A rapid DNA probe test compared to culture methods for identification of subgingival plaque bacteria

Tsai, Ching Yin; Wolff, Larry F.; Germaine, G R; Hodges, Jim

doi:10.1034/j.1600-051x.2003.300109.x

Cited by 12 publications

(8 citation statements)

References 22 publications

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“…Outer membrane antigens from P gingivalis were prepared as described elsewhere (29). Briefly, cells were pelleted from the broth culture medium at 6,500 rpm for 20 minutes in 200‐ml bottles.…”

Section: Methodsmentioning

confidence: 99%

Porphyromonas gingivalis and disease‐related autoantibodies in individuals at increased risk of rheumatoid arthritis

Mikuls¹,

Thiele²,

Deane³

et al. 2012

Arthritis & Rheumatism

200

167

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Objective To examine the relationship of Porphyromonas gingivalis to the presence of autoantibodies in individuals at risk of rheumatoid arthritis (RA). Methods Study participants included the following: 1) a cohort enriched in subjects with HLA–DR4 and 2) subjects at risk of RA by virtue of having a first‐degree relative with RA. None of the study subjects satisfied the American College of Rheumatology 1987 classification criteria for RA. Autoantibodies measured included anti–citrullinated protein antibody (ACPA; by second‐generation anti–cyclic citrullinated peptide antibody enzyme‐linked immunosorbent assay [ELISA]) and rheumatoid factor (RF; by nephelometry or ELISA for IgA, IgM, or IgG isotype). Individuals were considered autoantibody positive (n = 113) if they had ≥1 RA‐related autoantibody; individuals were further categorized as high risk (n = 38) if they had ACPA or positive findings ≥2 assays for RF. Autoantibody‐negative individuals (n = 171) served as a comparator group. Antibody to P gingivalis, P intermedia, and F nucleatum were measured. Associations of bacterial antibodies with group status were examined using logistic regression. Results Anti–P gingivalis concentrations were higher in high‐risk (P = 0.011) and autoantibody positive group (P = 0.010) than in the autoantibody negative group. There were no group differences in anti–P intermedia or anti–F nucleatum concentrations. After multivariable adjustment, anti–P gingivalis concentrations (but not anti–P intermedia or anti–F nucleatum) were significantly associated with autoantibody‐positive and high‐risk status (P < 0.05). Conclusion Immunity to P gingivalis, but not P intermedia or F nucleatum, is significantly associated with the presence of RA‐related autoantibodies in individuals at risk of RA. These results support the hypothesis that infection with P gingivalis may play a central role in the early loss of tolerance to self antigens that occurs in the pathogenesis of RA.

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Section: Methodsmentioning

confidence: 99%

Porphyromonas gingivalis and disease‐related autoantibodies in individuals at increased risk of rheumatoid arthritis

Mikuls¹,

Thiele²,

Deane³

et al. 2012

Arthritis & Rheumatism

200

167

View full text Add to dashboard Cite

show abstract

“…The cells were grown with constant low-speed shaking (150 rpm) at 37°C for 24 hours to an optical density of 1.5 at 660 nm. Sonic extracts of P. gingivalis were prepared as described by Tsai et al (26). An enzyme-linked immunosorbent assay (ELISA) was adapted from the procedure described by Engvall and Perlmann (27).…”

Section: Methodsmentioning

confidence: 99%

Antibody responses to Porphyromonas gingivalis (P. gingivalis) in subjects with rheumatoid arthritis and periodontitis

Mikuls

Payne

Reinhardt

et al. 2009

International Immunopharmacology

243

205

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Summary Antibody titers to P. gingivalis are increased in patients with rheumatoid arthritis and are associated with disease-specific autoimmunity. Background Periodontitis (PD) has been implicated as a risk factor for rheumatoid arthritis (RA). We sought to characterize antibody titers to P. gingivalis (a pathogen in PD) in subjects with RA, PD, and in healthy controls and to examine their relationship with disease autoantibodies. Methods P. gingivalis antibody was measured in subjects with RA (n = 78), PD (n = 39), and in controls (n = 40). Group frequencies of bacterial titer elevations were compared using the Chi-square test and antibody titers were compared using non-parametric tests. Correlations of P. gingivalis titer with C-reactive protein (CRP), antibody to cyclic citrullinated peptide (anti-CCP), and rheumatoid factor (RF) were examined in those with RA while CRP and autoantibody concentrations were compared based on seropositivity to P. gingivalis. Results Antibody titers to P. gingivalis were highest in PD, lowest in controls, and intermediate in RA (p = 0.0003). Elevations in P. gingivalis (titer ≥ 800) were more common in RA and PD (67% and 77%, respectively) than in controls (40%) (p = 0.002). In RA, there were significant correlations with P. gingivalis titer with CRP, anti-CCP-IgM, and -IgG-2. CRP (p = 0.006), anti-CCP-IgM (p = 0.01) and -IgG2 (p = 0.04) concentrations were higher in RA cases with P. gingivalis titers ≥ 800 compared to cases with titers < 800. Conclusion Antibodies to P. gingivalis are more common in RA subjects than controls, although lower than that in PD. Associations of P. gingivalis titers with RA-related autoantibody and CRP concentrations suggests that infection with this organism plays a role in disease risk and progression in RA.

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“…However, this method, used in the identification of Tannerella forsythensis and Porphyromonas gingivalis, demonstrated a detection threshold of 10 5 cells, relatively high when compared to the culture method (Tsai et al 2003).…”

Section: Nucleic Acid Probe Methodsmentioning

confidence: 99%

Diagnosis in Periodontology: A Further Aid Through Microbiological Tests

D’Ercole

Catamo

Piccolomini

2008

Critical Reviews in Microbiology

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Most of the current knowledge of the complex microbiology of oral biofilms, which initiates and maintains periodontal lesions, has been facilitated by the introduction of molecular techniques.Several studies exalt the high sensitivity and specificity of molecular tests in the detection and quantification of periodontal pathogens. Although they have large a diffusion, the old method of bacterial culture remains nowadays the gold standard when determining the utility of a new microbial test. Moreover, cultures have the important advantage of allowing an antibiotic sensitivity test and this is much more important during the treatment of patients with aggressive periodontitis.

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A rapid DNA probe test compared to culture methods for identification of subgingival plaque bacteria

Cited by 12 publications

References 22 publications

Porphyromonas gingivalis and disease‐related autoantibodies in individuals at increased risk of rheumatoid arthritis

Porphyromonas gingivalis and disease‐related autoantibodies in individuals at increased risk of rheumatoid arthritis

Antibody responses to Porphyromonas gingivalis (P. gingivalis) in subjects with rheumatoid arthritis and periodontitis

Diagnosis in Periodontology: A Further Aid Through Microbiological Tests

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