Characterization of Bacillus subtilis Ab03 for efficient ammonia nitrogen removal

Wang, Qiang; Fu, Weilai; Lu, Ruiqi; Pan, Chunli; Yi, G. F.; Zhang, Xian; Rao, Zhiming

doi:10.1007/s43393-022-00088-6

Cited by 7 publications

(2 citation statements)

References 31 publications

(32 reference statements)

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“…Given those advantages with the high growth rate, B. subtilis NCIB3610 seemed to be the most promising host for aquaculture wastewater denitrification. The growth rate we observed for this strain on our AN (ammonium nitrate) medium - 0.34 h − 1 is much higher than the growth rate of the B. subtilis strain Ab03 as observed by Wang et al [ 18 ] and suggested for denitrification of polluted surface water.…”

Section: Discussioncontrasting

confidence: 61%

Exploring the potential of Bacillus subtilis as cell factory for food ingredients and special chemicals

Chen,

Brul,

Hugenholtz

2023

Microb Cell Fact

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Background Bacillus subtilis has been established as model microorganism for fundamental research in the laboratory on protein production/secretion and sporulation and as model bacterium for controlling spoilage in the food industry. It has also been used for production of (commercial) enzymes and several secondary metabolites such as vitamins. However, this doesn’t fully reflect the potential of B. subtilis as a cell-factory. Here, various strains of B. subtilis, including food-grade, spore-deficient strains and industrially used strains, were compared for their growth and metabolic potential. Industry-relevant parameters were analyzed for all strains under various aeration regimes, under anaerobic conditions, in various nutritious and nutrient-limited cultivation media, with and without organic nitrogen sources, and with and without sugar. Results Practical experiments were conducted to compare industrial relevant properties like growth rates, intracellular components and extracellular metabolite profile of different B. subtilis strains. Based on growth flexibility in different media, we found that some strains like NCIB3610 and DSM1092 are adapted to inorganic or organic nitrogen source utilization, which is highly relevant when considering a biorefinery approach using various cheap and abundant waste/sidestreams. Secondly, spore-deficient strains such as 3NA, 168 S and PY79S, showed advantages in microbial protein and acetolactate pathway expression, which is associated with applications in food industry for protein supplement and diacetyl production. Lastly, WB800 and PY79S exhibited potential for fermentative production of dipicolinic acid, 2,3-butanediol and lactic acid that could serve as precursors for biopolymers. Conclusion This study demonstrates the broad potential for more extensive industrial use of Bacillus subtilis in the (bio-based) chemical industry for use of sidestreams, in the personal care industry, in the food industry for food additive production, and in the bio-sustainable industry for biofuel and bio-degradable plastic precursors production. In addition, selecting different B. subtilis strains for specific purposes makes full use of the diversity of this species and increases the potential of B. subtilis in its contribution to the bio-based economy.

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Section: Discussioncontrasting

confidence: 61%

Exploring the potential of Bacillus subtilis as cell factory for food ingredients and special chemicals

Chen,

Brul,

Hugenholtz

2023

Microb Cell Fact

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“…and Ps. aeruginosa, have been previously reported in marine environments (Zhou et al, 2020;Wang et al, 2022). Bacillus species have been recognized for their significant roles in the nitrogen cycle, including nitrification (Zhao et al, 2020), denitrification (Li et al, 2020), and nitrogen fixation (Yusoff et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Oxidation of Ammonia in Fish Ponds to Nitrates Using Free and Immobilized Nitrifying Bacteria

Yusuf,

Rabiu Gamawa,

Haruna

2023

UJMR

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In fish farming, ammonia stands out as detrimental toxicant, with the potential to significantly impede growth and even lead to fish mortality. The adverse effects of ammonia exposure in aquatic environments primarily stem from elevated concentrations of unionized ammonium (NH3), which can readily permeate gill membranes. This study aimed at assessing the capability of free and mobilized forms of nitrifying bacteria in converting ammonia to nitrates. Nitrifying bacteria were isolated from water samples, resulting in the identification of two Pseudomonas species and two Bacillus species. The Bacillus sp., designated with the isolate code, B4 which exhibited the ability to convert ammonia to both nitrite and nitrate while demonstrating resilience to high ammonia concentrations. When Pseudomonas sp. (C4) and Bacillus sp. (B4) were utilized, the total ammonia concentration was reduced from 2 mg/L to 1.4 mg/L and 1.3 mg/L, respectively. Moreover, the immobilized forms of Pseudomonas sp. C4 and Bacillus sp. B4 achieved a more substantial reduction (75%), lowering the total ammonia concentration from 2 mg/L to 0.5 mg/L within 5-days period. Both nitrifying bacteria not only exhibited ammonia removal capabilities but also demonstrated their proficiency in transforming ammonia into nitrate. Immobilization proved effective in enhancing microbial tolerance to high ammonia concentrations, ultimately leading to improved water quality and the preservation of aquatic animal health.

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Exogenous compound bacteria enhance the nutrient removal efficiency of integrated bioremediation systems: Functional genes and microorganisms play key roles

Wei,

Shen,

Nicholaus

et al. 2024

Environmental Research

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Characterization of Bacillus subtilis Ab03 for efficient ammonia nitrogen removal

Cited by 7 publications

References 31 publications

Exploring the potential of Bacillus subtilis as cell factory for food ingredients and special chemicals

Exploring the potential of Bacillus subtilis as cell factory for food ingredients and special chemicals

Oxidation of Ammonia in Fish Ponds to Nitrates Using Free and Immobilized Nitrifying Bacteria

Exogenous compound bacteria enhance the nutrient removal efficiency of integrated bioremediation systems: Functional genes and microorganisms play key roles

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