A review of tissue and organ viability assay

Malinin, Theodore I.; Perry, Vernon P.

doi:10.1016/s0011-2240(67)80140-8

Cited by 46 publications

(17 citation statements)

References 42 publications

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“…Each experiment was repeated a minimum of three times and data from a representative experiment are shown. A clonogenic assay (Malinin & Perry, 1967) Cell growth characteristics For the determination of doubling time, cell growth curves were established for each cell line by plotting cell number versus growth time. Doubling time was determined directly from the linear portion of the plot.…”

Section: Drugsmentioning

confidence: 99%

“…The growth characteristics of the sensitive, parental line (MCF7/S), DOX resistant variant (MCF7/D40) and Mitox resistant variant (MCF7/Mitox) are listed in Table I (Malinin & Perry, 1967) for Mitox with and without VER in all three cell lines (Table II). The IC50s from the clonogenic assays were generally lower than those obtained from the MTT assays.…”

Section: Growth Characteristicsmentioning

confidence: 99%

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Different mechanisms of decreased drug accumulation in doxorubicin and mitoxantrone resistant variants of the MCF7 human breast cancer cell line

Taylor

Dalton²,

Parrish³

et al. 1991

Br J Cancer

138

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Summary We selected two drug resistant variants of the MCF7 human breast cancer cell line by chronic in vitro exposure to doxorubicin (MCF7/D40 cell line) and mitoxantrone (MCF7/Mitox cell line), respectively. (Dalton, 1990). In such patients, clinical drug resistance (failure to respond to drugs which were initially effective) is a common phenomenon. Clinical drug resistance is likely due to a number of factors such as tumour growth kinetics, development of pharmacologic sanctuaries due to loss of vascular supply, development of hypoxia in large tumours, and development of structural and metabolic changes in individual tumour cells. Doxorubicin (DOX) is the most active single agent currently available for the treatment of breast cancer (Tormey, 1975). For previously treated and untreated patients the response to DOX as a single agent varies between 28 and 43% respectively. In addition, DOX is an integral component of many combination chemotherapy regimens for breast cancer.

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Section: Drugsmentioning

confidence: 99%

Section: Growth Characteristicsmentioning

confidence: 99%

Different mechanisms of decreased drug accumulation in doxorubicin and mitoxantrone resistant variants of the MCF7 human breast cancer cell line

Taylor

Dalton²,

Parrish³

et al. 1991

Br J Cancer

138

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“…Hoppe and Bavlster (1984) used the development of 8-cell hamster embryos into blastocysts or the expansion of bovine blastocysts, during a 24 hour period of culture, to assess the potential toxicity of the fluorescein diacetate assay. Although cellular alterations due to Insult may be observed immediately in some damaged cells, a 24 to 48 hour interval is frequently required for such changes to become evident (Evans 1963, Hallnin andPerry 1967). Furthermore, it has been recognized (Seidel 1981) that despite the Inadequacy of the present methods of bovine embryo culture, embryonic viability, as determined by pregnancy after transfer, does not begin to decline significantly unless the period of culture is extended beyond 24 hours.…”

Section: Effects Of Thermal Shock On Embryonic Development and The Stmentioning

confidence: 99%

“…Viability refers to the ability of an organism or some other form of "biological unit" to live and "implies the presence of a vital force in that unit" (Malinin and Perry 1967). Embryonic viability is frequently defined as the ability of the embryo to implant and develop into a live offspring.…”

Section: Assessment Of Preimplantation Embryonic Viabilitymentioning

confidence: 99%

“…However, it would be impossible, solely on the basis of ultrastructural cellular changes, to precisely define the time at which viability Is lost, because, as Cheville (1976) observed, cellular degeneration does not become cellular death until the point of irreversibility in the ongoing degenerative process is reached and that point, the exact moment of necrosis in the cell, is not precisely discernible. In addition, it is widely recognized in toxicity testing that both, the nature of the Insult and duration of exposure of the cell to an agent Influences the post-exposure response of the cell and that there may be a series of events which occur in dying cells (see Malinin and Perry, 1967, for review). In some cases, e.g.…”

Section: Effects Of Thermal Shock On Embryonic Development and The Stmentioning

confidence: 99%

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The use of eosin B to assess the viability and developmental potential of rat embryos

Dooley

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Assessment of membrane permeability in primary cultures of neurons and glia in response to osmotic perturbation

Fischel's¹,

Medzihradsky²

1985

J of Neuroscience Research

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In primary cultures of neurons and astrocytes from rat brain, the cellular contents of K+ and Na+ were used to assess plasma membrane permeability in response to osmotic shock. In contrast to trypan blue exclusion, the measure of ionic content reflected both transient and permanent membrane damage, and was also applicable to aggregates of cells. In steady state, the neurons and glia exhibited a K+ to Na+ ratio of 3-5, and the mean cellular contents (mu Eq/mg protein) of K+ and Na+ were 0.72 and 0.17 for astrocytes and 0.78 and 0.23 for neurons. Both hypo- and hypertonicity resulted in marked efflux of cell K+, but elevation of cellular Na+ occurred only under severe hypertonic conditions. Relative to neurons, astrocytes displayed considerably higher resistance to osmotic shock. During subsequent isotonic incubation, these cells were able to completely recover from transient membrane damage caused by a 10-min exposure to fourfold hypertonicity. Permanent changes in glial permeability were obtained only after a 20-min hypertonic shock. In contrast, 5 min hypertonic treatment of neurons decreased the ratio of cellular K+ to Na+ from 4.5 to 1. This ratio was restored twofold by isotonic incubation, but decreased permanently to below 1 after 10 min of hypertonic shock. The results describe marked differences in the osmotic fragility of neurons and glia and demonstrate that the determination of cellular K+ and Na+ provides a sensitive and accurate indicator of membrane permeability in neural cells propagated as surface-growing cultures. The approach has wide-ranging applicability.

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A review of tissue and organ viability assay

Cited by 46 publications

References 42 publications

Different mechanisms of decreased drug accumulation in doxorubicin and mitoxantrone resistant variants of the MCF7 human breast cancer cell line

Different mechanisms of decreased drug accumulation in doxorubicin and mitoxantrone resistant variants of the MCF7 human breast cancer cell line

The use of eosin B to assess the viability and developmental potential of rat embryos

Assessment of membrane permeability in primary cultures of neurons and glia in response to osmotic perturbation

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