A recombinant human RNASET2 glycoprotein with antitumorigenic and antiangiogenic characteristics

Abstract

Objectives: Diets consisting of diverse food items provide a wide range of nutrients that can enhance nutritional quality of the diet. Few studies have, however, assessed dietary diversity and its effects on micronutrient health in rural populations in field settings. This study assesses how well Dietary Diversity Score (DDS), an indicator of dietary diversity based on a simple count of food groups consumed, predicts the micronutrient status, focusing on serum vitamin A concentration. Methods: We used cross‐sectional data from women in food‐insecure northern Kenya where dietary diversity is likely critical for micronutrient health yet under‐studied. A linear regression model was applied to examine the relationships between DDS and serum retinol concentration. A logistic regression model was used to test DDS as a predictor of vitamin A insufficiency (serum retinol < 1.05 μmol/l). Results: DDS had a significant positive effect on serum retinol concentration (t = 2.01, P = 0.045) after adjusting for age, wealth, acute phase reaction, hemoglobin, vitamin A intake and vitamin A supplementation. A one unit increase in DDS by adding an extra food group in one's diet was significantly less likely to have vitamin A insufficiency (OR = 0.64, P = 0.026) after adjusting for the covariates. Conclusions: Our results indicate that diversified diets enhance vitamin A status relative to narrower diets with equivalent vitamin A content. DDS shows a potential as a low‐cost, field‐friendly method for exploratory assessments of vitamin A status, and a potential as a research tool for human biologists and anthropologists interested in dietary quality and micronutrient health. Am. J. Hum. Biol., 2012. © 2012 Wiley Periodicals, Inc.

RNASET2 in human spermatozoa and seminal plasma: a novel relevant indicator for asthenozoospermia

“…Their close association clearly suggested a potential correlation between RNASET2 and sperm motility. Furthermore, the actin-binding capability of RNASET2 in both human spermatozoa and seminal plasma was confirmed by immunoprecipitation and indirect ELISA, which agrees with previous reports in which their identity was established based on membrane blotting and a cross-linking assay Smirnoff et al, 2006).…”

tRNA biology charges to the front

“…However, the activity associated with Rny1 that mediates cell death is demonstrably separate from its cleavage activity (Thompson and Parker 2009a). Intriguingly, the human Rny1 ortholog RNASET2 has a set of activities similar to those of yeast Rny1, since expression of RNASET2 in yeast restores the production of tRNA cleavage fragments in an rny1 mutant strain and restores the reduced viability of cells undergoing oxidative stress (Thompson and Parker 2009a), while expression of RNASET2 in mammalian cells acts as a tumor suppressor independent of its cleavage activity (Acquati et al 2005;Smirnoff et al 2006). tRNA cleavage products are also generated by Dicerdependent cleavage.…”

Isolation of Pollen-expressed Actin as a Candidate Protein Interacting with S-RNase in Prunus avium L.

“…Actin was reported to bind to some of the T2/S-type RNase family proteins, such as human RNASET2 and Aspergillus ACTIBIND. E3 ligase, such as SLF in Plantaginaceae and SBP1 and SLF in Solananaceae, were also reported to bind to S-RNase (Hua and Kao, 2006;O'Brien et al, 2004;Qiao et al, 2004;Roiz et al, 2006;Sims and Ordanic, 2001;Smirnoff et al, 2006). Although E3 ligase homologs can be very good candidates for general inhibitors of S-RNase, we selected the actin homolog for further characterization in this study and left E3 ligase homologs for future studies, because we suspected that S-RNase may exert its cytotoxic effects through its actin-binding ability.…”

“…Both the F-and G-forms of actin were indicated to bind to reduced but not to non-reduced S-RNase. RNASET2 and ACTIBIND have been reported to exert cytotoxicity on human cancer cells regardless of their RNase activity Smirnoff et al, 2006); thus, their actin-binding ability was speculated to provide such a cytotoxic effect (Luhtala and Parker, 2010). Actin dynamics were suggested to play a central role in coordinating many aspects of pollen tube growth, i.e.…”