A review of research progress of heterotrophic nitrification and aerobic denitrification microorganisms (HNADMs)

Song, Tao; Zhang, Xiaolei; Li, Ji; Wu, Xinyu; Feng, Haixia; Dong, Wenyi

doi:10.1016/j.scitotenv.2021.149319

Cited by 171 publications

(49 citation statements)

References 86 publications

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“…In recent years, the rapid development of intensive farming has led to a dramatic increase in the organic load on the waterbody, resulting in the environmental degradation of the farmed waterbody and the destruction of the ecological balance, causing severe losses to the industry [1]. In aquaculture, only about 25% of feed protein put into the waterbody is absorbed and used by aquatic animals, with most feed protein lost back into the waterbody in the form of feces, bait residues and secretions [2,3], leading to the accumulation of nitrogenous organic matter in the waterbody.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, it is essential to select and screen heterotrophic nitrifying bacteria that degrade ammonia nitrogen efficiently, are easy to culture and have a short growth cycle. Traditional theory holds that the nitrification function is mainly exercised by a group of autotrophic microorganisms [4], but in practice, researchers have found many limitations of autotrophic nitrifying bacteria in the process of ammonia removal [5]. And with the discovery of heterotrophic nitrification [6], heterotrophic nitrifying bacteria have gradually been isolated and screened, such as Pseudomonas putida [7,8], Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Bacillus subtilis Ab03 for efficient ammonia nitrogen removal

Wang

et al. 2022

Syst Microbiol and Biomanuf

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An efficient ammonia nitrogen degrading bacterial strain was isolated from a fish and shrimp pond and identified as Bacillus subtilis (Ab03). Firstly, the strain was continuously domesticated in ammonium solution to improve its nitrogen removal capacity. The performance of the strain in terms of nitrogen removal efficiency under different culture conditions was then examined. Finally, the nitrogen removal process and related strain mechanisms were analyzed by nitrogen balance. The results showed the strain Ab03 could remove 91.67% of NH 4 + -N at 300 mg/L under the conditions of glucose as the single carbon source, C/N of 15, pH of 7.5, the temperature of 35 ℃ and dissolved oxygen of 7-8 mg/L. It was also found that under conditions where ammonia nitrogen was the only nitrogen source, strain Ab03 could also undergo aerobic denitrification for simultaneous conversion, with a final gas conversion rate of 74.81%.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Bacillus subtilis Ab03 for efficient ammonia nitrogen removal

Wang

et al. 2022

Syst Microbiol and Biomanuf

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“…In the 1990s, a classical coupled model of ammonium nitrification in HNAD bacteria was reported (Wehrfritz et al, 1993). Ammonium is oxidized to hydroxylamine in the periplasm by ammonia monooxygenase (AMO), followed by oxidation to nitrite by hydroxylamine oxidoreductase (HAO), and finally to nitrate in the cytoplasm (Song et al, 2021). In HNADs, nitrate is reduced via denitrification by a series of oxidoreductases under aerobic conditions, and stable isotopes and enzyme inhibitors are used for more-accurate detection of intermediate metabolites in the study of nitrogen conversion pathways in HNADs.…”

Section: Ammonium Biotransformation Pathway In Hnadsmentioning

confidence: 99%

“…The reduction of nitrate to gaseous nitrogen by HNADs is accomplished via four reductases under aerobic conditions in four processes, that is, NO 3 − -N→NO 2 − -N→NO→N 2 O→N 2 (Song et al, 2021). Nitrite reductase is also a bottleneck in the denitrification pathway of HNADs because it is as sensitive to oxygen as are traditional anaerobic denitrifying bacteria.…”

Section: Nitrate Nitrite and Nitrous Oxide Biotransformation Pathways...mentioning

confidence: 99%

Advances in Research Into and Applications of Heterotrophic Nitrifying and Aerobic Denitrifying Microorganisms

Song²,

Wang³

et al. 2022

Front. Environ. Sci.

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With the increasing use of animal and plant proteins, pollution due to nitrogen sources is attracting increasing attention. In particular, the amount of nitrogen-containing sewage discharged into the environment has increased significantly, causing eutrophication of water bodies and environmental degradation of water quality. Traditionally, nitrifying bacteria perform ammonia nitrification under aerobic conditions, while denitrifying bacteria perform nitrate/nitrite denitrification under anaerobic conditions. However, heterotrophic nitrifying and aerobic denitrifying microorganisms (HNADs) perform ammonia nitrification and nitrate/nitrite denitrification under the same aerobic conditions using an organic carbon source, which is a much simpler and more efficient process. In this review, the distribution and evolutionary relationships of novel HNADs strains are presented, and the influencing factors, metabolic pathways, key enzymes, and practical applications of HNADs are reviewed.

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“…Biological processes that remove nitrogen from wastewater generally involve the transformation of different species of nitrogen into gaseous nitrogen, which is then released into the atmosphere without major risks [ 1 , 2 ]. Biological wastewater treatment plants are based on two processes: nitrification and denitrification.…”

Section: Introductionmentioning

confidence: 99%

The Potential Application of Natural Clinoptilolite-Rich Zeolite as Support for Bacterial Community Formation for Wastewater Treatment

et al. 2022

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The aim of this study was to investigate the use of natural zeolite as support for microbial community formation during wastewater treatment. Scanning electron microscopy (SEM), thermal decomposition and differential thermogravimetric curves (TGA/DGT) techniques were used for the physicochemical and structural characterization of zeolites. The chemical characterization of wastewater was performed before and after treatment, after 30 days of using stationary zeolite as support. The chemical composition of wastewater was evaluated in terms of the products of nitrification/denitrification processes. The greatest ammonium (NH4+) adsorption was obtained for wastewater contaminated with different concentrations of ammonium, nitrate and nitrite. The wastewater quality index (WWQI) was determined to assess the effluent quality and the efficiency of the treatment plant used, showing a maximum of 71% quality improvement, thus suggesting that the treated wastewater could be discharged into aquatic environments. After 30 days, NH4+ demonstrated a high removal efficiency (higher than 98%), while NO3+ and NO2+ had a removal efficiency of 70% and 54%, respectively. The removal efficiency for metals was observed as follows (%): Mn > Cd > Cr > Zn > Fe > Ni > Co > Cu > Ba > Pb > Sr. Analysis of the microbial diversity in the zeolite samples indicated that the bacteria are formed due to the existence of nutrients in wastewater which favor their formation. In addition, the zeolite was characterized by SEM and the results indicated that the zeolite acts as an adsorbent for the pollutants and, moreover, as a support material for microbial community formation under optimal conditions. Comparing the two studied zeolites, NZ1 (particle size 1–3 mm) was found to be more suitable for wastewater treatment. Overall, the natural zeolite demonstrated high potential for pollutant removal and biomass support for bacteria community growth in wastewater treatment.

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A review of research progress of heterotrophic nitrification and aerobic denitrification microorganisms (HNADMs)

Cited by 171 publications

References 86 publications

Characterization of Bacillus subtilis Ab03 for efficient ammonia nitrogen removal

Characterization of Bacillus subtilis Ab03 for efficient ammonia nitrogen removal

Advances in Research Into and Applications of Heterotrophic Nitrifying and Aerobic Denitrifying Microorganisms

The Potential Application of Natural Clinoptilolite-Rich Zeolite as Support for Bacterial Community Formation for Wastewater Treatment

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