A regenerable piezoelectric immunosensor on the basis of electropolymerized polypyrrole for highly selective detection of Staphylococcal Enterotoxin A in foodstuffs

Karaseva, Nadezhda; Ermolaeva, T. N.

doi:10.1007/s00604-015-1456-1

Cited by 21 publications

(12 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dairy products, including milk, cheese, and yogurt have been frequently associated with SFP. [4c],[11b], To evaluate the effectiveness of the multiplexed strip for real samples, two naturally SEA contaminated, one SEC contaminated milk sample and pure milk samples spiked with different concentrations of the five SEs were analyzed. The naturally SEA and SEC contaminated milk samples were successfully detected, which showed good agreement with the ELISA method (SEA 15.6 and 8.1 ng mL −1 , SEC 12.5 ng mL −1 ).…”

Section: Resultsmentioning

confidence: 99%

“…Biosensors, including electrochemical sensors, quartz crystal microbalance (QCM) sensors, and fluorescence resonance energy transfer aptasensors, have been widely reported to be able to detect SEA or SEB, most of the time. [4c], The simultaneous detection of SEA and SEB using a QCM sensor and the simultaneous detection of SEA, SEB, and SED in milk using a immuno‐pillar device have been independently reported by Liu et al and Kasama et al, respectively. Furthermore, hydrogel‐based microarrays for the simultaneous detection of seven SEs (SEA, SEB, SEC1, SED, SEE, SEG, and SEI) have been reported by Rubina et al This biochip could detect the main SEs at a sensitivity of 0.1–0.5 ng mL −1 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gold‐Nanoparticle‐Based Multiplexed Immunochromatographic Strip for Simultaneous Detection of Staphylococcal Enterotoxin A, B, C, D, and E

Wang

Liu

et al. 2016

Part & Part Syst Charact

View full text Add to dashboard Cite

1wileyonlinelibrary.com www.particle-journal.com www. MaterialsViews.com Staphylococcal enterotoxins (SEs) in food samples can cause severe gastroenteritis even at low doses. Detection of SEs with a single assay would not only improve reliability and portability, but would also save time. In this paper, for the first time, a multiplexed gold-nanoparticle-based immunochromatographic (ICT) strip that allows the simultaneous detection of the five main SEs has been reported: SEA, SEB, SEC, SED, and SEE. Based on the sandwich immunoassay of each SE, a multiplexed ICT strip with five test lines is developed. Sensitive and specific paired monoclonal antibodies facilitate the detection, allowing for high sensitivity and good specificity. The portable biosensor platform can simultaneously detect SEA, SEB, SEC, SED, and SEE at concentrations as low as 2.5, 2.5, 2.5, 1, and 5 ng mL −1 , respectively. The entire test can be performed within 15 min and requires no sophisticated instruments except a portable strip reader. To evaluate the multiplexed strip, naturally SEA and SEC contaminated milk samples and pure milk samples spiked with the five SEs are analyzed.reportedly the second most common foodborne illness in the world. [1d,3] Among the family of identified SEs, the classical SEs, including SEA, SEB, SEC, SED, and SEE, account for 95% of SFP, and are therefore a concern in many countries. [4] The high rates of SE-related food poisoning are related to the pervasiveness of S. aureus and poor implementation of good manufacturing practices, resulting in the rapid growth of S. aureus and the subsequent production of the SEs; moreover, SEs can remain stable throughout food processing, and can thus still cause illness after the S. aureus was inactivated. [5] Furthermore, as a superantigen, the SEs can cause illness even at very low levels. It is reported that people who consumed three or more cartons of SEA contaminated chocolate milk (432 ng in average) have a high attack rate (44

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gold‐Nanoparticle‐Based Multiplexed Immunochromatographic Strip for Simultaneous Detection of Staphylococcal Enterotoxin A, B, C, D, and E

Wang

Liu

et al. 2016

Part & Part Syst Charact

View full text Add to dashboard Cite

show abstract

“…The detection of SEB was accomplished by monitoring the sensor’s fundamental resonance frequency. In addition, there are similar piezoelectric immunosensors for the detection of SEB [ 109 ] and SEA [ 110 , 111 ]. Magnetoelastic sensors are analogous to piezoelectric acoustic wave sensors, which measure parameters of interest by tracking the changes in their resonance behavior.…”

Section: Se Identification Using Immunoassaysmentioning

confidence: 99%

A Review of the Methods for Detection of Staphylococcus aureus Enterotoxins

Duan

et al. 2016

Toxins

136

View full text Add to dashboard Cite

Food safety has attracted extensive attention around the world, and food-borne diseases have become one of the major threats to health. Staphylococcus aureus is a major food-borne pathogen worldwide and a frequent contaminant of foodstuffs. Staphylococcal enterotoxins (SEs) produced by some S. aureus strains will lead to staphylococcal food poisoning (SFP) outbreaks. The most common symptoms caused by ingestion of SEs within food are nausea, vomiting, diarrhea and cramps. Children will suffer SFP by ingesting as little as 100 ng of SEs, and only a few micrograms of SEs are enough to cause SPF in vulnerable populations. Therefore, it is a great challenge and of urgent need to detect and identify SEs rapidly and accurately for governmental and non-governmental agencies, including the military, public health departments, and health care facilities. Herein, an overview of SE detection has been provided through a comprehensive literature survey.

show abstract

“…In the last decades, methods with the application of biosensors are gaining popularity (Eissa and Zourob 2012;Garibo et al 2014;Tang Alice et al 2002;Karaseva and Ermolaeva 2015;Justino et al 2015). Electrochemical, piezoelectric, and SPR sensors are developed for the detection of okadaic acid.…”

Section: Introductionmentioning

confidence: 98%

Highly Sensitive Detection of Okadaic Acid in Seafood Products via the Unlabeled Piezoelectric Sensor

2015

View full text Add to dashboard Cite

The affinity reaction between the antibodies to okadaic acid immobilized on the sensor's surface and the toxin in the solution has been investigated. The affinity constant, as well as the association rate constant and the dissociation rate constant, has been calculated. The unlabeled affinity sensor for the detection of okadaic acid has been developed. The calibration plot is linear in the range of concentrations of 5-500 ng mL −1 ; the limit of detection is 1.4 ng mL −1 . The developed sensor can be successfully used for the detection of toxins in real samples.

show abstract

A regenerable piezoelectric immunosensor on the basis of electropolymerized polypyrrole for highly selective detection of Staphylococcal Enterotoxin A in foodstuffs

Cited by 21 publications

References 15 publications

Gold‐Nanoparticle‐Based Multiplexed Immunochromatographic Strip for Simultaneous Detection of Staphylococcal Enterotoxin A, B, C, D, and E

Gold‐Nanoparticle‐Based Multiplexed Immunochromatographic Strip for Simultaneous Detection of Staphylococcal Enterotoxin A, B, C, D, and E

A Review of the Methods for Detection of Staphylococcus aureus Enterotoxins

Highly Sensitive Detection of Okadaic Acid in Seafood Products via the Unlabeled Piezoelectric Sensor

Contact Info

Product

Resources

About