A review of imaging techniques for systems biology

Kherlopian, Armen R.; Song, Ting; Duan, Qi; Neimark, Matthew A.; Po, Ming Jack; Gohagan, John K.; Laine, Andrew F.

doi:10.1186/1752-0509-2-74

Cited by 249 publications

(157 citation statements)

References 98 publications

(141 reference statements)

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“…The development of comprehensive models requires experimental information about the spatial and temporal arrangements of the network components as well as their structure, a challenge that required a multi--resolution approach and the combination of different techniques (Aloy and Russell, 2006;Kherlopian et al, 2008). Cryo--electron tomography (cryo--ET) is the ultimate technique to reveal the spatial organisation of protein structures and macromolecular complexes in single cells (Ben--Harush et al, 2010;Lučić et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Connecting μ-fluidics to electron microscopy

Kemmerling

Ziegler

Schweighauser

et al. 2012

Journal of Structural Biology

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A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic writing table to micro-pattern the EM grids. The method allows heterogeneous samples of minute volumes to be processed at physiological pH for structure and mass analysis, and allows the preparation characteristics to be finely tuned.

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Section: Introductionmentioning

confidence: 99%

Connecting μ-fluidics to electron microscopy

Kemmerling

Ziegler

Schweighauser

et al. 2012

Journal of Structural Biology

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“…Nanoshells are currently being investigated for micrometasis of tumours and also for treatment of diabetes mellitus. 11,12 Gold nanoshells can also detect immunoglobulins at a concentration range of nanograms per milliliter in plasma and whole blood. 11 Figure 3: Nanoshell 2.6 Nanoprobes Magnetic nanoprobes are used for cancer therapy.…”

Section: Nanoporesmentioning

confidence: 99%

Novel role of nanotechnology in medicine

Singla¹,

Singla²

2014

Int Jour of Biomed Res

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“…Imaging systems for the visualization of vascular networks have been reviewed extensively, e.g. in the context of blood flow modelling by Kim et al [46], angiogenesis by Kiessling et al [47] and systems biology by Kherlopian et al [48]. Here, we have created a scatter graph that compares maximum spatial resolution and tissue penetration depth (figure 4).…”

Section: Imaging Systems For Vascular Networkmentioning

confidence: 99%

Image-based modelling of skeletal muscle oxygenation

Zeller‐Plumhoff

Roose

Clough

et al. 2017

J. R. Soc. Interface.

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The supply of oxygen in sufficient quantity is vital for the correct functioning of all organs in the human body, in particular for skeletal muscle during exercise. Disease is often associated with both an inhibition of the microvascular supply capability and is thought to relate to changes in the structure of blood vessel networks. Different methods exist to investigate the influence of the microvascular structure on tissue oxygenation, varying over a range of application areas, i.e. biological in vivo and in vitro experiments, imaging and mathematical modelling. Ideally, all of these methods should be combined within the same framework in order to fully understand the processes involved. This review discusses the mathematical models of skeletal muscle oxygenation currently available that are based upon images taken of the muscle microvasculature in vivo and ex vivo. Imaging systems suitable for capturing the blood vessel networks are discussed and respective contrasting methods presented. The review further informs the association between anatomical characteristics in health and disease. With this review we give the reader a tool to understand and establish the workflow of developing an image-based model of skeletal muscle oxygenation. Finally, we give an outlook for improvements needed for measurements and imaging techniques to adequately investigate the microvascular capability for oxygen exchange.

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A review of imaging techniques for systems biology

Cited by 249 publications

References 98 publications

Connecting μ-fluidics to electron microscopy

Connecting μ-fluidics to electron microscopy

Novel role of nanotechnology in medicine

Image-based modelling of skeletal muscle oxygenation

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