A review of toxoplasmosis in humans and animals in Turkey

Kolören, Zeynep; Dubey, J. P.

doi:10.1017/s0031182019001318

Cited by 30 publications

(17 citation statements)

References 95 publications

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“…As previously reported [ 9 ], young cats shed more oocysts (1–3 weeks) compared to adults. In the present study, two-thirds of the population of PCs at the Universiti Veterinary Hospital (UVH), Universiti Putra Malaysia (UPM), were adults based on weight and size [ 51 ] whereas, the ages of the FRCs were unknown. It is likely that the T. gondii oocysts detected in FRC faeces comes from young cats [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection and Genetic Diversity of Toxoplasma gondii Oocysts in Cat Faeces from Klang Valley, Malaysia, Using B1 and REP Genes in 2018

et al. 2020

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The major route for Toxoplasma gondii (T. gondii) infection is through the ingestion of foods contaminated with oocyst from cat faeces. The microscopic detection of T. gondii oocysts in cat faeces is challenging, which contributes to the failure of detecting or differentiating it from other related coccidian parasites. This study aims to detect T. gondii oocysts in cat faeces using two multicopy-target PCR assays and to evaluate their genetic diversity. Cat faecal (200) samples were collected from pet cats (PCs; 100) and free-roaming cats (FRCs; 100) within Klang Valley, Malaysia, and screened for coccidian oocysts by microscopy using Sheather’s sucrose floatation. PCR assays were performed on each faecal sample, targeting a B1 gene and a repetitive element (REP) gene to confirm T. gondii oocysts. Additionally, the PCR amplicons from the REP gene were sequenced to further confirm T. gondii-positive samples for phylogenetic analysis. Microscopy detected 7/200 (3.5%) T. gondii-like oocysts, while both the B1 gene and the REP gene detected 17/200 (8.5%) samples positive for T. gondii. All samples that were microscopically positive for T. gondii-like oocysts were also shown to be positive by both B1 and REP genes. The BLAST results sequenced for 16/200 (8.0%) PCR-positive T. gondii samples revealed homology and genetic heterogeneity with T. gondii strains in the GenBank, except for only one positive sample that did not show a result. There was almost perfect agreement (k = 0.145) between the two PCR assays targeting the B1 gene and the REP gene. This is the first report on microscopic, molecular detection and genetic diversity of T. gondii from cat faecal samples in Malaysia. In addition, the sensitivities of either the B1 gene or REP gene multicopy-target PCR assays are suitable for the accurate detection of T. gondii from cat faeces.

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Section: Discussionmentioning

confidence: 99%

Molecular Detection and Genetic Diversity of Toxoplasma gondii Oocysts in Cat Faeces from Klang Valley, Malaysia, Using B1 and REP Genes in 2018

et al. 2020

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“…Seropositivity for T. gondii infection in donkeys was reported as 30% in Northwestern Algeria [24] , 58.7% in Pakistan [26] , 47% in Iran [27] , 65.6% in Egypt [28] and 5%-8% in Italy [29] . While the seroprevalence in mules was found to be 23.8% in Brazil [30] and 15% in Spain [18] .…”

Section: Discussionmentioning

confidence: 90%

Seroprevalence of Toxoplasma gondii antibodies using Sabin- Feldman dye test among equines in Isparta province, Turkey

Aciöz¹,

Bozkaya

Babür³

2021

Parasitologists United Journal

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Background: Toxoplasmosis is a protozoan disease widely spread among different animal species with an incidence of up to 90%. The presence of this agent among equine species has been reported to be about 46.3%. T. gondii infection in equids could favor the spreading of the infection as equine carcasses are used for animal diets in zoos, as well as dead equine carcasses left outside villages are eaten by stray dogs and cats. This study was conducted to determine the seroprevalence of T. gondii among equine species (horses, donkeys, and mules) raised in Isparta province of Turkey by using Sabin Feldman dye test (SFDT) which is a reference test widely used for detecting T. gondii antibodies in different animal species. Objective: The aim of this study was to investigate the seroprevalence of T. gondii among equids in Isparta province, Turkey. Material and Methods: Blood samples were collected from 192 animals including horses (n=72), donkeys (n=88) and mules (n= 32) from January to December of 2016. Separated sera were tested for anti-T. gondii antibodies using SFDT. Results: Out of 192 samples, 44 (22.9%) reacted positive. Sero-positivity was 27.8%, 18.2% and 25.0% in horses, donkeys, and mules, respectively with no statistical difference. According to age of animals, the seroprevalence was 14.3%, 32.8%, and 20.8% in age groups 0-5, 6-10 and over 11 years, respectively with a statistically significant difference. Sero-positivity in male animals was 19.44% and in females 25.0%, with no statistical difference. Conclusion:The prevalence of exposure to T. gondii was determined as 22.9% in equids in Isparta province.

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“…In this way, infected rabbits can be a potential source of T. gondii for carnivores and humans (3). The previous studies have shown that T. gondii infection has a high seroprevalence rate in many species of warm-blooded animals including humans in many parts of the world (37,38), In Turkey, toxoplasmosis is widely prevalent in animals and the rate of seropositivity in humans is one third (39). In a recent study, Karakavuk et al (40) notified that the prevalence of T. gondii was determined to be molecularly 89.6% in wild birds.…”

Section: Encephalitozoon Cunculi Francisella Tularensis Toxoplasma Gondiimentioning

confidence: 99%

Seroprevalence of <i>Encephalitozoon cuniculi, Francisella tularensis</i> and <i>Toxoplasma gondii</i> in Zoonotic Diseases in European Hares (Lepus europaesus)

Özkan¹,

Yücesan²,

Babür³

et al. 2021

Turkiye Parazitol Derg

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Objective: European hares (Lepus europaeus) are among the most important animals that are connected with humans in many countries and natural life. Hares are important for public health, since they carry many zoonotic diseases, such as Encephalitozoon cuniculi, Francisella tularensis and Toxoplasma gondii. The study aimed to determine the seroprevalence of Encephalitozoon cuniculi, Francisella tularensis and T. gondii and the potential zoonotic risk posed by hares that live in provinces of Turkey.Methods: Blood samples were collected from hares during the official hunting season. Serum samples were examined serologically by enzyme-linked immunosorbent assay for E. cuniculi, Sabin-Feldman dye test was used to examine T. gondii, while micro-agglutination test was used to examine F. tularensis. Results:Of the total of 42 hares examined, one (2.4%) was found positive for E. cuniculi, two (4.8%) were found positive for T. gondii and one (2.4%) was found positive for F. tularensis. Conclusion:Anti-T. gondii and anti-E. cuniculi antibodies were serologically detected in hares for the first time in Turkey. Furthermore, this is the first study reporting the seropositivity of F. tularensis infection in hares.

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A review of toxoplasmosis in humans and animals in Turkey

Cited by 30 publications

References 95 publications

Molecular Detection and Genetic Diversity of Toxoplasma gondii Oocysts in Cat Faeces from Klang Valley, Malaysia, Using B1 and REP Genes in 2018

Molecular Detection and Genetic Diversity of Toxoplasma gondii Oocysts in Cat Faeces from Klang Valley, Malaysia, Using B1 and REP Genes in 2018

Seroprevalence of Toxoplasma gondii antibodies using Sabin- Feldman dye test among equines in Isparta province, Turkey

Seroprevalence of <i>Encephalitozoon cuniculi, Francisella tularensis</i> and <i>Toxoplasma gondii</i> in Zoonotic Diseases in European Hares (Lepus europaesus)

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