A Rapid Method for Estimating Oestrogens in Urine Using a Semi-Automatic Extractor

Brown, J. B.; MacLeod, S.C.; Macnaughtan, Caroline; Smith, Margery A.; Smyth, Barbara

doi:10.1677/joe.0.0420005

Cited by 417 publications

(77 citation statements)

References 16 publications

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“…Previous work (Genazzani et at., 1975) has shown that plasma cortisol and ACfH undergo cyclic changes during the menstrual cycle. In the present study the excretion, in urine, of free corticosteroids and total oestrogens was examined as indices of adrenal and ovarian activity, respectively, The oestrogen/creatinine ratio measurements showed the normal biphasic pattern associated with follicular growth and ovulation shown by previous studies of 24-hour excretion (Brown et al, 1968). The urinary free ll-hydroxycorticosteroid/ creatinine ratio showed random fluctuations through the follicular phase.…”

Section: Methodsmentioning

confidence: 50%

Excretory Patterns of Urinary Free 11-Hydroxycortico-Steroids and Total Oestrogens Throughout the Normal Menstrual Cycle

Walker

McGilp

1978

Ann Clin Biochem

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SUMMARY The excretion of urinary free ll-hydroxycorticosteroids and total oestrogens was studied daily in morning urine specimens throughout the menstrual cycle of six normal women (age range 18-24 years). The follicular phase of the cycle was characterised by apparently random fluctuations in the excretion of urinary free corticosteroids. However, after the mid-cycle oestrogen peak there occurred a significant drop in corticosteroid excretion, which then rose to a peak eight to 10 days after ovulation and was synchronous with the second oestrogen peak during the luteal phase.Results o 0 -10-9-8 -7-6-5-'.-3-2-1 0 1 2 3 L. 56 7 8910 §.Ooys relative 10 mid-cycle oestrogen peak (day 0)T he composite cycle shown in the Figure represents the mean excretion of urinary free corticosteroids and total oestrogens in six volunteer subjects. i10

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Section: Methodsmentioning

confidence: 50%

Excretory Patterns of Urinary Free 11-Hydroxycortico-Steroids and Total Oestrogens Throughout the Normal Menstrual Cycle

Walker

McGilp

1978

Ann Clin Biochem

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“…Twin pregnancies and those with doubtful duration were excluded. In the third trimester of pregnancy the following determinations were made: An average of 4 determinations of the biparietal head diameter using the B-scan method (Vidoson), 10 determinations of estriol and 3 of pregnandiol in urine [13,50] and 6 HPL determinations in the semm [49], 7 determinations of heat stable alkaline phosphatase (HSAP) [9], all per individual case. Biparietal head diameter values were transferred to a Standard curve that was in agreement with the one reported by HOLLÄNDER [42].…”

Section: Methodsmentioning

confidence: 99%

Ultrasound and biochemical findings in intrauterine growth retardation

Kunz¹,

Keller²

1976

Journal of Perinatal Medicine

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The early recognition of fetal maldevelopment due to chronic placental insufficiency is of decisive importance if perinatal mortality and morbidity are to be further reduced. This can be achieved by direct and indirect methods, i.e. cephalometry and thoracometry using ultrasound on the one hand, and biochemical tests of the function of the fetoplacental unit, on the other. Estriol, pregnandiol and HPL levels and some placental enzymes are here of special interest. In this investigation an attempt was made to asses the diagnostic value of the various parameters and to suggest when they should be used. Jürg KUNZ, born 1942, Zürich, Studied medicine at University of Zürich, 1962-69 Curriculum vitae l Material and methodA total of 83 patients presently, or with a case history of risk hospitalised because of suspect placental insufficiency, was investigated. Twin pregnancies and those with doubtful duration were excluded. In the third trimester of pregnancy the following determinations were made: An average of 4 determinations of the biparietal head diameter using the B-scan method (Vidoson), 10 determinations of estriol and 3 of pregnandiol in urine [13,50] and 6 HPL determinations in the semm [49], 7 determinations of heat stable alkaline phosphatase (HSAP) [9], all per individual case. Biparietal head diameter values were transferred to a Standard curve that was in agreement with the one reported by HOLLÄNDER [42]. Growth retardation was assumed if the last two values showed the same trend with the date being certain and were below two Standard deviations or showed decreased progress. Biochemical parameters were considered to be pathological if they were below 95% of the normal values, with two values being outside this normal ränge or with 3 or more values being continuously below the 95% normal ränge. Maturity was assessed after birth from neurological and somatic signs of maturity according to D u BÖ-WITZ [25]. Infants were diagnosed äs small for date if their birth weight was below the lOth percentile on the LUBCHENKO curve [62]. ResultsFifteen of the 83 newborn were small for dates. Five had a gestational age of less than 37 weeks, 4 in the 35/36 week and one in the 30.5 week. A further 11 prematures was in the group of 68 normotrophic children. However, only 3 out of the 15 small for date infants were below the lower limit of the norm. The remaining 81 (98%) were within nonnal limits, 71 % below the curve for mean values. In twelve SGA infants the head diameter was normal prenatally and increased normally. Fig. 2 shows a very good correlation between weight percentile and the percentage deviation from the normal mean of estriol excretion. In all SGA cases values were found to be decreased. Ten were outside the 95% normal limits and 5 were still at least 40% below those limits. In infants with body weights between the lOth and 50 th percentile estriol values were below, the normal mean in 44%.Weight percentiles and HPL concentration in maternal serum ( Fig. 3) also correlated well. In 8 out of 15 SGA inf...

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“…The procedure of Oakey et al, method B (1967) was carried out, as described by the authors, at the Selly Oak Hospital, Birmingham. The method of Brown et al (1968) was carried out at St. Bartholomew's Hospital, London. This method was adapted to a manual procedure in which cylindrical 25 ml separating funnels were used in place of the semi-automatic extractor, the 3 min.…”

Section: Methodsmentioning

confidence: 99%

“…In the original publication of ua Conaill and Muir the results obtained by the automated procedure were compared with the manual Ittrich method, as modified by Cartlidge et al (1961). Three laboratories in collaboration have now compared the automated procedure with the methods of Oakey et al (1967) andBrown et al (1968). Because the two groups had been stored differently, the results of each group were analysed separately to assess whether storage procedure had influenced the results.…”

mentioning

confidence: 99%

Comparison of an Automated Fluorimetric Method with Two Manual Methods for the Determination of Pregnancy Oestrogens

Muir

Scott

Ryan³

et al. 1972

Ann Clin Biochem

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A Rapid Method for Estimating Oestrogens in Urine Using a Semi-Automatic Extractor

Cited by 417 publications

References 16 publications

Excretory Patterns of Urinary Free 11-Hydroxycortico-Steroids and Total Oestrogens Throughout the Normal Menstrual Cycle

Excretory Patterns of Urinary Free 11-Hydroxycortico-Steroids and Total Oestrogens Throughout the Normal Menstrual Cycle

Ultrasound and biochemical findings in intrauterine growth retardation

Comparison of an Automated Fluorimetric Method with Two Manual Methods for the Determination of Pregnancy Oestrogens

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