A review of trends and limitations in hydrogel-rapid prototyping for tissue engineering

Billiet, Thomas; Vandenhaute, Mieke; Schelfhout, Jorg; Vlierberghe, Sandra Van; Dubruel, Peter

doi:10.1016/j.biomaterials.2012.04.050

Cited by 1,095 publications

(839 citation statements)

References 283 publications

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“…These drawbacks undermine the concept of employing hydrogels for 3D cell cultivation. 6,7 Several attempts have been proposed to solve this problem. [8][9][10][11][12] The Doyle group developed methods to generate hydrogel microparticles with specific shapes using continuous flow or stopflow lithography.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

et al. 2016

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We report a method to rapidly fabricate alginate hydrogel particles of specific sizes and shapes.Our method is based on the formation of arrays of droplets of pre-hydrogel solutions on superhydrophobic-hydrophilic patterns using the process of discontinuous dewetting, followed by their gelation via the parallel addition of CaCl 2 to the individual droplets via the sandwiching method. We demonstrate that viability of living cells incorporated within the hydrogel particles is higher during the long-term cultivation than in the case of cells cultured in the bulk threedimensional hydrogel matrix. Incorporation of magnetic particles into the free-standing hydrogel particles containing living cells enabled ease manipulation of the particles using an external magnetic field.

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Section: Introductionmentioning

confidence: 99%

Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

et al. 2016

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“…Hydrogels have a structure that is similar to the extracellular matrix (ECM) of many tissues, which will help with the cell integration [1,6,7]. Hydrogels also have the ability to homogenously encapsulate cells [1][2][3][4][5][6].…”

Section: Methodsmentioning

confidence: 99%

Study of Tissue Printing Parameters for Generating Complex Tissue Constructs

Navarro¹,

García²,

Sundaram³

et al. 2016

J Tissue Sci Eng

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A mixture of agarose, MEM IX and HeLa cells (dubbed Bio-Ink) was created to allow normal cell interaction with the scaffold material (agarose) before crosslinking as an initial step in 3D printing tissue. Bio-Ink was developed successfully as an in situ-scaffolding material for engineering biological structures. Bio-Ink has been further conditioned by adjusting agarose composition and gelling time to obtain optimal HeLa cell growth. After detailed study, the time range available for printing this material, before full crosslinking occurs, was determined to be about 300 s, giving it attractive properties for 3D printing. Repeatable 10 mm thick prints were successful, although more system calibration is still needed to achieve more complex prints.

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“…Most laser-based 3D printing systems are applicable for the hydrogel composite fabrications, which builds a 3D structures in a vat of photocurable hydrogels under the deposition of laser energy, usually UV range, in specific designed patterns [3] . The exposure of UV laser on to the surface of photocurable liquid causes gel-formation of a thin single layer, and it is sequentially moved upward or downward with the sample stage to allow the next layer formation on top of preformed structure.…”

Section: Laser-based Hydrogel 3d Printing Systemsmentioning

confidence: 99%

“…Since the advent of the first three-dimensional (3D) printing system, formerly known as additive manufacturing or rapid prototyping, in 1986, the manufacturing industry has undergone significant transformations, requiring now less time, energy, and producing less waste with the ability to directly fabricate 3D prototypes from computer-aided designs [1][2][3] . This fascinating ability to create 3D structures has already taken fabrication technology to a new level, especially in the field of tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

Jang¹,

Jung²,

Pan³

et al. 2018

ijb

182

117

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Three-dimensional (3D) printing of hydrogels is now an attractive area of research due to its capability to fabricate intricate, complex and highly customizable scaffold structures that can support cell adhesion and promote cell infiltration for tissue engineering. However, pure hydrogels alone lack the necessary mechanical stability and are too easily degraded to be used as printing ink. To overcome this problem, significant progress has been made in the 3D printing of hydrogel composites with improved mechanical performance and biofunctionality. Herein, we provide a brief overview of existing hydrogel composite 3D printing techniques including laser based-3D printing, nozzle based-3D printing, and inkjet printer based-3D printing systems. Based on the type of additives, we will discuss four main hydrogel composite systems in this review: polymer-or hydrogel-hydrogel composites, particle-reinforced hydrogel composites, fiber-reinforced hydrogel composites, and anisotropic filler-reinforced hydrogel composites. Additionally, several emerging potential applications of hydrogel composites in the field of tissue engineering and their accompanying challenges are discussed in parallel.

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A review of trends and limitations in hydrogel-rapid prototyping for tissue engineering

Cited by 1,095 publications

References 283 publications

Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

Study of Tissue Printing Parameters for Generating Complex Tissue Constructs

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

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