“…In addition to the above‐described in vitro RBC assays, additional quality assessments were performed after thaw according to CBS standard operating procedures. The assessment included calculation of RBC recovery, measurement of residual glycerol concentration by refractometry 38 (refractometer, Reichert, Depew, NY), measurement of total supernatant Hb (cyanmethemoglobin method 39 ), and sterility testing 40 (BacT/ALERT, Becton‐Dickinson Diagnostic Systems, Sparks, MD).…”