A ratiometric fluorescent probe for detecting hypochlorite in the endoplasmic reticulum

Abstract

A colorimetric and fluorescent probe ER-ClO was developed to detect cellular hypochlorite with high selectivity and sensitivity.

Fluorescent probes for organelle-targeted bioactive species imaging

“…The ER is the main place for protein fabrication, modification, folding and transportation. It also takes charge of the synthesis of other molecules, like lipids and carbohydrates, as well as Ca 2+ storage 30b,96. During synthesis of diverse proteins, RONSS are indispensable for controlling their functional three-dimensional structures and chemical groups 50b,97.…”

Guiding Drugs to Target‐Harboring Organelles: Stretching Drug‐Delivery to a Higher Level of Resolution

“…[12][13][14] Imaging of the fluorescent molecule in combination with organelle-specific fluorescent molecular markers enables the determination of the subcellular distribution by analysis of colocalization patterns.Fluorescent imaging is relatively simple to implement, reliable,a nd allows real-time analysis of the subcellular distribution of the fluorescent molecules in live cells.O fn ote,f luorescence spectrum and quantum yield of afluorophore may vary depending on parameters such as pH, ionic strength, or binding to different targets. This ratio can be increased by directing the drug to the diseased tissue or pathogenic cell.…”

Reversible detection of hypochlorite using the deprotonation–protonation strategy: a search for new building blocks

“…4,5 Though the whole process is well regulated, however, a slight over production of hypochlorite may result in the accumulation of ClO À in living systems which eventually generates oxidative stress and hence can cause various neurodegenerative and cardiovascular diseases and cancer. [6][7][8] Over the last few years, a variety of reaction-based fluorescent probes involving irreversible changes around carbon-carbon, [9][10][11][12][13][14] carbon-nitrogen, 15,16 carbonsulphur, 17,18 and carbon-oxygen 19 bonds have been reported for the detection of hypochlorite (Table S1, ESI †). The reaction-based strategy is efficient but hypochlorite induced irreversible chemical changes are introduced in the probe as a result of which the probe is rendered useless after its first application.…”

“…Furthermore, the assemblies of probe 1 exhibit strong antioxidant potential for suppressing the sodium hypochlorite induced oxidative transformation of benzylamine under lab conditions. Although the applications of a variety of probes have been demonstrated for organelle target 11,14 cell imaging (Table S1, ESI †), the potential of none of these probes has been explored as an antioxidant for protection against hypochlorite induced oxidative stress. In this context, unprecedented, the work being presented in this manuscript demonstrates the potential of the assemblies of 1 to protect the living cells from hypochlorite induced cytotoxicity.…”