A Rice Dual-localized Pentatricopeptide Repeat Protein is involved in Organellar RNA Editing together with OsMORFs

Xiao, Haijun; Xu, Yanghong; Ni, Chenzi; Zhang, Qiannan; Zhong, Feiya; Huang, Jishuai; Liu, Wei; Peng, Leilei; Zhu, Yong‐Guan; Hu, Jun

doi:10.1093/jxb/ery108

Cited by 29 publications

(47 citation statements)

References 54 publications

(64 reference statements)

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“…To test the binding site specificity of DUA1 more directly, we performed a REMSA. In a REMSA assay, protein binding to an RNA target results in a mobility shift that is detectable through gel electrophoresis (Xiao et al ., ,b). A recombinant DUA1 fusion protein (GST−DUA1 77‐448) was generated and the purified recombinant protein was used for REMSA.…”

Section: Resultsmentioning

confidence: 99%

“…We performed REMSA assay as described previously (Xiao et al ., ,b). The corresponding cDNA fragments of DUA1 were amplified with specific primers (DU‐4TF/T) and cloned into the pGEX‐4T‐1 vector to create the fusion protein glutathione‐ S ‐transferase (GST)−DUA1 (Clontech 639648).…”

Section: Methodsmentioning

confidence: 99%

“…Pentatricopeptide repeat (PPR) proteins form the largest family of proteins known to date that are directly involved in posttranscriptional modification in higher plants. Accumulating data indicate that PPR family members serve diverse functions in posttranscriptional modification, such as RNA editing (Ichinose & Sugita, ; Jiang et al ., ; Xiao et al ., ,b), RNA splicing (Ren et al ., ; Huang et al ., ; Tadini et al ., ), RNA processing (Rackham et al ., ; S. Wang et al ., ; Stoll et al ., ), RNA stability (Hammani et al ., ; Zoschke et al ., ; Y. Zhang et al ., ), RNA translation (Zhou et al ., ; Legen et al ., ), and RNA maturation (W. Wu et al ., ). The PPR gene family comprises 450 members in Arabidopsis and 477 members in rice (Saha et al ., ; Barkan & Small, ).…”

Section: Introductionmentioning

confidence: 99%

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The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice

Cui

Wang

et al. 2018

New Phytologist

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Summary Low temperature stress hinders plant growth and chloroplast development and can limit the geographic range of cultivars. In rice, japonica cultivars have greater chilling tolerance than indica cultivars, but the molecular mechanism underlying chilling tolerance is unclear. Here, we report an RNA‐binding protein, DUA1, cloned from the indica cultivar Dular, which exhibits a deficiency in chloroplast development at an early stage of development under low‐temperature conditions. DUA1 shares high sequence homology with the pentatricopeptide repeat family and functions in plastid RNA editing under low‐temperature conditions. Our data suggest that DUA1 can bind to the plastid‐encoded rps8‐182 transcript and disruption of DUA1 activity impairs editing. The RNA editing cofactor WSP1, a partner of DUA1, also participates in chloroplast development at low temperature. Western blot analysis indicates that WSP1 enhances DUA1 stability under low temperatures. DUA1 sequence analyses of rice core germplasm revealed that three major haplotypes of DUA1 and one haplotype showed substantial differences in chlorophyll content under low‐temperature conditions. Variation at DUA1 may play an important role in the adaptation of rice to different growing regions.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice

Cui

Wang

et al. 2018

New Phytologist

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“…PPR family proteins are present in most eukaryotes (Lurin et al 2004; Schmitz‐Linneweber and Small 2008), and this family is one of the largest protein families in plants, with most members localized to either the mitochondria or chloroplasts (Lurin et al 2004). Some PPR proteins are dual‐localized to the mitochondria and chloroplasts (Sosso et al 2012; Li et al 2018; Xiao et al 2018), and a few are targeted to the nucleus (Ding et al 2006), whereas SOAR1 is dual‐localized to the cytosol and nucleus (Jiang et al 2014). As we know, PNM1 is a PPR protein previously reported to localize to both the nucleus and mitochondria (Hammani et al 2011).…”

Section: Discussionmentioning

confidence: 99%

DEK43 is a P‐type pentatricopeptide repeat (PPR) protein responsible for the Cis‐splicing of nad4 in maize mitochondria

Ren

Wang

Zhang

et al. 2019

JIPB

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Mitochondria, the main energy transducers in plant cells, require the proper assembly of respiratory chain complexes I–V for their function. The NADH dehydrogenase 4 (nad4) gene encodes mitochondrial respiratory chain complex I subunit IV, but the mechanism underlying nad4 transcript splicing is unclear. Here, we report that the P‐type pentatricopeptide repeat (PPR) protein DEFECTIVE KERNEL 43 (DEK43) is responsible for cis‐splicing of the nad4 transcript in maize. We demonstrate that DEK43 localizes to both the nucleus and mitochondria. The mutation of Dek43 resulted in embryo‐lethal and light‐colored defective kernels. Among the 22 mitochondrial group II introns, the splicing efficiency of nad4 introns 1 and 3 was reduced by up to 50% compared to the wild type. The levels of complex I and supercomplex I+III2 were also reduced in dek43. Furthermore, in‐gel NADH dehydrogenase assays indicated that the activities of these complexes were significantly reduced in dek43. Further, the mitochondrial ultrastructure was altered in the mutant. Together, our findings indicate that DEK43, a dual‐localized PPR protein, plays an important role in maintaining mitochondrial function and maize kernel development.

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“…In rice, a total of 477 PPR proteins are found, among which several PLS‐type PPR proteins, MPR25, OGR1, OsSMK1, OsEMP5 and OsPGL1, have been reported to be involved in mitochondrial RNA editing (Kim et al ., ; Toda et al ., ; Liu et al ., ; Li et al ., ; Xiao et al ., ). By contrast, mitochondrion‐targeted P‐type PPR proteins in rice were identified as restorers of cytoplasmic male sterility which function in preventing translation of sterility‐inducing open reading frames (ORFs; Wang et al ., ; Hu et al ., ; Luo et al ., ; Huang et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Rice FLOURY ENDOSPERM10 encodes a pentatricopeptide repeat protein that is essential for the trans‐splicing of mitochondrial nad1 intron 1 and endosperm development

Ren

Cai

et al. 2019

New Phytologist

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Summary Endosperm, the major storage organ in cereal grains, determines grain yield and quality. Despite the fact that a role for P‐type pentatricopeptide repeat (PPR) proteins in the regulation of endosperm development has emerged, molecular functions of many P‐type PPR proteins remain obscure. Here, we report a rice endosperm defective mutant, floury endosperm10 (flo10), which developed smaller starch grains in starchy endosperm and abnormal cells in the aleurone layer. Map‐based cloning and rescued experiments showed that FLO10 encodes a P‐type PPR protein with 26 PPR motifs, which is localized to mitochondria. Loss of function of FLO10 affected the trans‐splicing of the mitochondrial nad1 intron 1, which was accompanied by the increased accumulation of the nad1 exon 1 and exons 2–5 precursors. The failed formation of mature nad1 led to a dramatically decreased assembly and activity of complex I, reduced ATP production, and changed mitochondrial morphology. In addition, loss of function of FLO10 significantly induced an alternative respiratory pathway involving alternative oxidase. These results reveal that FLO10 plays an important role in the maintenance of mitochondrial function and endosperm development through its effect on the trans‐splicing of the mitochondrial nad1 intron 1 in rice.

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A Rice Dual-localized Pentatricopeptide Repeat Protein is involved in Organellar RNA Editing together with OsMORFs

Abstract: A dual-localized PPR protein, OsPGL1, is required for simultaneous RNA editing in mitochondria and chloroplasts. OsPGL1 binds to two distinct target transcripts directly and cooperates with OsMORFs.

Cited by 29 publications

References 54 publications

The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice

The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice

DEK43 is a P‐type pentatricopeptide repeat (PPR) protein responsible for the Cis‐splicing of nad4 in maize mitochondria

Rice FLOURY ENDOSPERM10 encodes a pentatricopeptide repeat protein that is essential for the trans‐splicing of mitochondrial nad1 intron 1 and endosperm development

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