A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources

Muralidhar, R. V.; Chirumamila, RR; Marchant, Roger; Nigam, Poonam Singh Nee

doi:10.1016/s1369-703x(01)00117-6

Cited by 466 publications

(189 citation statements)

References 24 publications

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“…The maximal lipase yield by Penicillium roqueforti obtained at pH 5.5 [46]. While, Saccharomyces lipolytica produce maximal yield of lipase at pH 9.5 [47]. In this study the maximum enzyme activity was found to occur at pH 7, as reported in Fig.…”

Section: Effect Of the Ph Valuessupporting

confidence: 66%

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

El-Batal

Farrag

Elsayed

et al. 2016

ILNS

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Abstract. The present investigation was carried out to evaluate the effect of different growth conditions on lipase production by Aspegillus niger. The extracellular lipase producing fungus was isolated from spent bleaching earths. Optimizations of physical and chemical parameters were done for maximum lipase production using this isolate. Growth of the organism and lipase production were measured usig varying pH (4 -9), incubation temperature (20 -30 °C), incubation time (8 -80 hrs.), carbon sources, nitrogen sources, and shaking speed. Enhanced lipase production was observed at 24 °C, pH 7 and after 72hrs of incubation. Olive oil 5 % was observed as the most effective carbon source and Yeast extract 1.0 % as the most effective nitrogen source for lipase production. The optimum shaking value to get maximum lipase activity by Aspergillus niger was 200 rpm.

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Section: Effect Of the Ph Valuessupporting

confidence: 66%

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

El-Batal

Farrag

Elsayed

et al. 2016

ILNS

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“…The P values are used to check the significance of each coefficient, which in turn may indicate the pattern of the interaction between the variables (Zhang and Xue 2011). The smaller was the value of P, the more significant was the corresponding coefficient (Muralidhar et al 2001).It can be seen from this table that the linear coefficients (x 1 , x 2 and x 4 ), a quadratic term coefficient (x 2 1 ; x 2 3 and x 2 4 ) and cross product coefficients (x 1 x 3 , x 1 x 4 , x 2 x 3 and x 3 x 4 ) were significant, with very small P values (P<0.05). The results of the study showed that extraction temperature was the most significant single parameter which influenced the yield of AMP, followed by extraction time, alkali concentration and solvent/material.…”

Section: Statistical Analysis and The Model Fittingmentioning

confidence: 99%

“…The shapes of the contour plots, circular or elliptical, indicate whether the mutual interactions between the variables are significant or not. Circular contour plot indicates that the interactions between the corresponding variables are negligible, while elliptical contour plot indicates that the interactions between the corresponding variables are significant (Muralidhar et al 2001). …”

Section: Optimization Of Extraction Conditions Of Ampmentioning

confidence: 99%

Alkali extraction and in vitro antioxidant activity of Monascus mycelium polysaccharides

et al. 2012

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In the present work, alkali extraction technology was used to optimize the extraction of Monascus mycelium polysaccharides for the first time. The extracting parameters of alkali extracted Monascus mycelium polysaccharides were optimized by Box-Behnken design (BBD). The optimum conditions were extraction temperature 49°C, alkali concentration 7%, solvent/material ratio 23:1 (ml/g) and extraction time 2.3 h with an enhanced yield of 10.1%, compared with the yield 4.76% of hot water extraction, indicating that alkali extraction is a more efficient way. In order to discuss the biological activity of alkali extracted polysaccharides, we compared the in vitro antioxidant activity of alkali extracted polysaccharides (AMP) with hot water extracted polysaccharides (HMP). The result showed that AMP have the similar capability of scavenging both superoxide radical and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical of HMP in vitro. Therefore, alkali extraction technology is not only a highefficiency way to extract AMP, but also can retain the natural antioxidant activities of AMP, which can be used in pharmaceutical and food industries.

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“…Two variables within the experimental range were depicted in 3D surface plots while the other variable was kept constant at zero level. The shapes of the contour plots, circular or elliptical indicated whether the mutual interactions between the variables were significant or not (Liang et al 2010;Muralidhar et al 2001).…”

Section: Resultsmentioning

confidence: 99%

Optimization of extraction conditions for phytic acid from rice bran using response surface methodology and its antioxidant effects

Zhang

Bai

2011

J Food Sci Technol

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Solid-liquid extraction of phytic acid (PA) from rice bran was optimized by the maximization of the yield using response surface methodology. A Box-Behnken design was used to monitor the effects of three processing parameters of extraction on the PA yield, including ratio of acid solution to raw material (mL/g), hydrochloric acid concentration (mol/L), and extraction time (h). The results showed that the optimal conditions were acid solution/raw material of 8.5:1 (mL/g), HCl concentration 0.62 mol/L and extraction time 5.5 h. Validation tests indicated that the actual yield of PA was (2.15± 0.02)% with RSD=1.92% (n=5) under the optimized conditions, which was in good agreement with predicted yield. Antioxidant assays suggested that the extracted PA had weaker DPPH, hydroxyl and superoxide free-radical-scavenging capabilities than vitamin C at the same concentration of 0.5 mg/mL.

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A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources

Cited by 466 publications

References 24 publications

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

Alkali extraction and in vitro antioxidant activity of Monascus mycelium polysaccharides

Optimization of extraction conditions for phytic acid from rice bran using response surface methodology and its antioxidant effects

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