A rice serine carboxypeptidase-like gene OsBISCPL1 is involved in regulation of defense responses against biotic and oxidative stress

Liu, Huizhi; Wang, Xiaoe; Zhang, Huijuan; Yang, Ya; Ge, Xuming; Song, Fengming

doi:10.1016/j.gene.2008.05.006

Cited by 86 publications

(58 citation statements)

References 43 publications

(62 reference statements)

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“…To this respect, plant proteases are known to be involved both in plant development (Schaller, 2004) and defence (Liu et al, 2008). From previous work we have evidence of induction of plant defences, at both morphological (papillae and vacuoles) and histochemical levels in roots colonized by P. chlamydosporia (Bordallo et al, 2002).…”

Section: Discussionmentioning

confidence: 89%

Expression of serine proteases in egg-parasitic nematophagous fungi during barley root colonization

López-Llorca

Gómez-Vidal

Monfort

et al. 2010

Fungal Genetics and Biology

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a b s t r a c tNematophagous fungi Pochonia chlamydosporia and P. rubescens colonize endophytically barley roots. During nematode infection, serine proteases are secreted. We have investigated whether such proteases are also produced during root colonization. Polyclonal antibodies against serine protease P32 of P. rubescens cross-reacted with a related protease (VCP1) of P. chlamydosporia, but not with barley proteases. These antibodies also detected an unknown ca. 65-kDa protein, labeled hyphae and appressoria of P. chlamydosporia and strongly reduced proteolytic activity of extracts from fungus-colonized roots. Mass spectrometry (MS) of 32-kDa protein bands detected peptides homologous to VCP1 only in Pochonia-colonized roots. Peptides homologous to barley serine carboxypeptidases were found in 65 kDa bands of all roots. RT-PCR detected expression of VCP1 and a new P. chlamydosporia serine carboxypeptidase (SCP1) genes only in fungus-colonized roots. SCP1 shared limited sequence homology with VCP1 and P32. Expression in roots of proteases from nematophagous fungi could be greatly relevant for nematode biocontrol.

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Section: Discussionmentioning

confidence: 89%

Expression of serine proteases in egg-parasitic nematophagous fungi during barley root colonization

López-Llorca

Gómez-Vidal

Monfort

et al. 2010

Fungal Genetics and Biology

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“…The second wound-related gene (SvWound3) encodes for a putative serine carboxypeptidase. Serine carboxypeptidases belong to a large family of hydrolyzing enzymes that play several roles in cellular processes, including defence responses against pathogen infection and oxidative stress (Liu et al 2008). ), and they were thus proposed to be part of a defence mechanisms against the invading fungus.…”

Section: Discussionmentioning

confidence: 99%

Gene expression in mycorrhizal orchid protocorms suggests a friendly plant–fungus relationship

Perotto

Rodda

Benetti

et al. 2014

Planta

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Main conclusionOrchid mycorrhiza has been often interpreted as an antagonistic relationship. Our data on mycorrhizal protocorms do not support this view as plant defence genes were not induced, whereas some nodulinlike genes were significantly up-regulated.Orchids fully depend on symbiotic interactions with specific soil fungi for seed germination and early development. Germinated seeds give rise to a protocorm, a heterotrophic organ that acquires nutrients, including organic carbon, from the mycorrhizal partner. It has long been debated if this interaction is mutualistic or antagonistic. To investigate the molecular bases of the orchid response to mycorrhizal invasion, we developed a symbiotic in vitro system between Serapias vomeracea, a Mediterranean green meadow orchid, and the rhizoctonia-like fungus Tulasnella calospora. 454 pyrosequencing was used to generate an inventory of plant and fungal genes expressed in mycorrhizal protocorms, and plant genes could be reliably identified with a customized bioinformatic pipeline. A small panel of plant genes was selected and expression was assessed by real-time quantitative PCR in mycorrhizal and non-mycorrhizal protocorm tissues. Among these genes were some markers of mutualistic (e.g. nodulins) as well as antagonistic (e.g. pathogenesis-related and wound/stress-induced) genes. None of the pathogenesis or wound/stress-related genes were significantly up-regulated in mycorrhizal tissues, suggesting that fungal colonization does not trigger strong plant defence responses. In addition, the highest expression fold change in mycorrhizal tissues was found for a nodulin-like gene similar to the plastocyanin domaincontaining ENOD55. Another nodulin-like gene significantly more expressed in the symbiotic tissues of mycorrhizal protocorms was similar to a sugar transporter of the SWEET family. Two genes coding for mannose-binding lectins were significantly up-regulated in the presence of the mycorrhizal fungus, but their role in the symbiosis is unclear.

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“…After tin5 mutant infections lipoxygenases and serine-carboxypeptidases were specifically upregulated, and both types of enzymes have been associated with defense [28], [29]. OsBISCPL1, a serine-carboxypeptidase from rice, was up-regulated in incompatible interactions between rice and the blast fungus, and was implicated in regulation of defense responses from heterologous expression studies [30]. In S. reilianum three orthologs of tin4 are present ( sr10075 , sr10077 and sr10079 ; Figure 2).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Largest Effector Gene Cluster of Ustilago maydis

et al. 2014

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In the genome of the biotrophic plant pathogen Ustilago maydis, many of the genes coding for secreted protein effectors modulating virulence are arranged in gene clusters. The vast majority of these genes encode novel proteins whose expression is coupled to plant colonization. The largest of these gene clusters, cluster 19A, encodes 24 secreted effectors. Deletion of the entire cluster results in severe attenuation of virulence. Here we present the functional analysis of this genomic region. We show that a 19A deletion mutant behaves like an endophyte, i.e. is still able to colonize plants and complete the infection cycle. However, tumors, the most conspicuous symptoms of maize smut disease, are only rarely formed and fungal biomass in infected tissue is significantly reduced. The generation and analysis of strains carrying sub-deletions identified several genes significantly contributing to tumor formation after seedling infection. Another of the effectors could be linked specifically to anthocyanin induction in the infected tissue. As the individual contributions of these genes to tumor formation were small, we studied the response of maize plants to the whole cluster mutant as well as to several individual mutants by array analysis. This revealed distinct plant responses, demonstrating that the respective effectors have discrete plant targets. We propose that the analysis of plant responses to effector mutant strains that lack a strong virulence phenotype may be a general way to visualize differences in effector function.

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A rice serine carboxypeptidase-like gene OsBISCPL1 is involved in regulation of defense responses against biotic and oxidative stress

Cited by 86 publications

References 43 publications

Expression of serine proteases in egg-parasitic nematophagous fungi during barley root colonization

Expression of serine proteases in egg-parasitic nematophagous fungi during barley root colonization

Gene expression in mycorrhizal orchid protocorms suggests a friendly plant–fungus relationship

Characterization of the Largest Effector Gene Cluster of Ustilago maydis

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