A rice FRD3-like (<i>OsFRDL1</i>) gene is expressed in the cells involved in long-distance transport

Inoue, Haruhiko; Mizuno, Daichi; Takahashi, Michiko; Nakanishi, Hiromi; Mori, Satoshi; Nishizawa, Naoko K.

doi:10.1080/00380768.2004.10408586

Cited by 53 publications

(25 citation statements)

References 33 publications

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“…7, A and B). This result is consistent with OsFRDL1 promoter GUS staining (Inoue et al, 2004). No signal was observed in the knockout line (Fig.…”

Section: Expression Pattern Of Osfrdl1 and Localization Of Osfrdl1supporting

confidence: 81%

“…7C), indicating the high specificity of the anti-OsFRDL1 antibody. The subcellular localization of OsFRDL1 was found to be localized on the plasma membrane by using heterologous expression in the onion (Allium cepa) epidermal cells (data not shown), which was the same as that reported previously (Inoue et al, 2004).…”

Section: Expression Pattern Of Osfrdl1 and Localization Of Osfrdl1supporting

confidence: 78%

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OsFRDL1 Is a Citrate Transporter Required for Efficient Translocation of Iron in Rice

et al. 2008

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Multidrug and toxic compound extrusion (MATE) transporters represent a large family in plants, but their functions are poorly understood. Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1), the closest homolog of barley (Hordeum vulgare) HvAACT1 (aluminum [Al]-activated citrate transporter 1), in terms of metal stress (iron [Fe] deficiency and Al toxicity). This gene was mainly expressed in the roots and the expression level was not affected by either Fe deficiency or Al toxicity. Knockout of this gene resulted in leaf chlorosis, lower leaf Fe concentration, higher accumulation of zinc and manganese concentration in the leaves, and precipitation of Fe in the root's stele. The concentration of citrate and ferric iron in the xylem sap was lower in the knockout line compared to the wild-type rice. Heterologous expression of OsFRDL1 in Xenopus oocytes showed transport activity for citrate. Immunostaining showed that OsFRDL1 was localized at the pericycle cells of the roots. On the other hand, there was no difference in the Al-induced secretion of citrate from the roots between the knockout line and the wild-type rice. Taken together, our results indicate that OsFRDL1 is a citrate transporter localized at the pericycle cells, which is necessary for efficient translocation of Fe to the shoot as a Fe-citrate complex.

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“…7, A and B). This result is consistent with OsFRDL1 promoter GUS staining (Inoue et al, 2004). No signal was observed in the knockout line (Fig.…”

Section: Expression Pattern Of Osfrdl1 and Localization Of Osfrdl1supporting

confidence: 81%

Section: Expression Pattern Of Osfrdl1 and Localization Of Osfrdl1supporting

confidence: 78%

OsFRDL1 Is a Citrate Transporter Required for Efficient Translocation of Iron in Rice

et al. 2008

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“…Moreover, abnormal Fe has been detected in frd3 mutants, indicating that AtFRD3 is responsible for loading citrate, a chelator of Fe, into root xylem for efficient Fe translocation ( Figure 5) (Durrett et al 2007;Green and Rogers 2004). The rice ortholog of AtFRD3, OsFRDL1 (Oryza sativa frd-like 1) has a similar mechanism of action, indicating that this mechanism is widely conserved in angiosperm (Inoue et al 2004;Yokosho et al 2009). In contrast, Fe acquisition mechanisms from soil have been found to differ, with gramineous plants using mugineic acid to chelate Fe (strategy II) and other plants reducing ferric ion with ferric reductase (strategy I) to increase Fe solubility (Kobayashi and Nishizawa 2012;Römheld 1987).…”

Section: Fe Translocationmentioning

confidence: 94%

The multidrug and toxic compound extrusion (MATE) family in plants

Takanashi

Shitan

Yazaki

2014

Plant Biotechnology

124

113

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Multidrug and toxic compound extrusion (MATE) transporters are a family of cation antiporters occurring in most organisms from prokaryotes to eukaryotes. This family constitutes one of the largest transporter families in plants, with, for example, more than 50 MATE genes in the Arabidopsis genome. Moreover, MATE transporters are involved in a wide variety of physiological functions throughout plant development, transporting a broad range of substrates such as organic acids, plant hormones and secondary metabolites. This review categorizes plant MATE transporters according to their physiological roles and summarizes their tissue specificity, membrane localization, and transport substrates. We also review the molecular evolutionary development of plant MATE transporters.

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“…The characterization of FRD3 both identified a pathway for citrate excretion from the pericycle cells to the xylem and supported the importance of iron–citrate complexes for iron translocation from the roots to the shoots [78] (see note added in proof). The closest homologue of AtFRD3 in rice, OsFRDL1, is also involved in iron translocation from roots to shoots [79,122]. AtFRD3 and OsFRDL1 are expressed in root pericycle cells.…”

Section: Root Anion Channels: Anion Excretion and Loading Into The Xylemmentioning

confidence: 99%

Anion channels in plant cells

et al. 2011

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Plant anion channels allow the efflux of anions from cells. They are involved in turgor pressure control, changes in membrane potential, organic acid excretion, tolerance to salinity and inorganic anion nutrition. The recent molecular identification of anion channel genes in guard cells and in roots allows a better understanding of their function and of the mechanisms that control their activation.

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A rice FRD3-like (OsFRDL1) gene is expressed in the cells involved in long-distance transport

Cited by 53 publications

References 33 publications

OsFRDL1 Is a Citrate Transporter Required for Efficient Translocation of Iron in Rice

OsFRDL1 Is a Citrate Transporter Required for Efficient Translocation of Iron in Rice

The multidrug and toxic compound extrusion (MATE) family in plants

Anion channels in plant cells

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