A Remarkable Genetic Diversity of Rotavirus A Circulating in Red Fox Population in Croatia

Čolić, Daniel; Krešić, Nina; Mihaljević, Željko; Andreanszky, Tibor; Balić, Davor; Lolić, Marica; Brnić, Dragan

doi:10.3390/pathogens10040485

Cited by 9 publications

(12 citation statements)

References 32 publications

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“…RVA detection was performed on all samples collected for the present study, by the application of real-time RT-PCR that amplifies the fragment of VP2 segment of different RVA genotypes infecting humans and domestic animals ( Gutiérrez-Aguirre et al, 2008 ). Moreover, this protocol was successfully applied on wildlife ( Čolić et al, 2021 ) and water samples ( Steyer et al, 2011 ). Prior to one-step real-time RT-PCR, the RVA dsRNA was denaturated at 95 °C for 2 min together with primer mix (600 nM) and PCR grade water.…”

Section: Methodsmentioning

confidence: 99%

“…RT-PCR products were visualized by capillary electrophoresis on QIAxcel Advanced System (Qiagen, Germany) and subsequently purified using ExoSAP-IT™ PCR Product Cleanup Reagent (ThermoFisher Scientific, USA) as previously described ( Čolić et al, 2021 ). Purified PCR products were Sanger sequenced in both directions on 3730xl DNA Analyzer (ThermoFisher Scientific, USA) by Macrogen Europe (Amsterdam, the Netherlands).…”

Section: Methodsmentioning

confidence: 99%

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SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish

Brnić¹,

Lojkić²,

Škoko³

et al. 2022

Environmental Research

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The circulation of SARS-CoV-2 in the environment has been confirmed numerous times, whilst research on the bioaccumulation in bivalve molluscan shellfish (BMS) has been rather scarce. The present study aimed to fulfil the knowledge gap on SARS-CoV-2 circulation in wastewaters and surface waters in this region and to extend the current knowledge on potential presence of SARS-CoV-2 contamination in BMS. The study included 13 archive wastewater and surface water samples from the start of epidemic and 17 influents and effluents from nine wastewater treatment plants (WWTP) of different capacity and treatment stage, sampled during the second epidemic wave. From that period are the most of 77 collected BMS samples, represented by mussels, oysters and warty venus clams harvested along the Dalmatian coast. All samples were processed according to EN ISO 15216-1 2017 using Mengovirus as a whole process control. SARS-CoV-2 detection was performed by real-time and conventional RT-PCR assays targeting E, N and nsp14 protein genes complemented with nsp14 partial sequencing. Rotavirus A (RVA) real-time RT-PCR assay was implemented as an additional evaluation criterion of virus concentration techniques. The results revealed the circulation of SARS-CoV-2 in nine influents and two secondary treatment effluents from eight WWTPs, while all samples from the start of epidemic (wastewaters, surface waters) were negative which was influenced by sampling strategy. All tertiary effluents and BMS were SARS-CoV-2 negative. The results of RVA amplification were beneficial in evaluating virus concentration techniques and provided insights into RVA dynamics within the environment and community. In conclusion, the results of the present study confirm SARS-CoV-2 circulation in Croatian wastewaters during the second epidemic wave while extending the knowledge on wastewater treatment potential in SARS-CoV-2 removal. Our findings represent a significant contribution to the current state of knowledge that considers BMS of a very low food safety risk regarding SARS-CoV-2.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish

Brnić¹,

Lojkić²,

Škoko³

et al. 2022

Environmental Research

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“…Additionally, we cannot ignore the genetic diversity of RVA in rodents, which may impact the performance of the primers used in this study (Čolić et al 2021 ). Nevertheless, next-generation sequencing and/or primer-independent approaches are needed to improve the characterization of RVAs in the fecal samples of rodents and shrews in Bangladesh.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Group A rotavirus in rodents and shrews in Bangladesh

et al. 2022

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Rodents and shrews live in close proximity to humans and have been identified as important hosts of zoonotic pathogens. This study aimed to detect Group A rotavirus (RVA) and its potential risk factors in rodents and shrews in Bangladesh. We captured 417 small mammals from 10 districts with a high degree of contact between people and domestic animals and collected rectal swab samples between June 2011 and October 2013. We tested the swab samples for RVA RNA, targeting the NSP3 gene segment using real-time reverse transcription-polymerase chain reaction (rRT-PCR). Overall, RVA prevalence was the same (6.7%) in both rodents and shrews. We detected RVA RNA in 5.3% of Bandicota bengalensis (4/76; 95% CI: 1.4–12.9), 5.1% of B. indica (4/79; 95% CI: 1.4–12.4), 18.2% of Mus musculus (4/22; 95% CI: 5.2–40.3), 6.7% of Rattus rattus (6/90; 95% CI: 2.5–13.9), and 6.7% of Suncus murinus (10/150; 95% CI: 3.2–11.9). We found significantly more RVA in males (10.4%; OR: 3.4; P = 0.007), animals with a poor body condition score (13.9%; OR: 2.7; P = 0.05), during wet season (8.3%; OR: 4.1; P = 0.032), and in urban land gradients (10.04%; OR: 2.9; P = 0.056). These findings form a basis for understanding the prevalence of rotaviruses circulating among rodents and shrews in this region. We recommend additional molecular studies to ascertain the genotype and zoonotic potential of RVA circulating in rodents and shrews in Bangladesh.

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“…After the discovery of the rare G15 genotype in India [10], this genotype was reported in Argentina, Japan and Croatia [11][12][13][14][15][16]. So far, only 12 G15 genotype sequences have been deposited in the National Center for Biotechnology Information (NCBI) GenBank, all of which were bovine RVAs except for a fox and a human RVA strain [10][11][12][13][14][15][16]. Although the G15 genotype was found in 2021 in a fox in Europe [16], there are no reports yet on the presence of this genotype in other host species in European countries.…”

Section: Discussionmentioning

confidence: 99%

“…In the phylogenetic tree reconstructed with the nucleotide sequences of the VP7 gene (), strain K53 clustered with G15 RVA strains reported from Croatia [16] and Japan [11, 15]. After the discovery of the rare G15 genotype in India [10], this genotype was reported in Argentina, Japan and Croatia [11–16].…”

Section: Discussionmentioning

confidence: 99%

Whole-genome analysis of a rare G15P[21] group A rotavirus detected at a dairy cattle farm

Karayel-Hacioglu

Timurkan

Pellegrini

et al. 2022

Journal of General Virology

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Group A rotaviruses (RVAs) are a major cause of severe enteritis in humans and animals. RVAs have been identified in several animal species and their genetic diversity, the segmented nature of their RNA genome and the ability to spill over from one species to another can generate new RVA strains. In this study, we investigated the genome constellations of an unusual, rare, bovine RVA strain, G15P[21], identified from a farm with neonatal diarrhoea of calves in 2006. In parallel, the genome constellations of other RVA strains with different G/P types identified from the same farm in the same time span (2006–2008) were analysed. The genome constellation of strain K53 was G15-P[21]-I2-R2-C2-M2-A13-N2-T9-E2-H3 and was similar, overall, to that of the other bovine RVA strains (G6/10-P[11]-I2-R2-C2-M2-A13-N2-T6-E2-H3) with the exception of the NSP3 segment (T9 vs T6). This study describes RVA genomes with different genotype combinations isolated at a farm and also contributes to the understanding of the diversity and evaluation of rotavirus in a global context.

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A Remarkable Genetic Diversity of Rotavirus A Circulating in Red Fox Population in Croatia

Cited by 9 publications

References 32 publications

SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish

SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish

Epidemiology of Group A rotavirus in rodents and shrews in Bangladesh

Whole-genome analysis of a rare G15P[21] group A rotavirus detected at a dairy cattle farm

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