Effect of Gamma Irradiation on Suruhan (Peperomia pellucida (L.) Kunth) Herb Powder

Abstract

Introduction: Peperomia pellucida (L.) Kunth is known as a raw material for herbal medicine; Preservation of herbs powder by gamma irradiation is reported to be able to keep products free from contamination. Methods: This study aims to evaluate the effect of gamma irradiation (0; 2.5; 5; 7.5; and 10 kGy) on the ACE inhibitory activity (ACE Kit -WST test kit method), antioxidant activity (DPPH radical scavenging method), total phenolic content (colorimetric method using Folin-Ciocalteu reagent), total flavonoid content (colorimetric method using AlCl3 and sodium acetate), and TLC profiling (silica gel F 254 as the stationary phase and dichlormethane:methanol [92:8] as the mobile phase) of suruhan herb powder. Results: Results showed that the 2.5 kGy irradiation dose gave the smallest alteration in ACE inhibitory activity compared to others irradiated doses. Furthermore, the 5 and 7.5 kGy dose didn't cause significant change (p>0.05) on antioxidant activity, total phenolic content, and total flavonoid content. Antioxidant activity was found to correlate with the total phenolic content but not with the total flavonoid content. Conclusion: Based on these finding, it is concluded that gamma irradiation can be used as a preservation method for P. pellucida herb powder.

Isolation of Steroid Compounds from Suruhan (Peperomia pellucida L. Kunth) and Their Antimalarial Activity

“…Characterization compounds 1 and 2: Different spectroscopic methods were used to elucidate the structure of isolated compounds 1 and 2. Among the spectroscopic techniques IR, 1 H and 13 C NMR, HMQC, HMBC and H-H COSY were carried out. The infrared spectrum was recorded on Shimadzu affinity-1, 1 H and 13 C NMR spectra were recorded using CDCl3 as solvent on JEOUL NMR 500 MHz spectrometer, Lembaga Ilmu Pengetahuan (LIPI), Indonesia.…”

REVIEW: AKTIVITAS ANTIOKSIDAN EKSTRAK HERBA SURUHAN (Peperomia Pellucida (L.) Kunth) DITINJAU DARI BEBERAPA METODE

“…Senyawa flavonoid merupakan senyawa metabolit sekunder golongan polifenol yang berperan sebagai antioksidan dengan penangkalan senyawa radikal bebas (Rhaman et al, 2019). Flavonoid merupakan senyawa yang bersifat cenderung polar karena memiliki gugus hidroksil, sehingga dapat larut dalam metanol, aseton, etanol, dimetil sulfoksida, dan butanol (Yusuf et al, 2017). Kandungan flavonoid pada tanaman berkontribusi dalam pemberi pigmen kuning, merah, oranye, dan ungu pada bagian buah, bunga, dan daun (Yunarto et al, 2018).…”

“…Tanin berfungsi sebagai antioksidan karena kemampuannya dalam menstabilkan fraksi lipid dan keaktifannya dalam penghambatan lipoksigenase (Okoh et al, 2017). (Yusuf et al, 2017). Reagen Folin-Ciocalteu mengandung asam fosfomolibdat dan asam fosfotungstat yang akan tereduksi oleh senyawa polifenol dan membentuk senyawa molybdenumtungsten, suatu kompleks berwarna ungu kebiruan (Ahmad dkk, 2019).…”

“…AlCl3 membentuk kompleks asam yang stabil dengan gugus keto (C-4) dan dengan gugus hidroksil C-3 atau C-5 dari flavon dan flavonol. Natrium asetat mendeteksi gugus hidroksil bebas di C-7 (Yusuf et al, 2017). Kadar flavonoid yang diperoleh hasilnya didapat sebagai mg ekuivalen kuersetin/g ekstrak (mgQE/g) (Rhaman et al, 2019).…”

Fungal Population and Aflatoxin Contamination on Stored Gamma-Irradiated Nutmeg (Myristica fragrans) Kernels

“…Gamma irradiation is a physical method that can be used to reduce microbial contamination and as an alternative treatment to chemical fungicides on agricultural commodities including crops, herbs, and spices. Presently the physical treatment can be used as preservation method [4] and has been developed and approved by national legislations in 0 over 60 countries worldwide and can be used to ensure food safety [5]. However, the sensitivity of fungi to irradiation depends on fungal strains [6].…”