A review on current conventional and biotechnical approaches to enhance biosynthesis of steviol glycosides in Stevia rebaudiana

Basharat, Samra; Huang, Ziyang; Gong, Mengyue; Ahmed, Aqsa; Hussain, Muhammad Iftikhar; Li, Jianghua; Du, Guocheng; Liu, Long

doi:10.1016/j.cjche.2020.10.018

Cited by 29 publications

(13 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…SGs consist of a diterpenoid steviol backbone bonded with one to three glucosyl groups at the steviol C13-hydroxyl and/or C19-carboxylic acid positions (Figure ). The glycones at the R 1 and R 2 positions and the total number of β-glycosyl moieties in the molecule are related to the sweetness and taste of SGs. , However, as the main constitutes in the commercial SG sweeteners, the bitter aftertaste and astringency of rebaudioside A (RA) and stevioside (St) are an obstacle to wide acceptance and applications . Rebaudioside D (RD) and rebaudioside M (RM), among the minor SGs, possessing five or six glucose units, have high maximum sweet intensities and show the most reduced lingering bitter taste compared with St and RA. , It seems that extending the C-19-ester-linked glycosyl unit and increasing the total number of β-glycosyl moieties lead to improved sweetness and taste of SGs. , …”

Section: Introductionmentioning

confidence: 99%

“…The glycones at the R 1 and R 2 positions and the total number of β-glycosyl moieties in the molecule are related to the sweetness and taste of SGs. 1,7 However, as the main constitutes in the commercial SG sweeteners, the bitter aftertaste and astringency of rebaudioside A (RA) and stevioside (St) are an obstacle to wide acceptance and applications. 8 Rebaudioside D (RD) and rebaudioside M (RM), among the minor SGs, possessing five or six glucose units, have high maximum sweet intensities and show the most reduced lingering bitter taste compared with St and RA.…”

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Screening UDP-Glycosyltransferases for Effectively Transforming Stevia Glycosides: Enzymatic Synthesis of Glucosylated Derivatives of Rubusoside

Pan

Xiao

Tang

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Five plant-derived uridine diphosphate glycosyltransferases (UGTs) that catalyzed the glucosylation of stevia glycosides (SGs) were uncovered as the result of sequence mining considering the catalytic residues and conserved motifs of the known UGTs. Thereinto, LbUGT from Lycium barbarum with high activity toward rubusoside has been enzymatically characterized. The recombinant LbUGT was demonstrated to catalyze the β-1,6-glucosylation at C19 of rubusoside, producing a monoglucosyl derivative 13-[(O-β-D-glucopyranosyl) oxy] ent-kaur-16-en-19-oic acid-[(6-O-β-D-glucopyranosyl-β-D-glucopyranosyl) ester], which was then submitted to a β-1,2-glucosylation by LbUGT, resulting in a diglucosyl derivative 13-[(O-β-D-glucopyranosyl) oxy] entkaur-16-en-19-oic acid-[(2-O-β-D-glucopyranosyl-6-O-β-D-glucopyranosyl-β-D-glucopyranosyl) ester].The di-glycosylated product of rubusoside showed an obvious increase in sweetness intensity (134 times sweeter than 5% sucrose) and almost eliminated the unpleasant bitter taste. This work will provide a reference for the taste improvement of SGs.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Screening UDP-Glycosyltransferases for Effectively Transforming Stevia Glycosides: Enzymatic Synthesis of Glucosylated Derivatives of Rubusoside

Pan

Xiao

Tang

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Bu sebeple, gıda maddelerinde en çok tercih edilen tatlandırıcılardan biri olacağı varsayılmaktadır [9]. Stevia bitkisi steviol glikozitlerin yanı sıra flavonoid, fenolik bileşik ve alkoloid içermesinden dolayı tıbbi açıdan da büyük bir önem taşımaktadır [10,11]. Stevia bitkisinin biyoaktif bileşenlerinin kan basıncını ve kan şekerini düşürücü [8], bağışıklığı iyileştirici [12], kardiyovasküler ve renal sistemleri indükleyici [13] ve antitümör, antikaryojenik, antiinflamatuar [6] etkilerinin olduğu belirlenmiştir.…”

Section: Introductionunclassified

Physiological and Biochemical Effects of Drought Stress in Stevia (Stevia rebaudiana Bertoni)

YALÇIN

Torun

Eroğlu

et al. 2022

Düzce Üniversitesi Bilim Ve Teknoloji Dergisi

View full text Add to dashboard Cite

Drought has been limiting the productivity of agricultural crops by increasing alarmingly in recent years. This situation has increased the importance of research on the determination of plants resistant to arid conditions. In this study, two cultivars (Yalova and STF-4) of Stevia rebaudiana Bertoni plant were investigated physiological and biochemical responses of under drought stress. Plants were grown in controlled greenhouse conditions for 3 months and then exposed to drought for 3 weeks. The plants harvested after three weeks were measured leaf length, osmotic potential, relative water content (RWC), chlorophyll fluorescence (Fv/Fm), proline amount, hydrogen peroxide (H2O2) amount and lipid peroxidation level. In both cultivars of S. rebaudiana plant, when compared to the plants in the control group under drought stress, while a decrease in leaf length was detected, the highest decrease was found in Yalova cultivar (25.7%). However, both cultivars maintained their water status under drought stress. While Fv/Fm value was not affected by drought in STF-4 cultivar, Yalova cultivar decreased compared to the control. In the amount of proline was a difference between the cultivars. While STF-4 cultivar under arid conditions no change was observed in the amount of proline, increase of 42.9% occurred in Yalova cultivar. On the other hand, drought stress significantly increased the lipid peroxidation level in leaves. While this increase was 41.2% in Yalova cultivar, it was 21.1% in STF-4 variety. Differential response to drought stress between the two cultivars, was observed in H2O2 content. The amount of H2O2 under drought stress, while decreased by 42.7% in Yalova cultivar it increased by 5.5% in STF-4 variety. As a result, in the light of the measured parameters the STF-4 cultivar of S. rebaudiana plant was revealed that more drought tolerant.

show abstract

“…The main aim of this review is to encapsulate all the recent conventional and biotechnological advances made so far to enhance the SvGls production. Earlier studies have described the biosynthesis of SvGls and methods used to stimulate the production of SvGls in Stevia rebaudiana [7,13]. However, they fail to cover the current detailed information on the newly identified regulatory enzymes involved in the biosynthesis of SvGls.…”

Section: Introductionmentioning

confidence: 99%

Critical Review on Key Approaches to Enhance Synthesis and Production of Steviol Glycosides: A Blueprint for Zero-Calorie Sweetener

Rai

Han

2022

Applied Sciences

View full text Add to dashboard Cite

Steviol glycosides (SvGls) are plant secondary metabolites belonging to the class diterpenes. SvGls naturally derived from non-calorie sugar have therapeutic properties for diabetics, carcinogens, and anti-microbials. Over the past few years, SvGls have been extensively researched because of their extraordinary sweetness without side effects. SvGls are classified into several types based on the number of sugar bases attached to the steviol. Due to the difference in glycosylation, different SvGls have different sensory properties. One of the main obstacles is the mass production of SvGls. Novel physical and chemical treatments and advanced biotechnological approaches are introduced to increase the production of SvGls. Extraction of high-purity SvGls from plants is difficult, so researchers have manipulated the metabolic pathways of engineered microorganisms for bulk production of SvGls. There are many aspects related to the biochemistry and metabolism of SvGls, but their functional relationship with the S. rebaudiana is not well understood. Thus there is a need for in-depth research in this area. This review provides the readers with an overview of the research gaps and possible promising methodologies that can be utilized in the future. To trigger more research, this review encompasses the recent developments in SvGls production and marketing.

show abstract

A review on current conventional and biotechnical approaches to enhance biosynthesis of steviol glycosides in Stevia rebaudiana

Cited by 29 publications

References 92 publications

Screening UDP-Glycosyltransferases for Effectively Transforming Stevia Glycosides: Enzymatic Synthesis of Glucosylated Derivatives of Rubusoside

Screening UDP-Glycosyltransferases for Effectively Transforming Stevia Glycosides: Enzymatic Synthesis of Glucosylated Derivatives of Rubusoside

Physiological and Biochemical Effects of Drought Stress in Stevia (Stevia rebaudiana Bertoni)

Critical Review on Key Approaches to Enhance Synthesis and Production of Steviol Glycosides: A Blueprint for Zero-Calorie Sweetener

Contact Info

Product

Resources

About