A Rapid Method for Isolating Mutants of <i>Bacillus subtilis</i> Producing Increased or Decreased Amounts of the Antibiotic, Mycobacillin

Banerjee, Ayan; Bose, S. K.

doi:10.1111/j.1365-2672.1964.tb04816.x

Cited by 6 publications

(1 citation statement)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The plates containing the established colonies were then flooded with agar seeded with the mycobacillin-sensitive fungus Aspergillus niger G, Br to detect non-producer strains according to the method of Banerjee & Bose (1964). Two thousand colonies were screened and Mymutants thus identified were then transferred to nutrient broth containing 250 pg mycobacillin ml-' and incubated for 24 h at 30 "C. The blocked mutants, now free from A .…”

Section: E T H O D Smentioning

confidence: 99%

Accumulation of Peptides by Mycobacillin-negative Mutants of Bacillus subtilis B3

Majumder¹,

Ghosh²,

Mukhopadhyay³

et al. 1985

Microbiology

Self Cite

View full text Add to dashboard Cite

Thirteen mycobacillin-negative (My-) mutants of Bacillus subtilis B3 were isolated from an auxotrophically tagged mycobacillin producer organism. The wild-type producer, three feeble producers and three strictly My- mutants did not accumulate any ninhydrin-positive peptide in the culture medium while the remaining seven My- mutants did accumulate ten such peptides whose amino acid composition indicated that there might be only three different peptides. The N-terminal and C-terminal amino acid residues implicated one of these peptides as a pentapeptide intermediate in mycobacillin synthesis; this was further confirmed by its molecular weight and sequence. Studies on cell-free synthesis showed that only the enzyme system from the wild-type strain synthesized mycobacillin while the defective ones from all the My- mutants synthesized one and the same pentapeptide as found in the culture broth of some of the mutants. Further studies in which the enzymes responsible for mycobacillin synthesis by cell-free extracts were separated into three fractions, A, B and C, showed that seven of the mutants were defective in fraction B whereas the three other mutants had defects in both fractions B and C. Thus the pentapeptide Pro----Asp----Glu----Tyr----Asp appears to be implicated in mycobacillin biosynthesis.

show abstract

Section: E T H O D Smentioning

confidence: 99%