A reproducible, rapid and inexpensive Folin–Ciocalteu micro-method in determining phenolics of plant methanol extracts

Cicco, Nunzia; Lanorte, Maria T.; Paraggio, Margherita; Viggiano, Mariassunta; Lattanzio, Vincenzo

doi:10.1016/j.microc.2008.08.011

Cited by 303 publications

(196 citation statements)

References 13 publications

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“…8,9 The maximum absorption of this chromatophore depends on the pH of the solution and the concentration of phenolic compounds. 8 Cicco et al 10 considered this to be a generalized technique, because some specific details may be modified, 9,11 such as the amount of Folin-Ciocalteu reagents and the sequence in which they are added, sodium-carbonate concentration, wavelength, the standard used, time period, and temperature. Modifications of this method are found in several pharmacopeias [12][13][14] and laboratory routines.…”

Section: Introductionmentioning

confidence: 99%

“…Modifications of this method are found in several pharmacopeias [12][13][14] and laboratory routines. 10,15,16 Considering the variations in this method, the present study aimed to develop a standard procedure for the pre-analytical method and analytical validation for the determination of total polyphenols in the crude extract from Caesalpinia peltophoroides bark.…”

Section: Introductionmentioning

confidence: 99%

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Development of a UV/Vis spectrophotometric method for analysis of total polyphenols from Caesalpinia peltophoroides Benth

Bueno¹,

Machareth²,

Panizzon³

et al. 2012

Quím. Nova

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Recebido em 23/8/11; aceito em 3/11/11; publicado na web em 4/1/12Caesalpinia peltophoroides is a domesticated tree found in Brazil. It was necessary to develop an analytical method to determine the content of total polyphenols (TP) in this herbal drug. The pre-analytical method was standardized for analysis time, wavelength, and the best standard to use. The optimum conditions were: pyrogallol, 760 nm, and 30 min respectively. Under these conditions, validation by UV/Vis spectrophotometry proved to be reliable for TP of the crude extract and semipurified fractions from C. peltophoroides. Standardization is required for every herbal drug, and this method proved to be linear, precise, accurate, reproducible, robust, and easy to perform.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a UV/Vis spectrophotometric method for analysis of total polyphenols from Caesalpinia peltophoroides Benth

Bueno¹,

Machareth²,

Panizzon³

et al. 2012

Quím. Nova

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“…The results were expressed in milligrams of ascorbic acid/100 g of fresh sample. The polyphenols assay was performed following the spectrophotometric method using the Folin Ciocalteu (Cicco et al, 2009) in which gallic acid was adopted as standard polyphenol. The results were expressed in milligrams of gallic acid/100g of sample.…”

Section: Discussionmentioning

confidence: 99%

Centesimal composition and bioactive compounds in African mustards used as condiments in Ivory Coast

Camara¹,

Soro²,

Traoré³

et al. 2016

Afr. J. Food Sci.

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The aim of this research was to evaluate the potential of African locust bean mustard, produced with fermented Parkia biglobosa seed and Glycine max seed, as functional food, focusing on its incorporation into the diet. These mustards contained in iron and zinc are respectively 12.16±0.63 mg/100 g and 8.38±0.3 mg/100 g in African locust bean mustard, and respectively 7 87±0.45 mg/100 g and 4.26±1.07 mg/100 g in soy mustard. Polyphenols and flavonoids were contained in large amounts. Ascorbic acid content was, respectively, 19.31 mg/100g of African locust bean mustard and 13.26 mg/100 g of soybean mustard. However, total carotenoids were 60.72±5.06 mg/100 g of African locust bean mustard and 100.86±8.45 mg/100g of soybean mustard. The lipid fraction contained unsaturated fatty acids in large amounts. Based on the results obtained, it can be said that West African mustards are excellent sources of vegetable proteins, iron, zinc, ascorbic acid, total carotenoids and oils rich in unsaturated fatty acids. These condiments may contain bioactive compounds with functional activities, but further research is needed to assess such potential.

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“…The phenolate ions reduce the yellow molybdotungstophosphate heteropolyanion into blue molybdotungstophosphate, which can be measured spectrophotometrically. 22,23 Based on statistical analysis using one-way ANOVA, it's known that there are no significant changes in total phenolic content for sample after irradiation at 5 and 7.5 kGy. The increase of phenolic content could be attributed to hydrolysis of glycosidic components by radical products from water radiolysis, leading to increasing of free phenolic compound.…”

Section: Total Phenolic Contentmentioning

confidence: 99%

Effect of Gamma Irradiation on Suruhan (Peperomia pellucida (L.) Kunth) Herb Powder

Yusuf¹,

Intan²,

Amelia³

et al. 2017

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Introduction: Peperomia pellucida (L.) Kunth is known as a raw material for herbal medicine; Preservation of herbs powder by gamma irradiation is reported to be able to keep products free from contamination. Methods: This study aims to evaluate the effect of gamma irradiation (0; 2.5; 5; 7.5; and 10 kGy) on the ACE inhibitory activity (ACE Kit -WST test kit method), antioxidant activity (DPPH radical scavenging method), total phenolic content (colorimetric method using Folin-Ciocalteu reagent), total flavonoid content (colorimetric method using AlCl3 and sodium acetate), and TLC profiling (silica gel F 254 as the stationary phase and dichlormethane:methanol [92:8] as the mobile phase) of suruhan herb powder. Results: Results showed that the 2.5 kGy irradiation dose gave the smallest alteration in ACE inhibitory activity compared to others irradiated doses. Furthermore, the 5 and 7.5 kGy dose didn't cause significant change (p>0.05) on antioxidant activity, total phenolic content, and total flavonoid content. Antioxidant activity was found to correlate with the total phenolic content but not with the total flavonoid content. Conclusion: Based on these finding, it is concluded that gamma irradiation can be used as a preservation method for P. pellucida herb powder.

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A reproducible, rapid and inexpensive Folin–Ciocalteu micro-method in determining phenolics of plant methanol extracts

Cited by 303 publications

References 13 publications

Development of a UV/Vis spectrophotometric method for analysis of total polyphenols from Caesalpinia peltophoroides Benth

Development of a UV/Vis spectrophotometric method for analysis of total polyphenols from Caesalpinia peltophoroides Benth

Centesimal composition and bioactive compounds in African mustards used as condiments in Ivory Coast

Effect of Gamma Irradiation on Suruhan (Peperomia pellucida (L.) Kunth) Herb Powder

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