A rearranged genomic segment 11 is common to different human rotaviruses

Abstract

Gene 11 of human rotaviruses with short electropherotype, independently obtained from infected children in Argentina, have an insertion of 148 nt in the 3' untranslated region. All viruses were highly homologous among them and with two others human strains, DS-1 and RV5.

G8Rotavirus Strains Isolated in the Democratic Republic of Congo Belong to the DS-1-Like Genogroup

“…This larger gene size was due to an AT-rich insertion into the 3Ј end of the gene just behind the stop codon of the NSP5 protein. A very similar gene structure has been described previously for RV strains isolated in Argentina (v47, v51, v61, v115, v158, and v252), India (NR1), People's Republic of China (TB-Chen), Japan (KUN), Australia (RV-5), and the United States (DS-1) (23,39,47). Percentages of nucleotide identity between the DRC88 and DRC86 NSP5 genes and the NSP5 gene of these strains ranged from 89.6% for the Argentinean, Australian, and American strains to 97.1% for the Japanese, Chinese, and Indian strains.…”

“…A phylogenetic tree that includes the complete VP4 amino acid sequences of our DRC88 and DRC86 strains and all known P types (except for P [22], P [23], and P [25], because no complete sequence data were available) was constructed (Fig. 1C).…”

“…After the isolation of RV strains with an unusually large NSP5 gene, due to the insertion of a long noncoding AT-rich region in their 3Ј end, in Asia (China, Japan, India, and Australia), South America (Argentina), and North America (United States) (23,39,47), DRC88 and DRC86 are the first such HRV strains isolated and sequenced on the African continent. The first HRV strains with this rearranged NSP5 gene were isolated in 1977 (4, 32), and they have been isolated all over the world since then.…”

Rotavirus NSP5 phosphorylation is up-regulated by interaction with NSP2.

“…NSP5 is encoded in the longer ORF of genomic segment 11. It is a glycosylated phosphoprotein of 198 aa (in SA11) (Welch et al, 1989) with a high degree of conservation among different strains of group A rotaviruses including viruses with rearrangements of genome segment 11 (Gianbiagi et al, 1994). The NSP5 primary sequence is characterized by an overall high content (24 %) of serine and threonine.…”

Rearrangements of Rotavirus Genomic Segment 11 Are Generated during Acute Infection of Immunocompetent Children and Do Not Occur at Random

“…Although rotavirus strains with a rearranged segment 11 (11R) of unknown (5,12) or animal (20,23) origin have seldom been found to circulate among immunocompetent children, gene rearrangements have been detected mostly in vivo during chronic infection in immunodeficient children or in vitro during passages at a high MOI in cell culture. It is assumed that these unusual replication conditions lead to selective advantages favoring the recovery of viruses with rearranged genes.…”