A residue-free green synergistic antifungal nanotechnology for pesticide thiram by ZnO nanoparticles

Abstract

Here we reported a residue-free green nanotechnology which synergistically enhance the pesticides efficiency and successively eliminate its residue. We built up a composite antifungal system by a simple pre-treating and assembling procedure for investigating synergy. Investigations showed 0.25 g/L ZnO nanoparticles (NPs) with 0.01 g/L thiram could inhibit the fungal growth in a synergistic mode. More importantly, the 0.25 g/L ZnO NPs completely degraded 0.01 g/L thiram under simulated sunlight irradiation within 6 hours. It was demonstrated that the formation of ZnO-thiram antifungal system, electrostatic adsorption of ZnO NPs to fungi cells and the cellular internalization of ZnO-thiram composites played important roles in synergy. Oxidative stress test indicated ZnO-induced oxidative damage was enhanced by thiram that finally result in synergistic antifungal effect. By reducing the pesticides usage, this nanotechnology could control the plant disease economically, more significantly, the following photocatalytic degradation of pesticide greatly benefit the human social by avoiding negative influence of pesticide residue on public health and environment.

Comparative Study on the Fungicidal Activity of Metallic MgO Nanoparticles and Macroscale MgO Against Soilborne Fungal Phytopathogens

“…Generally, the inactivation effects of nanoparticles are influenced by aggregation conditions, geometry, size, and physical properties (Herd et al, 2013). Several reports have studied metal nanoparticles that physically covered and penetrated the bacterial cell wall and revealed a tendency different from that of their microscale aggregates, such as SiO 2 and Al 2 O 3 (Jiang et al, 2009;Xue et al, 2014;Rodriguez-Gonzalez et al, 2016). It seems that the mechanistic interfacial interaction between the biological membrane and nanoparticle is crucial to understand the underlying mechanism (Krishnamoorthy et al, 2012;Sharma et al, 2015).…”

“…A series of inorganic and organic nanomaterials have been developed and proven to exhibit prominent antibacterial, antifungal, and antiviral properties on phytopathogenic microbes in vitro, and some of them still exerted their toxicity effects under greenhouse and field conditions. To date, TiO 2 , CuO (Hao et al, 2017(Hao et al, , 2019Liu et al, 2017), Zn, ZnO (Xue et al, 2014;Antonoglou et al, 2018;Sun et al, 2018), carbon nanomaterials (Chen et al, 2014(Chen et al, , 2016b, Al, and Si nanoparticles (Park H.J. et al, 2006;Shenashen et al, 2017) have been reported to display toxicity toward phytopathogenic bacteria and fungi, decreasing the disease incidence.…”

Preparation of ZnO/MWCNT/PP composite film and its application as multifunctional protective film

“…Previous work by Xue et al suggests that generation of reactive oxygen species (ROS) is responsible for the antimicrobial activity of ZnO nanoparticles. The generation of ROS such as hydrogen peroxide (H 2 O 2 ), hydroxyl radical ( · OH), superoxide ( ) radical induces oxidative stress on the cell membranes which causes damage to the cell wall, nucleic acids, and cellular proteins causing cell death of fungal hyphae . Wang et al studied antifungal property of SWCNT and MWCNT.…”

Nanobiofungicides: is it the next-generation of fungicides?

“…Few researchers have studied the combined antimicrobial effect of inorganic NPs with bioorganic pesticides in the field of plant protection, let alone demonstrated synergistic effect [21][22] . The effect of combination between different active ingredients may increase antimicrobial activity, reduce pesticide usage and delay the development of fungal resistance [23][24] .…”