A rapid response near-infrared ratiometric fluorescent probe for the real-time tracking of peroxynitrite for pathological diagnosis and therapeutic assessment in a rheumatoid arthritis model

Abstract

Peroxynitrite (ONOO−) is a potent bio-oxidant involved in many physiological and pathological processes, however, most of the pathological effects associated with ONOO−in vivo are still ambiguous. Herein, we design and...

Quinoline-based fluorescent probe for the detection and monitoring of hypochlorous acid in a rheumatoid arthritis model

“…We then evaluated the capability of HQ for monitoring the RA treatment through assessing the HOCl-mediated fluorescence response in live mice. MTX, a standard therapeutic drug for RA patients, 52 was administered to left hind limb locally. As the control, the right hind limb was injected with same amount of PBS.…”

Recent Progress of Small‐Molecule Ratiometric Fluorescent Probes for Peroxynitrite in Biological Systems

“…By adjusting the recognition site to a diene unit, the You group fabricated two similar ratiometric fluorescent probes 18 a and 18 b (Figure 5) for ONOO À . [64] The superior probe 18 a was further used to visualize the ONOO À fluctuations in biological systems. The exogenous and endogenous ONOO À were easily visualized in live cells by ratio imaging and flow cytometry analysis.…”

“…Biological application demonstrated that probe 17 could anchor mitochondria and achieve ratiometric fluorescence imaging of ONOO − generation in live cells stimulated with LPS/IFN‐γ/PMA. By adjusting the recognition site to a diene unit, the You group fabricated two similar ratiometric fluorescent probes 18 a and 18 b (Figure 5) for ONOO − [64] . The superior probe 18 a was further used to visualize the ONOO − fluctuations in biological systems.…”

Activatable Rare Earth Near-Infrared-II Fluorescence Ratiometric Nanoprobes

“…For further bioapplication, the cellular toxicity of the probe had been evaluated by using a methyl thiazolyl tetrazolium (MTT) assay, which proved the negligible cytotoxicity of DSNPs@A (Figure S16). Encouraged by the above results, we used the DSNP@A for the detection of ONOO – in vivo in an autoimmune-induced RA mouse model (see the Supporting Information for experimental details), since ONOO – exhibited a relatively high concentration in the articular cavity of RA. − X-ray detection and H&E staining confirmed the formation of acute synovitis in the joint cavity, which accompanied by large amounts of ONOO – produced (Figure S17). Afterward, the DSNP@A (5 mg mL –1 , 25 μL) solution was administrated via intra-articular injection in RA mice with healthy mice as control.…”