi kadar iodida pada penderita hipertiroid dan tablet KI.Kata kunci : iodida, amilum, hidrogen peroksida, spektrofotometri, urin ABSTRACT Serious disorders of iodine deficiency can cause irreversible cretinism, pregnancy complications, goiter, compromised thyroid hormone production, mental impairment and decreasing cognitive function. In order to detect early indication of Iodine Deficiency Disorders (IDD) a development of simple spectrophotometric method for iodide determination is proposed using hydrogen peroxide (H2O2) as an oxidizing agent. Determination of iodide is based on the oxidation reaction of I -to I2 that form blue complex compound I2-starch. The blue color intensity of the complex I2-starch produced was measured at wavelength of 611 nm. Several parameters were optimized: complex formation and oxidation time of iodide, the optimum concentration of oxidizing agent, H2SO4, starch and effect of interfering ions (chloride, bromide, thiocyanate), with optimum conditions obtained of respectively: 10 minute, H2O2 0.1 M, H2SO4 0.05 M, starch 0.2 %. Under these condition, the method showed linearity of 5 -60 ppm, limit of detection and quantification of 0.62 and 2.09 mg/L, respectively. This method has been successfully applied to synthetic and urine samples and can be recommended for the detection of iodide in patients hyperthyroidism and KI tablets.Keywords : iodide, starch, hydrogen peroxide, spectrophotometry, urine PENDAHULUANGangguan akibat kekurangan iodium (GAKI) merupakan masalah kesehatan masyarakat Indonesia yang perlu mendapatkan perhatian besar mengingat dampak negatif yang

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“…Such methods include electrochemical detection, spectrophotometry, chemiluminescence, polarography, mass chromatography, gas chromatography, liquid chromatography and ion chromatography [12 -19] . All these methods have some suboptimal properties [15,16] . For example, analytical methods, particularly inductivity coupled plasma mass spectroscopy, which is often considered the gold standard [14] , is time consuming and requires expensive and complex instrumentation [17] .…”

Section: Introductionmentioning

confidence: 99%

Potentiometric measurement of urinary iodine concentration in patients with thyroid diseases with and without previous exposure to non-radioactive iodine

Giovacchini¹,

Giovanella²,

Haldemann³

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

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A robust method for testing urinary iodine using a microtitre robotic system

Cited by 8 publications

References 22 publications

Analytical methods for the determination of halogens in bioanalytical sciences: a review

Analytical methods for the determination of halogens in bioanalytical sciences: a review

Development of Spectrophotometric Methods for Iodide Determination Using Hydrogen Peroxide (H2O2) as Oxidizing

Potentiometric measurement of urinary iodine concentration in patients with thyroid diseases with and without previous exposure to non-radioactive iodine

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