A Reverse transcription-polymerase chain reaction (RT-PCR) based detection of foot-and-mouth disease in District Faisalabad, Pakistan during the Year 2016

Ali, Waqas; Habib, Md. Ahasan; Sajid, Sanaullah; Khan, Rashid Ahmed; Mazhar, Muhammad Usman; Khan, Irfan Ullah; Saliha, Uneeb; Farooq, Muhammad; Shah, Muhammad Salah Ud Din; Muzammil, Hafiz Muhammad

doi:10.26480/msm.01.2017.27.29

Cited by 13 publications

(13 citation statements)

References 18 publications

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“…The Polymerase chain reaction (PCR) technology has the potential to solve all these problems. For rapid diagnosis and sensitive detection, the PCR technique is ideal because this method has the potential to amplify target gene or DNA from a single copy [23,24].…”

Section: Introductionmentioning

confidence: 99%

Comparative Diagnosis of Typhoid Fever by Polymerase Chain Reaction and Widal Test in Southern Districts (Bannu, Lakki Marwat and D.I.KHAN) of Khyber Pakhtunkhwa, Pakistan

Khan¹,

Sajid²,

Javed³

et al. 2017

Acta sci. Malays

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Typhoid fever is a major dilemma in developing nations, despite the antibiotic use and new antibiotics discovery. Blood culture and serological tests (especially Widal test) that are regularly performed in Pakistan for diagnosis provide objectionable levels of false negative and false positive results, respectively. The present study was carried out at Kohat University of science and technology, Kohat, Pakistan from January 2013 to December 2013. A polymerase chain reaction (PCR) assay was compared with Widal test and blood culture for blood samples of 96 patients. In this study, blood from 25 healthy individuals was collected as a negative control. The detection rate of PCR was maximum (64.5%) followed by Widal test (26%) and blood culture (14.5%). Among the 82 (85.4%) samples that were found negative with blood culture, 48 (58.5%) were successfully detected by PCR and 11 (13.4%) were positive for the Widal test. Findings of this study showed that PCR is a fast and reliable technique for diagnosis of typhoid fever in suspected cases, as compared to frequently used conventional techniques like blood culture and Widal test.

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Section: Introductionmentioning

confidence: 99%

Comparative Diagnosis of Typhoid Fever by Polymerase Chain Reaction and Widal Test in Southern Districts (Bannu, Lakki Marwat and D.I.KHAN) of Khyber Pakhtunkhwa, Pakistan

Khan¹,

Sajid²,

Javed³

et al. 2017

Acta sci. Malays

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“…The cDNA synthesized during the previous step was utilized in a general, typing and sequencing PCR reaction assay. For general and typing PCR assay protocols optimized previously at our lab were followed [28,29] .…”

Section: Reverse Transcriptase Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Grup VII (Sind-08) Serotip Asya-1 Şap Hastalığı Virüsünün P1 Kodlayan Bölge Çeşitliliği

Ali¹,

Habib²,

Khan³

et al. 2018

Kafkas Univ Vet Fak Derg

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In this study, the capsid protein coding region of serotype Asia-1 viruses (n=131) were analyzed, giving importance to the viruses circulating since 2011 within the Group VII (Sind-08). The isolates recovered during 2011-2017 were found to group within the re-emerging cluster of Group VII (Sind-08). The time of the most recent common ancestor for this cluster was estimated to be approximately 2004. In comparison to the older isolates of Group VII (2001-2004), the re-emerging viruses showed variation at fourteen amino acid positions, including substitutions at the antigenically critical residues VP1140, VP1142 and VP277. In Group VII (Sind-08), all three major antigenic sites have mutations (Site I and II had four consensus changes at positions 140, 141 and 77, 79 respectively, while site IV had a replacement at position 59) relative to the internationally recommended vaccine strain (Shamir 89). This study also explains the development and optimization of a new RT-PCR method that may be employed to amplify and sequence a 2901 base pair (bp) section covering entire capsid coding region (P1) of the FMDV genome. This method offers a tool that can be employed for antigenic profiling and phylogenetic analyses of FMDV to help vaccine matching or strain selection in the episode of outbreaks.

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“…An important feature of the Pterocarpus pedatus Pierre is different from other Pterocarpus woods: the surface texture of the wood is extremely beautiful, like a bird's claws. In the Dalbergia odorifera, Pterocarpus pedatus Pierre fluorescence phenomenon is the most significant (Abbas et al, 2017, Ali et al, 2017). The application value of Pterocarpus pedatus Pierre lies mainly in heartwood.…”

Section: Introductionmentioning

confidence: 99%

GC–MS explores the health care components in the extract of Pterocarpus pedatus Pierre

Chen

2018

Saudi Journal of Biological Sciences

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Pterocarpus a high-end, expensive furniture materials collectively. Pterocarpus and Pterocarpus products have a certain human health function. Therefore, this paper to Pterocarpus pedatus Pierre as an example, to study its extract on human health beneficial health care ingredients. FT-IR analysis showed that the infrared transmittance of Pterocarpus pedatus Pierre powder after ethanol/benzene extraction was the highest in the infrared spectrum of 400 cm-800 cm, 2750 cm-3200 cm wave number. In the 1750 cm-2400 cm wave segment, methanol, ethyl acetate and ethanol/benzene after the extraction of Pterocarpus pedatus Pierre powder infrared transmittance increased values are basically the same. GC-MS analysis, the health care ingredients in the Pterocarpus pedatus Pierre have cough and phlegm, heat detoxification, enhance human immunity, analgesic and anti-inflammatory and so on. Among them, Homopterocarpin is excellent in inhibiting and killing cancer cell activity; Cryptomeridiol is a natural product with anti-Alzheimer's disease and antispasmodic nature, and its medicinal value is remarkable. Scoparone has a wide range of pharmacological values.

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A Reverse transcription-polymerase chain reaction (RT-PCR) based detection of foot-and-mouth disease in District Faisalabad, Pakistan during the Year 2016

Cited by 13 publications

References 18 publications

Comparative Diagnosis of Typhoid Fever by Polymerase Chain Reaction and Widal Test in Southern Districts (Bannu, Lakki Marwat and D.I.KHAN) of Khyber Pakhtunkhwa, Pakistan

Comparative Diagnosis of Typhoid Fever by Polymerase Chain Reaction and Widal Test in Southern Districts (Bannu, Lakki Marwat and D.I.KHAN) of Khyber Pakhtunkhwa, Pakistan

Grup VII (Sind-08) Serotip Asya-1 Şap Hastalığı Virüsünün P1 Kodlayan Bölge Çeşitliliği

GC–MS explores the health care components in the extract of Pterocarpus pedatus Pierre

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