A Refined Tissue Culture Medium for in Vitro Proliferation of Apple Rootstocks

Kermani, Maryam Jafarkhani; Hosseini, Zahra; Habashi, Ali Akbar; Geijskes, Robert J.; Pugalendhi, L.; Taji, Acram

doi:10.17660/actahortic.2009.829.48

Cited by 8 publications

(2 citation statements)

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“…Alternation in levels of endogenous IAA in the virusinfected stock shoots was most likely responsible for reduced length of shoots, while cell damage and alternations in mitochondria shape in the virusinfected shoots resulted in formation of abnormal shoots in cryopreserved shoot tips (Lloyd and McCown, 1980), y year(s), ZR zeatin riboside *Even though the term "calli" was used in the original, the term callus has been used here based on recommendation of Teixeira da Silva 2012bSilva 2010). Inclusion of 0.25% ascorbic acid, 0.5% citric acid and 3 g/l activated charcoal (AC) in the medium was found to reduce browning of apple rootstocks MM106 and MM111 (Jafarkhani Kermani et al 2009) and 'Douce de Djerba' (Boudabous et al 2010). Treatment of axillary and terminal buds in Murashige and Skoog (1962) medium (MS) containing 100 mg/l ascorbic acid reduced browning of apple rootstocks MM106, M7, MM111, M793 and M26 (Modgil and Thakur 2017).…”

Section: Conditions Nrmentioning

confidence: 99%

In vitro tissue culture of apple and other Malus species: recent advances and applications

Silva

Gulyás

Magyar-Tábori

et al. 2019

Planta

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Main conclusion Studies on the tissue culture of apple have allowed for molecular, biotechnological and applied breeding research to advance. In the past 8 years, over 100 papers advancing basic biology, genetic transformation and cryobiology have emerged. Apple (Malus × domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. In vitro tissue culture is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This updated review presents a synthesis of findings related to the tissue culture of apple and other Malus spp. between 2010 and 2018. Increasingly complex molecular studies that are examining the apple genome, for example, in a bid to identify the cause of epigenetic mutations and the role of transposable elements in this process would benefit from genetically stable source material, which can be produced in vitro. Several notable or curious in vitro culture methods have been reported to improve shoot regeneration and induce the production of tetraploids in apple cultivars and rootstocks. Existing studies have revealed the molecular mechanism underlying the inhibition of adventitious roots by cytokinin. The use of the plant growth correction factor allows hypothetical shoot production from leaf-derived thin cell layers relative to conventional leaf explants to be determined. This updated review will allow novices and established researchers to advance apple and Malus biotechnology and breeding programs.

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Section: Conditions Nrmentioning

confidence: 99%

In vitro tissue culture of apple and other Malus species: recent advances and applications

Silva

Gulyás

Magyar-Tábori

et al. 2019

Planta

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“…During the early spring, plant tissue cultures of red flesh apple trees M. niedzwetzkyana were initiated from donor plants grown in two regions in Iran: Shahrud (Semnan Province) and Aznab (Zanjan Province). Stem cuttings containing lateral buds were divided into pieces with length of 1.5 to 2.5 cm and surface-sterilized according to Jafarkhani Kermani et al (2009). The explants were placed in the test tubes containing Murashige and Skoog medium (MS) (Murashige & Skoog 1962) supplemented with 2 µM 6-benzylaminopurine (BAP).…”

Section: Plant Materials and General Proceduresmentioning

confidence: 99%

Sodium Nitroprusside Stimulates Micropropagation and TDZ Induces Adventitious Shoots Regeneration in Red Flesh Apple Malus niedzwetzkyana Koehne Dieck ex

Kazemi

Kermani

Habashi

2019

Journal of Horticultural Research

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The aim of the present investigation was to optimize protocols for micropropagation and adventitious shoot regeneration from leaf explants of two wild ecotypes of red flesh apple Malus niedzwetzkyana for future breeding programs. At the proliferation stage, different concentrations of sodium nitroprusside (SNP) and triacontanol (TRIA) were compared. To optimize shoot regeneration from leaf explants, interactive effects of 1-phenyl-3-(1,2,3-thidiazol-5-yl)-urea – thidiazuron (TDZ), indole-3-butyric acid (IBA) and two explant types were investigated. At rooting stage, the effect of exposure time of microshoots to darkness and exposure time to different concentrations of IBA and α-naphthalene acetic acid (NAA) were compared. The results showed that SNP affected the growth rate significantly and the maximum multiplication rates per explant (9.6 in the first ecotype and 8.8 in the second) were produced in the Quoirin and Lepoivre medium containing 17 SNP µM, in addition to 4 µm 6-benzylaminopurine (BAP) and 3 µm gibberellic acid (GA3). IBA and TDZ affected the adventitious shoot regeneration from leaf explants significantly, the highest number of regenerated shoots (18.3 per explant) was obtained from the basal section of the leaves cultured on the medium containing 2 μM IBA and 15 μM TDZ. At rooting stage, the maximum rooting (88.6%) was obtained in the result of one weak exposure to darkness on medium containing 3 μM IBA.

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Optimisation of the media forin vitroshoot proliferation and root induction in three new cold-hardy and dwarfing or semi-dwarfing clonal apple rootstocks

Sun

Bell

et al. 2014

The Journal of Horticultural Science and Biotechnology

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A Refined Tissue Culture Medium for in Vitro Proliferation of Apple Rootstocks

Cited by 8 publications

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In vitro tissue culture of apple and other Malus species: recent advances and applications

In vitro tissue culture of apple and other Malus species: recent advances and applications

Sodium Nitroprusside Stimulates Micropropagation and TDZ Induces Adventitious Shoots Regeneration in Red Flesh Apple Malus niedzwetzkyana Koehne Dieck ex

Optimisation of the media forin vitroshoot proliferation and root induction in three new cold-hardy and dwarfing or semi-dwarfing clonal apple rootstocks

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