A rapid screening method for bacteria degrading polycyclic aromatic hydrocarbons

Abstract

Aims: A rapid procedure was developed to screen for bacteria that are able to grow on polycyclic aromatic hydrocarbons (PAHs). Methods and Results: A drop of ethyl ether‐dissolved PAH is spread on a sterilized cellulose acetate/nitrate filter lying on the top of a mineral salts agarose plate. After the evaporation of ethyl ether, a serially diluted sample is spread over the filter and incubated. Subsequently, the PAH degrading bacteria can be counted and isolated. Conclusions: This procedure is a simple method for screening bacterial isolates for the ability to grow with PAHs. Significance and Impact of the Study: This technique is rapid to screen and/or count PAH‐degrading bacteria and is also used to streak cultivation without disrupting the PAH layer on plate.

A Genomic Study to Explore the Coping Mechanisms of Bacteria to Hydrocarbon Stress

“…Genome Assembly. Screening of B[a]P degrading bacteria was done by spray plate technique (Zhao et al, 2009). The stock solution of B[a]P contained 100µg of B[a]P dissolved in 1 ml dichloromethane.…”

Isolation and Characterization of Hydrocarbon Degrading Bacteria from Petrol Contaminated Soil

“…The enumeration is the best way to study the PAHdegrading bacterial population [25]. Therefore, hydrocarbon degrading bacterial strains were enumerated by spread plate method on nutrient agar plate [21].…”