A red line not to cross: Evaluating the limitation and properness of gel image tuning procedures

Wu, Heng; Yen, Chien-Chang; Tsui, Wen-Huei; Chen, Han-Min

doi:10.1016/j.ab.2009.08.041

Cited by 7 publications

(5 citation statements)

References 17 publications

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“…Therefore, they suggested image optimization before the analysis. Preprocessing of gel image was employed by all these gel reading applications (Bajla et al, 2005: Cooper et al, 1996: Cardinali et al, 2002, although Wu et al, (2010) highlighted the danger of adjusting the gel images because it can cause erroneous outputs. Only linear transformations are suggested not to deviate too much from the original image.…”

Section: Discussionmentioning

confidence: 99%

An automated system for analyzing agarose and polyacrylamide gel images

Abeykoon¹,

Dhanapala²,

Yapa³

et al. 2015

Ceylon J. Sci. (Biol. Sci.)

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Agarose or polyacrylamide gel electrophoresis is a common technique used to separate nucleic acids (DNA and RNA) according to their molecular weight, which generates images that can be analyzed automatically by a system. The separated DNA fragments of different molecular weights give a series of bands with positions in the gel image corresponding to their molecular weights. Automated image analysis of the gels removes much of the subjectivity of manual interpretation of band positions and sizes. In this paper, an automated system was designed for analyzing DNA bands using image and signal processing techniques. The proposed algorithm consists of four main steps: preprocessing to enhance the image, detecting lanes and bands, identifying band lengths and clustering; in which the similarities of biological samples based on shared bands can be identified. The proposed technique eliminates defects due to noise and double bands in images.

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Section: Discussionmentioning

confidence: 99%

An automated system for analyzing agarose and polyacrylamide gel images

Abeykoon¹,

Dhanapala²,

Yapa³

et al. 2015

Ceylon J. Sci. (Biol. Sci.)

View full text Add to dashboard Cite

show abstract

“…The bands were visualized using 0.001 M 4chloro1naphtol solution in 0.5 M Tris pH 7.5 and 0.03% H 2 O 2 . Concentrations of the cross linked γ-chains were calculated using densitometry of scanned electrophoregrams with TotalLab TL100 software (Nonlinear Dynamics, NewcastleUpon Tyne, UK) [18].…”

Section: Methodsmentioning

confidence: 99%

Thromboelastographic study of fibrin clot and molecular basis of maximum clot firmness

Korolova¹,

Stohnii²,

Gryshchuk³

et al. 2021

Ukr.Biochem.J

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“…Bland-Altman analysis: bei der Bewertung mit L340 konnten geringere Abweichungen von den Referenzwerten, als mit TL120 dargestellt werden (mean: -1.5 % ; SD: 23.0 % to -25.9 % ) vs. TL120 (mean: -8.2 % ; SD: 32.6 % to -48. 6 Schl ü sselw ö rter: 1D-Gelelektophorese; LabImage 1D (L340); TotalLab 120 (TL120).…”

Section: Zusammenfassungunclassified

Impact of image analysis software on quantitation of 1D gel electrophoresis analysis/Einfluss der Bildanalysesoftware auf die Bewertung von 1D Gelelektrophoresen

Kahlenberg

Sack

Boldt

2012

LaboratoriumsMedizin

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Background: Image analysis of 1D gel electrophoresis can be performed by numerous types of software. The results are used, e.g., to create reference data or for association with diseases. In this study, we analyzed statistical differences between two types of established image analysis software. The aim was to inform customers that different types of software may produce various results which may lead to different data interpretations. Methods: Automated serum protein electrophoresis (albumin, α 1 -globulin, α 2 -globulin, β -globulin and γ -globulin) was performed with sera from 25 patients (randomized). Gel bands were quantitatively analyzed by TotalLab 120 (TL120), LabImage 1D (L340) and Phoresis (reference standard). Finally, the degree of deviation (vs. reference standard) of obtained data was investigated by statistical methods (BlandAltman, Passing-Bablok, reliability). Results: Passing-Bablok analysis: in L340 and TL120 linearity of test data vs. reference data was passed (p < 0.01; L340: y = 0.00 + 1.00x vs. TL120: y = -0.01 + 1.02x). Bland-Altman analysis: L340 exhibited a lower deviation and standard deviation to reference (mean: -1.5 % ; SD: 23.0 % to -25.9 % ) vs. TL120 (mean: -8.2 % ; SD: 32.6 % to -48.6 % ). Reliability: L340 (k = 0.404; 95 % CI = 0.315 -0.493) vs. TL120 (k = 0.105; 95 % CI = 0.105 -0.245). Detailed serum protein analysis

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A red line not to cross: Evaluating the limitation and properness of gel image tuning procedures

Cited by 7 publications

References 17 publications

An automated system for analyzing agarose and polyacrylamide gel images

An automated system for analyzing agarose and polyacrylamide gel images

Thromboelastographic study of fibrin clot and molecular basis of maximum clot firmness

Impact of image analysis software on quantitation of 1D gel electrophoresis analysis/Einfluss der Bildanalysesoftware auf die Bewertung von 1D Gelelektrophoresen

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