A Reusable Column Method Using Glycopolymer‐Functionalized Resins for Capture–Detection of Proteins and Escherichia coli

Abstract

The use of glycopolymer‐functionalized resins (Resin–Glc), as a solid support, in column mode for bacterial/protein capture and quantification is explored. The Resin–Glc is synthesized from commercially available chloromethylated polystyrene resin and glycopolymer, and is characterized by fourier transform infrared spectroscopy, thermogravimetry, and elemental analysis. The percentage of glycopolymer functionalized on Resin–Glc is accounted to be 5 wt%. The ability of Resin–Glc to selectively capture lectin, Concanavalin A, over Peanut Agglutinin, reversibly, is demonstrated for six cycles of experiments. The bacterial sequestration study using SYBR (Synergy Brands, Inc.) Green I tagged Escherichia coli/Staphylococcus aureus reveals the ability of Resin–Glc to selectively capture E. coli over S. aureus. The quantification of captured cells in the column is carried out by enzymatic colorimetric assay using methylumbelliferyl glucuronide as the substrate. The E. coli capture studies reveal a consistent capture efficiency of 105 CFU (Colony Forming Units) g−1 over six cycles. Studies with spiked tap water samples show satisfactory results for E. coli cell densities ranging from 102 to 107 CFU mL−1. The method portrayed can serve as a basis for the development of a reusable solid support in capture and detection of proteins and bacteria.

Glycopolymers for Antibacterial and Antiviral Applications

“…Similarly, Hong et al prepared magnetic iron oxide nanoparticles immobilized with mannose−based poly(ionic liquid) brushes for the efficient capturing of bacteria, and the nanocomposites also showed expedient recycling capacity and low cytotoxicity [ 25 ]. Ajish et al prepared a glucose−based polymer, which was modified on the resin surface [ 26 ] ( Figure 3 ). The “glycocluster effect” presented by the glucose on the resin surface was used to specifically bind Escherichia coli or ConA, making the functional resin available for capturing and quantifying Escherichia coli or ConA.…”