A ratiometric ESIPT fluorescent probe for detection of anticancer-associated H2O2 level in vitro and in vivo

Recent progress of organic fluorescent molecules for bioimaging applications: cancer-relevant biomarkers

“…33) was prepared by using a benzylborate pinalol ester group as the H 2 O 2 recognition unit. 82,83 The pyridinium structure on the skeleton of HBQ could not only realize long-wavelength emission through the ICT mechanism but also facilitate mitochondrial targeting. HBQ-L showed sensitivity and selectivity for H 2 O 2 detection through a greento-red fluorescence response.…”

“…These results suggested that HBQ-L could be a promising tool to reveal the precise role of H 2 O 2 . 82…”

An ESIPT-based ratiometric fluorescent probe for detecting H2O2 in water environment and biosystems

“…Various small molecule fluorescent probes have been designed for in vitro and in vivo detection of H 2 O 2 , including benzyl, boric acid ester, arylsulfonyl ester, etc. ( Chen et al, 2022 ; Gu et al, 2020 ; Guo et al, 2022 ). Previously reported H 2 O 2 fluorescence probes have some disadvantages such as single-wavelength, small Stokes shift, long response time, and low sensitivity ( Gao et al, 2017 ; Li et al, 2022b ).…”

A highly effective “naked eye” colorimetric and fluorimetric curcumin-based fluorescent sensor for specific and sensitive detection of H2O2in vivo and in vitro

“…Existing quantitative detection methods, such as titrimetry, electrochemistry, spectrophotometry and so on, are relatively mature techniques. 17–19 Fluorescent sensor analysis has the advantages of fast response, strong selectivity, adaptability and microscopic properties, and it has been widely used for detecting some analytes in biological and environmental systems. 20–23 At present, there are many reported sensors for H 2 O 2 which use aryl borate or phenyl boric acid reactions to generate phenol, phenol oxidation to give quinone, oxonium or diketone to give acid, carbon–carbon double bond breaking, and so on.…”