A retrospective evaluation of blood cultures in a pediatric intensive care unit: a three year evaluation

Abstract

Aim: In this study, it was aimed to retrospectively assess the frequency and antibiotic resistance of microorganisms isolated from blood cultures of patients in a pediatric intensive care unit. Material and Methods: The study was conducted on blood culture tests obtained from patients in a pediatric intensive care unit and sent to a microbiology laboratory between 2013 and 2016. The species and antibiotic susceptibilities were assessed in microorganisms isolated from the blood cultures. Results: Overall, 4239 blood cultures were obtained. Growth was detected in 324 blood cultures (7.6%). Of the microorganisms isolated, 195 (60.2%) were Gram-positive bacteria, and 107 (33.0%) were Gram-negative bacteria; 22 (6.8%) were fungi. The most commonly isolated microorganisms were Coagulase-negative staphylococci (45.1%), followed by Klebsiella pneumonia (14.5%), and Enterococcus faecalis (6.5%). Among the fungi, the most common was Candida albicans (59.1%), followed by Candida parapsilosis. The resistance rate against methicillin was 89.9% in coagulase-negative staphylococci, and 66% in S. aureus strains. The resistance rate against vancomycin was 3.6% in Enterococci spp. There was no resistance against linezolid in Gram-positive microorganisms. The rate of extended-spectrum beta lactamase positivity was found as 34% in Klebsiella spp. and 100% in Escherichia coli. The resistance rate against carbapenem was 44.9% in Gram-negative bacteriae. The resistance rate against carbapenem was 100% in Acinetobacter baumanii. In Candida albicans, resistance to amphotericine B was 61.5%, and resistance to voriconazole was 7.7%. Conclusions: To plan effective empiric antibiotic therapy against nosocomial infections in intensive care units, all units should have information about the characteristics of their own flora.

Prevalence, Antimicrobial Susceptibility Patterns, and Risk Factors Associated with Enterococci among Pediatric Patients at Dessie Referral Hospital, Northeastern Ethiopia

“…Similar findings were also reported in Ethiopia of Jimma (2.23%) [14], Addis Ababa (2.23% and 1.82%) [22,23] and Gondar (2.13%) [24]. However, it was lower than the previous reports from Turkey 6.5% [25], India 6% [26], and Gondar 8.9% [16]. The differences with the findings of this study may be attributed to the variation in geographical area, number of samples examined, the extent of time, and method used for sampling.…”

Caracterización de pacientes pediatricos con hemocultivos positivos del servicio de cuidado intensivo pediátrico del Hospital San José Bogotá, abril 2012 a 2017

“…El germen más frecuentemente aislado en hemocultivos contaminados fue S. epidermidis, similar a lo reportado en 2017 por Betül Ergül, de una serie de 3 años en una unidad pediátrica en Turquía 15 , donde reportaron un crecimiento del 7,6% de los hemocultivos tomados, con 45% de aislamiento de Staphylococcus coagulasa negativo que usualmente representan contaminación. Es importante a resaltar que el contaminante del hemocultivo en 4 casos fue un Staphylococcus aureus, en suelen ser patógenos y su determinación como germen contaminante requiere el contexto del paciente para detectarlos 16 .…”

Retrospective Evaluation of Vancomycin Resistant Enterococci Outbreak in Neonatal Intensıve Care Unit

“…In a study by Ertürk et al [18] , these rates were found to be 25% and 50%, respectively. In yet another study, 67.9% of enterococci showed HLGR and 67.9% showed HLSR [19] . In the present study, three patients exhibited both gentamicin and streptomycin resistance, but one was not analyzed for aminoglycoside sensitivity.…”