A rest period does not affect in vitro storage properties in apheresis platelets collected from the buffy coat layer

Abstract

BACKGROUND: A previous study demonstrated that several in vitro storage properties of apheresis platelets (PLTs) that are isolated by sedimentation against the collection container and subsequently resuspended can benefit from a rest period before continuous agitation. This study examines whether the in vitro storage properties of apheresis PLTs isolated by collection from the buffy coat layer benefit from a rest period before agitation. STUDY DESIGN AND METHODS: Freshly collected apheresis PLTs (Trima, GambroBCT) were divided into five 60‐mL aliquots. One aliquot was immediately placed on a flat‐bed agitator; the other aliquots were held on a laboratory bench for 1, 2, 4, and 6 hours before continuous agitation. Samples were obtained on Days 1, 5, and 7 for standard in vitro PLT assays. The experiment was repeated 12 times. RESULTS: For each sampling day, no significant differences were observed in aliquots held with or without a rest period for any of the following PLT properties: PLT content, mean PLT volume, pH, pCO2, bicarbonate, glucose, lactate, hypotonic shock response, extent of shape change, aggregation, morphology, CD62P, CD63, and CD42b. Although regression analysis identified several in vitro properties whose mean levels appeared to improve with increasing length of the rest period, maximum differences in mean levels were small (<6%). CONCLUSION: The in vitro storage properties of Trima apheresis PLTs isolated from the buffy coat layer do not benefit from a rest period.

Single Needle Granulocyte and Monocyte Apheresis for Ulcerative Colitis: A Retrospective Safety Analysis

“…Recently, several studies have demonstrated comparable effects of SN hemodialysis and DN hemodialysis in patients with chronic kidney disease without any adverse effects [10,11]. Further, platelet collection is similar in quantity and quality be-tween SN plateletpheresis and conventional DN plateletpheresis cl [17,18]. It has also been reported that SN-intensive GMA might be an adequate and novel therapeutic option for active UC as an alternative therapy before the initiation of corticosteroids [8].…”

Safety and efficacy of single‐needle leukocyte apheresis for treatment of ulcerative colitis

“…14,19 For platelet apheresis, reports have shown that the quantity and quality of obtained platelets were comparable for the SN and DN methods. 15,20 Conventional leukocyte apheresis with the DN method has been established as a safe and effective treatment strategy for active UC in Japan. 21 Nevertheless, the most important problems of leukocyte apheresis with DN method in clinical practice are related to needle puncture, thereby increasing the patient burden.…”

“…The limitation of SN hemodialysis is the inadequacy of treatment owing to lower blood flow, higher recirculation, and shorter treatment time, with suboptimal volume of cleared blood volume 14,19 . For platelet apheresis, reports have shown that the quantity and quality of obtained platelets were comparable for the SN and DN methods 15,20 …”